topB:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
TopB |
|---|---|
| Synonyms |
DNA topoisomerase III[1], MutR[2][1], B1763[2][1], TopB[2][1], Topo III[2][1] , ECK1761, JW1752, mutR, b1763 |
| Product description |
Topoisomerase III; mutR phenotype is decreased deletion formation between short repeats[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003917 |
DNA topoisomerase type I activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
IGI: Inferred from Genetic Interaction |
P |
Missing: with/from | ||||
| GO:0003916 |
DNA topoisomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003917 |
DNA topoisomerase type I activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003917 |
DNA topoisomerase type I activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006259 |
DNA metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006268 |
DNA unwinding involved in replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016853 |
isomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006310 |
DNA recombination |
IMP: Inferred from Mutant Phenotype |
P |
involved in processing intermediates in an alternate pathway to RuvC |
complete | |||
| GO:0051304 |
chromosome separation |
IMP: Inferred from Mutant Phenotype |
P |
very efficient in removing precatenanes |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
aceE |
Experiment(s):EBI-888082 | ||
|
Protein |
aceF |
Experiment(s):EBI-888082, EBI-893650 | ||
|
Protein |
lpdA |
Experiment(s):EBI-888082, EBI-893650 | ||
|
Protein |
recD |
Experiment(s):EBI-888082 | ||
|
Protein |
recQ |
Experiment(s):EBI-888082 | ||
|
Protein |
rplC |
Experiment(s):EBI-888082, EBI-893650 | ||
|
Protein |
rpsB |
Experiment(s):EBI-888082, EBI-893650 | ||
|
Protein |
rpsC |
Experiment(s):EBI-888082 | ||
|
Protein |
topA |
Experiment(s):EBI-888082, EBI-888130 | ||
|
Protein |
ccmB |
Experiment(s):EBI-893650 | ||
|
Protein |
rplU |
Experiment(s):EBI-893650 | ||
|
Protein |
rplV |
Experiment(s):EBI-893650 | ||
|
Protein |
rplX |
Experiment(s):EBI-893650 | ||
|
Protein |
rpmB |
Experiment(s):EBI-893650 | ||
|
Protein |
rpmC |
Experiment(s):EBI-893650 | ||
|
Protein |
rpmG |
Experiment(s):EBI-893650 | ||
|
Protein |
rpsE |
Experiment(s):EBI-893650 | ||
|
Protein |
rpsN |
Experiment(s):EBI-893650 | ||
|
Protein |
rpsP |
Experiment(s):EBI-893650 | ||
|
Protein |
hscC |
Experiment(s):EBI-893650 | ||
|
Protein |
abgT |
Experiment(s):EBI-893650 | ||
|
Protein |
yjcD |
Experiment(s):EBI-893650 | ||
|
Protein |
gcl |
Experiment(s):EBI-893650 | ||
|
Protein |
hupA |
Experiment(s):EBI-893650 | ||
|
Protein |
rplD |
Experiment(s):EBI-893650 | ||
|
Protein |
rplJ |
Experiment(s):EBI-893650 | ||
|
Protein |
rplL |
Experiment(s):EBI-893650 | ||
|
Protein |
rplM |
Experiment(s):EBI-893650 | ||
|
Protein |
rplS |
Experiment(s):EBI-893650 | ||
|
Protein |
rpsP |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplU |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsC |
LCMS(ID Probability):97.9 MALDI(Z-score):9.123007 | ||
|
Protein |
rplS |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplM |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsN |
LCMS(ID Probability):99.6 | ||
|
Protein |
hupA |
LCMS(ID Probability):99.3 | ||
|
Protein |
rplD |
LCMS(ID Probability):99.6 | ||
|
Protein |
topA |
MALDI(Z-score):25.567635 | ||
|
Protein |
ssb |
LCMS(ID Probability):99.6 MALDI(Z-score):39.717962 | ||
|
Protein |
recQ |
MALDI(Z-score):27.205314 | ||
|
Protein |
gcl |
LCMS(ID Probability):99.0 | ||
|
Protein |
abgT |
LCMS(ID Probability):99.6 | ||
|
Protein |
yjcD |
LCMS(ID Probability):99.6 | ||
| edit table |
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Notes
- A model for Topo III's role in the processing of recombination intermediates may be found in Lopez et al. (2005)[7].
