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Product(s) |

Expression|

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Standard Name	trkA
Gene Synonym(s)	ECK3276, b3290, JW3251^[1], JW3251, sapG
Product Desc.	NAD-binding component of TrK potassium transporter^[2]^[3] Major constitutive K+ uptake permeases TrkAG and TrkAH; high-rate, low-affinity transport; peripheral membrane protein bound to inner membrane protein TrkG or TrkH to form active permease; binds NAD+ and NADH^[4]
Product Synonyms(s)	NAD-binding component of TrK potassium transporter^[1], B3290^[2]^[1], TrkA^[2]^[1] , ECK3276, JW3251, sapG, b3290
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): trkA^[2]
Regulation/Activity
Quick Links
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Notes

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Although normally required for Trk activity, overproduction of TrkG or TrkH alone increases K+ transport in a trkA mutant strain (Buurman, 2004). Has two complete TrkA(RCK) domains. SapD(TrkE) is required for TrkAH activity and stimulates TrkAG transport rate (Bossemeyer, 1989; Dosch, 1991; Harms, 2001). Trk-mediated transport is driven by proton motive force, but requires a high ATP concentration for maximal activity, presumably because ATP-binding is needed to activate SapD (Harms, 2001). Anaerobically, TrkAH is proposed to form a membrane supercomplex with the F0F1 ATPase and formate-hydrogen lyase (HycB-HycE-FdhF) responsible for hydrogen gas production and 2H+/K+ exchange; thiol groups are required for this activity (Trchounian, 2000).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	trkA
Mnemonic	Transport K+
Synonyms	ECK3276, b3290, JW3251^[1], JW3251, sapG
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	74.03 minutes	MG1655: 3434540..3435916 <gbrowseImage> name=NC_000913:3434540..3435916 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3364423..3365799 <gbrowseImage> name=NC_012967:3364423..3365799 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3321697..3323073 <gbrowseImage> name=NC_012759:3321697..3323073 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4203898..4202522 <gbrowseImage> name=NC_007779:4202522..4203898 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3434540	Edman degradation	PMID:8412700
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔtrkA (Keio:JW3251)	deletion	deletion	PMID:16738554	Shigen CGSC10452^[5]
trkA::Tn5KAN-I-SceI (FB21104)	Insertion at nt 1082 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21104	contains pKD46
trkA133				CGSC:8151
trkA401				CGSC:11696
trkA405				CGSC:12256
ΔtrkA726::kan			PMID:16738554	CGSC:103210
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3251	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAATTATCATTCTGGGTGC Primer 2:CCtAAGAAGAAAGGGCTTGGCTG
smg-3082::Tn10	Linked marker	CAG12071 = CGSC7444^[5]	est. P1 cotransduction: 82% ^[6] Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 2% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11019	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11019	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001008	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947788	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1012	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010787	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TrkA
Synonyms	NAD-binding component of TrK potassium transporter^[1], B3290^[2]^[1], TrkA^[2]^[1] , ECK3276, JW3251, sapG, b3290
Product description	NAD-binding component of TrK potassium transporter^[2]^[3] Major constitutive K+ uptake permeases TrkAG and TrkAH; high-rate, low-affinity transport; peripheral membrane protein bound to inner membrane protein TrkG or TrkH to form active permease; binds NAD+ and NADH^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005488	binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006811	ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006813	potassium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003148	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006813	potassium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006036	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006813	potassium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006037	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006813	potassium ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0633	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008324	cation transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006036	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008324	cation transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006037	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0030955	potassium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0630	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplC	PMID:16606699	Experiment(s):EBI-1145396
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1145396
Protein	groL	PMID:16606699	Experiment(s):EBI-1145396
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKIIILGAGQ VGGTLAENLV GENNDITVVD TNGERLRTLQ DKFDLRVVQG HGSHPRVLRE AGADDADMLV AVTSSDETNM VACQVAYSLF NTPNRIARIR SPDYVRDADK LFHSDAVPID HLIAPEQLVI DNIYRLIEYP GALQVVNFAE GKVSLAVVKA YYGGPLIGNA LSTMREHMPH IDTRVAAIFR HDRPIRPQGS TIVEAGDEVF FIAASQHIRA VMSELQRLEK PYKRIMLVGG GNIGAGLARR LEKDYSVKLI ERNQQRAAEL AEKLQNTIVF FGDASDQELL AEEHIDQVDL FIAVTNDDEA NIMSAMLAKR MGAKKVMVLI QRRAYVDLVQ GSVIDIAISP QQATISALLS HVRKADIVGV SSLRRGVAEA IEAVAHGDES TSRVVGRVID EIKLPPGTII GAVVRGNDVM IANDNLRIEQ GDHVIMFLTD KKFITDVERL FQPSPFFL
Length	458
Mol. Wt	50.367 kDa
pI	5.8 (calculated)
Extinction coefficient	13,410 - 13,535 (calc based on 9 Y, 0 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	155..226	PF02080 TrkA-C domain	PMID:19920124
Domain	388..452	PF02080 TrkA-C domain	PMID:19920124
Domain	3..125	PF02254 TrkA-N domain	PMID:19920124
Domain	235..350	PF02254 TrkA-N domain	PMID:19920124
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<motif_map/>

Structure
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Structures

<beststructure> gene=trkA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131169	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947788	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010787	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AGI8	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11019	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11019	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947788	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001008	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1012	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	563	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	153	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	164	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
trkA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3434520..3434560 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3290 (EcoliWiki Page)	NCBI GEO profiles for trkA
microarray	GenExpDB:b3290 (EcoliWiki Page)	Summary of data for trkA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3434038..3434193) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ11; Well:F5^[7]
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Notes

Accessions Related to trkA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11019	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1012	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3290
EcoGene	EcoGene:EG11019	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001008	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010787	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	TRKA	From SHIGELLACYC
E. coli O157	TRKA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:51735
Superfamily (EcoliWiki Page)	SUPERFAMILY:51735
Superfamily (EcoliWiki Page)	SUPERFAMILY:116726
Superfamily (EcoliWiki Page)	SUPERFAMILY:116726
Pfam (EcoliWiki Page)	PF02080 TrkA-C domain
Pfam (EcoliWiki Page)	PF02080 TrkA-C domain
Pfam (EcoliWiki Page)	PF02254 TrkA-N domain
Pfam (EcoliWiki Page)	PF02254 TrkA-N domain
EcoCyc	EcoCyc:EG11019	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11019	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001008	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1012	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010787	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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Gene

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Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to trkA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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