{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	topB
Gene Synonym(s)	ECK1761, b1763, JW1752, mutR^[1], mutR
Product Desc.	DNA topoisomerase III^[2]^[3] Topoisomerase III; mutR phenotype is decreased deletion formation between short repeats^[4]
Product Synonyms(s)	DNA topoisomerase III^[1], MutR^[2]^[1], B1763^[2]^[1], TopB^[2]^[1], Topo III^[2]^[1] , ECK1761, JW1752, mutR, b1763
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ydjA-selD-topB^[2]
Regulation/Activity
Quick Links
edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	topB
Mnemonic	Topoisomerase
Synonyms	ECK1761, b1763, JW1752, mutR^[1], mutR
edit table

Notes

Location(s) and DNA Sequence

[back to top]

See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	39.72 minutes	MG1655: 1844984..1843023 <gbrowseImage> name=NC_000913:1843023..1844984 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1824188..1822227 <gbrowseImage> name=NC_012967:1822227..1824188 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1735082..1737043 <gbrowseImage> name=NC_012759:1735082..1737043 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1848674..1846713 <gbrowseImage> name=NC_007779:1846713..1848674 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1935555..1933594 <gbrowseImage> name=NC_010473:1933594..1935555 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1843023	Edman degradation	PMID:2553698
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔtopB (Keio:JW1752)		deletion			PMID:16738554	Shigen CGSC9474^[5]
topB::Tn5KAN-2 (FB20451)	Insertion at nt 943 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20451	contains pKD46

topB77::Tn5						CGSC:80894
ΔtopB761::kan					PMID:16738554	CGSC:103174
ΔtopB::aphA					PMID:11493711
ΔtopA ΔtopB			Growth Phenotype	Highly filamented over time Aberrant nucleoid structures	PMID:11493711		depletion experiment- contained complementing topB expressing plasmid
ΔtopA ΔtopB ΔrecA				no chromosomal segregation defect partially suppressed filamentation	PMID:11493711		complements the ΔtopA ΔtopB phenotype
ΔtopB::kan parE(ts)			Growth Phenotype	synthetically lethal	PMID:16164551
ΔtopB::kan parC(ts)			Growth Phenotype	synthetically lethal	PMID:16164551
ΔtopBruvC53		deletion	Sensitivity to	~20 times more sensitive to UV irradiation than the single ruvC53 mutation alone. MMC	PMID:16164551
ΔtopBΔtopA				chromosomal segregation defect is due to a defect in recombination, not decatenation	PMID:11493711
edit table

Notes

topB is cool

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1752	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGGTTGTTTATTGCCGAAAA Primer 2:CCgGCTATCGCCCCGCTTCCGAC
4B8	Kohara Phage	Genobase	PMID:3038334
12H7	Kohara Phage	Genobase	PMID:3038334
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 70% ^[6] Synonyms:zdi-276::Tn10
zea-225::Tn10	Linked marker	CAG18465 = CGSC7388^[5]	est. P1 cotransduction: 36% ^[6] Synonyms:zdj-225::Tn10
pTBE302	Plasmid Clone		pBAD- regulated XPN of topB PMID:11493711
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11014	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11014	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001003	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946141	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1007	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005869	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TopB
Synonyms	DNA topoisomerase III^[1], MutR^[2]^[1], B1763^[2]^[1], TopB^[2]^[1], Topo III^[2]^[1] , ECK1761, JW1752, mutR, b1763
Product description	DNA topoisomerase III^[2]^[3] Topoisomerase III; mutR phenotype is decreased deletion formation between short repeats^[4]
EC number (for enzymes)	5.99.1.2^[1]
edit table

Notes

Function

[back to top]

Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003917	DNA topoisomerase type I activity	PMID:6326814^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0003676	nucleic acid binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006154	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000380	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003601	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003602	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005738	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013497	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013825	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003916	DNA topoisomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003601	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003916	DNA topoisomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003602	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003916	DNA topoisomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005738	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003916	DNA topoisomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013497	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003916	DNA topoisomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013825	F	Seeded from EcoCyc (v14.0)	complete
	GO:0007059	chromosome segregation	PMID:11493711^[8]	IGI: Inferred from Genetic Interaction		P		Missing: with/from
	GO:0003916	DNA topoisomerase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0799	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003917	DNA topoisomerase type I activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000380	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003917	DNA topoisomerase type I activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.99.1.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005694	chromosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000380	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005694	chromosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003601	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005694	chromosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003602	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005694	chromosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005738	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005694	chromosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013497	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005694	chromosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013825	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006259	DNA metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006154	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006265	DNA topological change	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000380	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006265	DNA topological change	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003601	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006265	DNA topological change	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003602	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006265	DNA topological change	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005738	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006265	DNA topological change	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013497	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006265	DNA topological change	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013825	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006268	DNA unwinding involved in replication	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013825	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016853	isomerase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0413	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	PMID:16164551^[9]	IMP: Inferred from Mutant Phenotype		P	involved in processing intermediates in an alternate pathway to RuvC	complete
	GO:0051304	chromosome separation	PMID:12509418^[10]	IMP: Inferred from Mutant Phenotype		P	very efficient in removing precatenanes	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	aceE	PMID:15690043^[11]	Experiment(s):EBI-888082
Protein	aceF	PMID:15690043^[11]	Experiment(s):EBI-888082, EBI-893650
Protein	lpdA	PMID:15690043^[11]	Experiment(s):EBI-888082, EBI-893650
Protein	recD	PMID:15690043^[11]	Experiment(s):EBI-888082
Protein	recQ	PMID:15690043^[11] PMID:10360177^[12]	Experiment(s):EBI-888082
Protein	rplC	PMID:15690043^[11]	Experiment(s):EBI-888082, EBI-893650
Protein	rpsB	PMID:15690043^[11]	Experiment(s):EBI-888082, EBI-893650
Protein	rpsC	PMID:15690043^[11]	Experiment(s):EBI-888082
Protein	topA	PMID:15690043^[11]	Experiment(s):EBI-888082, EBI-888130
Protein	ccmB	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplU	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplV	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplX	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpmB	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpmC	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpmG	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpsE	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpsN	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpsP	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	hscC	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	abgT	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	yjcD	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	gcl	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	hupA	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplD	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplJ	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplL	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplM	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rplS	PMID:15690043^[11]	Experiment(s):EBI-893650
Protein	rpsP	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	rplU	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	rpsC	PMID:19402753^[13]	LCMS(ID Probability):97.9 MALDI(Z-score):9.123007
Protein	rplS	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	rplM	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	rpsN	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	hupA	PMID:19402753^[13]	LCMS(ID Probability):99.3
Protein	rplD	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	topA	PMID:19402753^[13]	MALDI(Z-score):25.567635
Protein	ssb	PMID:19402753^[13]	LCMS(ID Probability):99.6 MALDI(Z-score):39.717962
Protein	recQ	PMID:19402753^[13]	MALDI(Z-score):27.205314
Protein	gcl	PMID:19402753^[13]	LCMS(ID Probability):99.0
Protein	abgT	PMID:19402753^[13]	LCMS(ID Probability):99.6
Protein	yjcD	PMID:19402753^[13]	LCMS(ID Probability):99.6
edit table

Notes

A model for Topo III's role in the processing of recombination intermediates may be found in Lopez et al. (2005)^[9].

