ruvB:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
ruvB |
---|---|
Gene Synonym(s) |
ECK1861, b1860, JW1849[1], JW1849 |
Product Desc. |
branch migration of Holliday structures; repair helicase[2][3]; Component of resolvasome[2][3] DNA helicase driving branch migration during recombination; hexameric ring[4] |
Product Synonyms(s) |
ATP-dependent DNA helicase, component of RuvABC resolvasome[1], B1860[2][1], RuvB[2][1] , ECK1861, JW1849, b1860 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
May directly convert replication forks into Holliday junctions. Required for replication forks reversal in certain replication mutants. ATP-dependent. LexA regulon.[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
ruvB |
---|---|
Mnemonic |
Resistance to UV light |
Synonyms |
ECK1861, b1860, JW1849[1], JW1849 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
41.86 minutes, 41.86 minutes |
MG1655: 1943380..1942370 |
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NC_012967: 1924126..1923116 |
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NC_012759: 1834429..1835439 |
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W3110 |
|
W3110: 1947070..1946060 |
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DH10B: 2033951..2032941 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔruvB (Keio:JW1849) |
deletion |
deletion |
PMID:16738554 |
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ΔruvB::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
PMID:16095938 |
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ruvB52 |
|||||||
ΔruvB785::kan |
PMID:16738554 |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW1849 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCATTGAAGCAGACCGTCTGAT Primer 2:CCtGGCATTTCTGGCGGCGTTAT | |
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 65% [6] | ||
Linked marker |
est. P1 cotransduction: 11% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:RUVB |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10924 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120004476 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0917 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0006204 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
RuvB |
---|---|
Synonyms |
ATP-dependent DNA helicase, component of RuvABC resolvasome[1], B1860[2][1], RuvB[2][1] , ECK1861, JW1849, b1860 |
Product description |
branch migration of Holliday structures; repair helicase[2][3]; Component of resolvasome[2][3] DNA helicase driving branch migration during recombination; hexameric ring[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003593 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004605 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008823 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004386 |
helicase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0347 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00016 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004605 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00016 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004605 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008823 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008824 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0234 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00016 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004605 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008823 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008824 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0233 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006974 |
response to DNA damage stimulus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0227 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009378 |
four-way junction helicase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00016 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009378 |
four-way junction helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004605 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009378 |
four-way junction helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008823 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009378 |
four-way junction helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008824 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009432 |
SOS response |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00016 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009432 |
SOS response |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0742 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016787 |
hydrolase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0378 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0017111 |
nucleoside-triphosphatase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003593 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of resolvasome |
could be indirect |
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Protein |
dnaJ |
PMID:15690043 |
Experiment(s):EBI-880906, EBI-887115 | |
Protein |
dnaK |
PMID:15690043 |
Experiment(s):EBI-880906, EBI-887115 | |
Protein |
groL |
PMID:15690043 |
Experiment(s):EBI-880906, EBI-887115 | |
Protein |
clpB |
PMID:15690043 |
Experiment(s):EBI-887115 | |
Protein |
rho |
PMID:15690043 |
Experiment(s):EBI-887115 | |
Protein |
rpsB |
PMID:15690043 |
Experiment(s):EBI-887115 | |
Protein |
rpsJ |
PMID:15690043 |
Experiment(s):EBI-887115 | |
Protein |
tufA |
PMID:15690043 |
Experiment(s):EBI-887115 | |
Protein |
groL |
PMID:16606699 |
Experiment(s):EBI-1141073 | |
Protein |
adhE |
PMID:16606699 |
Experiment(s):EBI-1141073 | |
Protein |
aceE |
PMID:16606699 |
Experiment(s):EBI-1141073 | |
Protein |
rpsA |
PMID:16606699 |
Experiment(s):EBI-1141073 | |
Protein |
yojN |
PMID:16606699 |
Experiment(s):EBI-1141073 | |
Protein |
dnaK |
PMID:16606699 |
Experiment(s):EBI-1141073 | |
Protein |
groL |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | |
Protein |
rho |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
groS |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):7.120704 | |
Protein |
clpB |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
dnaJ |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):26.138101 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MIEADRLISA GTTLPEDVAD RAIRPKLLEE YVGQPQVRSQ MEIFIKAAKL RGDALDHLLI FGPPGLGKTT LANIVANEMG VNLRTTSGPV LEKAGDLAAM LTNLEPHDVL FIDEIHRLSP VVEEVLYPAM EDYQLDIMIG EGPAARSIKI DLPPFTLIGA TTRAGSLTSP LRDRFGIVQR LEFYQVPDLQ YIVSRSARFM GLEMSDDGAL EVARRARGTP RIANRLLRRV RDFAEVKHDG TISADIAAQA LDMLNVDAEG FDYMDRKLLL AVIDKFFGGP VGLDNLAAAI GEERETIEDV LEPYLIQQGF LQRTPRGRMA TTRAWNHFGI TPPEMP |
Length |
336 |
Mol. Wt |
37.175 kDa |
pI |
4.9 (calculated) |
Extinction coefficient |
15,930 (calc based on 7 Y, 1 W, and 0 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0006204 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:RUVB |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10924 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120004476 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0917 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
1.60E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
E. coli K-12 MG1655 |
452 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
196 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
218 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:1943360..1943400
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for ruvB | |
microarray |
Summary of data for ruvB from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to ruvB Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:RUVB |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0917 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10924 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120004476 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0006204 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Drosophila melanogaster |
|
From Inparanoid:20070104 |
Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Rattus norvegicus |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
Shigella flexneri |
RUVB |
From SHIGELLACYC |
E. coli O157 |
RUVB |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:RUVB |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10924 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120004476 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0917 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0006204 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
Categories
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Drosophila pseudoobscura
- Genes with homologs in Homo sapiens
- Genes with homologs in Oryza gramene
- Genes with homologs in Rattus norvegicus
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157