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MRLFIAEKPS LARAIADVLP KPHRKGDGFI ECGNGQVVTW CIGHLLEQAQ PDAYDSRYAR WNLADLPIVP EKWQLQPRPS VTKQLNVIKR FLHEASEIVH AGDPDREGQL LVDEVLDYLQ LAPEKRQQVQ RCLINDLNPQ AVERAIDRLR SNSEFVPLCV SALARARADW LYGINMTRAY TILGRNAGYQ GVLSVGRVQT PVLGLVVRRD EEIENFVAKD FFEVKAHIVT PADERFTAIW QPSEACEPYQ DEEGRLLHRP LAEHVVNRIS GQPAIVTSYN DKRESESAPL PFSLSALQIE AAKRFGLSAQ NVLDICQKLY ETHKLITYPR SDCRYLPEEH FAGRHAVMNA ISVHAPDLLP QPVVDPDIRN RCWDDKKVDA HHAIIPTARS SAINLTENEA KVYNLIARQY LMQFCPDAVF RKCVIELDIA KGKFVAKARF LAEAGWRTLL GSKERDEEND GTPLPVVAKG DELLCEKGEV VERQTQPPRH FTDATLLSAM TGIARFVQDK DLKKILRATD GLGTEATRAG IIELLFKRGF LTKKGRYIHS TDAGKALFHS LPEMATRPDM TAHWESVLTQ ISEKQCRYQD FMQPLVGTLY QLIDQAKRTP VRQFRGIVAP GSGGSADKKK AAPRKRSAKK SPPADEVGSG AIA |
| Length |
653 |
| Mol. Wt |
73.217 kDa |
| pI |
8.3 (calculated) |
| Extinction coefficient |
67,840 - 69,340 (calc based on 16 Y, 8 W, and 12 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Srivenugopal, KS et al. (1984) Escherichia coli DNA topoisomerase III: purification and characterization of a new type I enzyme. Biochemistry 23 1899-906 PubMed
- ↑ Zhu, Q et al. (2001) Type I topoisomerase activity is required for proper chromosomal segregation in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 98 9766-71 PubMed
- ↑ 7.0 7.1 Lopez, CR et al. (2005) A role for topoisomerase III in a recombination pathway alternative to RuvABC. Mol. Microbiol. 58 80-101 PubMed
- ↑ Nurse, P et al. (2003) Topoisomerase III can serve as the cellular decatenase in Escherichia coli. J. Biol. Chem. 278 8653-60 PubMed
- ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 9.18 9.19 9.20 9.21 9.22 9.23 9.24 9.25 9.26 9.27 9.28 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ Harmon, FG et al. (1999) RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination. Mol. Cell 3 611-20 PubMed
- ↑ 11.00 11.01 11.02 11.03 11.04 11.05 11.06 11.07 11.08 11.09 11.10 11.11 11.12 11.13 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 12.0 12.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000166 ! nucleotide binding
- GO:0003917 ! DNA topoisomerase type I activity
- GO:0003676 ! nucleic acid binding
- GO:0003677 ! DNA binding
- GO:0003916 ! DNA topoisomerase activity
- GO:0007059 ! chromosome segregation
- GO:0005524 ! ATP binding
- GO:0005694 ! chromosome
- GO:0006259 ! DNA metabolic process
- GO:0006265 ! DNA topological change
- GO:0006268 ! DNA unwinding involved in DNA replication
- GO:0016853 ! isomerase activity
- GO:0006310 ! DNA recombination
- GO:0051304 ! chromosome separation
- Proteins