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRLFIAEKPS LARAIADVLP KPHRKGDGFI ECGNGQVVTW CIGHLLEQAQ PDAYDSRYAR WNLADLPIVP EKWQLQPRPS VTKQLNVIKR FLHEASEIVH AGDPDREGQL LVDEVLDYLQ LAPEKRQQVQ RCLINDLNPQ AVERAIDRLR SNSEFVPLCV SALARARADW LYGINMTRAY TILGRNAGYQ GVLSVGRVQT PVLGLVVRRD EEIENFVAKD FFEVKAHIVT PADERFTAIW QPSEACEPYQ DEEGRLLHRP LAEHVVNRIS GQPAIVTSYN DKRESESAPL PFSLSALQIE AAKRFGLSAQ NVLDICQKLY ETHKLITYPR SDCRYLPEEH FAGRHAVMNA ISVHAPDLLP QPVVDPDIRN RCWDDKKVDA HHAIIPTARS SAINLTENEA KVYNLIARQY LMQFCPDAVF RKCVIELDIA KGKFVAKARF LAEAGWRTLL GSKERDEEND GTPLPVVAKG DELLCEKGEV VERQTQPPRH FTDATLLSAM TGIARFVQDK DLKKILRATD GLGTEATRAG IIELLFKRGF LTKKGRYIHS TDAGKALFHS LPEMATRPDM TAHWESVLTQ ISEKQCRYQD FMQPLVGTLY QLIDQAKRTP VRQFRGIVAP GSGGSADKKK AAPRKRSAKK SPPADEVGSG AIA
Length	653
Mol. Wt	73.217 kDa
pI	8.3 (calculated)
Extinction coefficient	67,840 - 69,340 (calc based on 16 Y, 8 W, and 12 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	153..593	PF01131 DNA topoisomerase	PMID:19920124^[14]
Domain	1..135	PF01751 Toprim domain	PMID:19920124^[14]
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=topB taxon=562,83333 </beststructure>

Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129717	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946141	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005869	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P14294	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11014	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11014	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946141	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001003	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1007	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	8.997+/-0.083	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.051759306	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	419	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	100	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	200	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ydjA-selD-topB
edit table

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<gbrowseImage> name=NC_000913:1844964..1845004 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1763 (EcoliWiki Page)	NCBI GEO profiles for topB
microarray	GenExpDB:b1763 (EcoliWiki Page)	Summary of data for topB from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to topB Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11014	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1007	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1763
EcoGene	EcoGene:EG11014	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001003	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005869	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001725 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G63920 (score: 1.000; bootstrap: 100%) AT2G32000 (score: 0.143)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00004532001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000027497 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	TOPB	From SHIGELLACYC
E. coli O157	TOPB	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:56712
Pfam (EcoliWiki Page)	PF01131 DNA topoisomerase
Pfam (EcoliWiki Page)	PF01751 Toprim domain
EcoCyc	EcoCyc:EG11014	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11014	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001003	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1007	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005869	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Srivenugopal, KS et al. (1984) Escherichia coli DNA topoisomerase III: purification and characterization of a new type I enzyme. Biochemistry 23 1899-906 PubMed
↑ Zhu, Q et al. (2001) Type I topoisomerase activity is required for proper chromosomal segregation in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 98 9766-71 PubMed
↑ ^9.0 ^9.1 Lopez, CR et al. (2005) A role for topoisomerase III in a recombination pathway alternative to RuvABC. Mol. Microbiol. 58 80-101 PubMed
↑ Nurse, P et al. (2003) Topoisomerase III can serve as the cellular decatenase in Escherichia coli. J. Biol. Chem. 278 8653-60 PubMed
↑ ^11.00 ^11.01 ^11.02 ^11.03 ^11.04 ^11.05 ^11.06 ^11.07 ^11.08 ^11.09 ^11.10 ^11.11 ^11.12 ^11.13 ^11.14 ^11.15 ^11.16 ^11.17 ^11.18 ^11.19 ^11.20 ^11.21 ^11.22 ^11.23 ^11.24 ^11.25 ^11.26 ^11.27 ^11.28 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
↑ Harmon, FG et al. (1999) RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination. Mol. Cell 3 611-20 PubMed
↑ ^13.00 ^13.01 ^13.02 ^13.03 ^13.04 ^13.05 ^13.06 ^13.07 ^13.08 ^13.09 ^13.10 ^13.11 ^13.12 ^13.13 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
↑ ^14.0 ^14.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

topB:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to topB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search