thiC:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
thiC |
|---|---|
| Mnemonic |
Thiamin (and thiazole) |
| Synonyms |
ECK3986, b3994, JW3958[1], JW3958 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
90.36 minutes |
MG1655: 4194122..4192227 |
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NC_012967: 4175710..4173815 |
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NC_012759: 4081907..4083802 |
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W3110 |
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W3110: 3440582..3442477 |
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DH10B: 4293818..4291923 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
thiC(del) (Keio:JW3958) |
deletion |
deletion |
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thiC39::Tn10 |
Insertion at 4,192,588 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[4] |
Synonyms: thi-39::Tn10 | ||||
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thiC39::Tn10 |
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thiC34 |
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thiC3178::Tn10kan |
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thiC43 |
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thiC44 |
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thiC765(del)::kan |
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thiC mutation in PT-R1 |
Resistant to |
Prythiamine resistance developed from mutation in the |
The exact location of the mutation was not located until decades later due to another experiment with the strain[8] | ||||
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thiC mutation in PT-R1 |
Acumulation of metabolite |
Thiamine began to accumulate within pryithiamine resistant strains. |
Strain PT-R3 also had Thiamine accumulation, this would help in the development of pryithiamine resistance. | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3958 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCTGCAACAAAACTGACCCG Primer 2:CCgGCTTCCTCCTTACGCAGGTA | |
|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 19% [4] | ||
|
Linked marker |
est. P1 cotransduction: 11% [4] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 5.0 5.1 Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
- ↑ Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
- ↑ 7.0 7.1 Kawasaki, T & Nose, Y (1969) Thiamine regulatory mutants in Escherichia coli. J. Biochem. 65 417-25 PubMed
- ↑ Sudarsan, N et al. (2005) Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine. Chem. Biol. 12 1325-35 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
