ruvA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

ruvA

Gene Synonym(s)

ECK1862, b1861, JW1850[1], JW1850

Product Desc.

branch migration of Holliday structures; repair[2][3];

Component of resolvasome[2][3]

Holliday junction recognition factor, recruits RuvB; works in conjunction with RuvB helicase to drive branch migration during recombination[4]

Product Synonyms(s)

component of RuvABC resolvasome, regulatory subunit[1], B1861[2][1], RuvA[2][1] , ECK1862, JW1850, b1861

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ruvAB[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Binds Holliday junction as two tetramers (an octamer) and each tetrameric half recruits one RuvB hexameric helicase. RuvAB may also directly convert replication forks into Holliday junctions. Required for replication forks reversal in certain replication mutants. LexA regulon. RuvA may be a sliding collar that protects Holliday junctions from unwinding while promoting branch migration[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ruvA

Mnemonic

Resistance to UV light

Synonyms

ECK1862, b1861, JW1850[1], JW1850

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

41.89 minutes 

MG1655: 1944000..1943389
<gbrowseImage> name=NC_000913:1943389..1944000 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1924746..1924135
<gbrowseImage> name=NC_012967:1924135..1924746 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1835448..1836059
<gbrowseImage> name=NC_012759:1835448..1836059 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1947690..1947079
<gbrowseImage> name=NC_007779:1947079..1947690 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2034571..2033960
<gbrowseImage> name=NC_010473:2033960..2034571 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔruvA (Keio:JW1850)

deletion

deletion

PMID:16738554

Shigen
CGSC9544[5]

ruvA::Tn5KAN-2 (FB20477)

Insertion at nt 277 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20477

does not contain pKD46

ruvA::Tn5KAN-2 (FB20478)

Insertion at nt 277 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20478

contains pKD46

ruvA4

CGSC:11226

ruvA200

CGSC:24926

ruvA60(polar)::Tn10

CGSC:24927

ΔruvA786::kan

PMID:16738554

CGSC:102852


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1850

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATAGGCAGACTCAGAGGCAT

Primer 2:CCTAACGCGGCGCGTAGGGCTTC

20H4

Kohara Phage

Genobase

PMID:3038334

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[5]

est. P1 cotransduction: 63% [6]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[5]

est. P1 cotransduction: 12% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:RUVA

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10923

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004475

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946369

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0916

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006206

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RuvA

Synonyms

component of RuvABC resolvasome, regulatory subunit[1], B1861[2][1], RuvA[2][1] , ECK1862, JW1850, b1861

Product description

branch migration of Holliday structures; repair[2][3];

Component of resolvasome[2][3]

Holliday junction recognition factor, recruits RuvB; works in conjunction with RuvB helicase to drive branch migration during recombination[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003583

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003678

DNA helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000085

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0347

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011114

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013849

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00031

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000085

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003583

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011114

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013849

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00031

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000085

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011114

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013849

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0009378

four-way junction helicase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00031

F

Seeded from EcoCyc (v14.0)

complete

GO:0009378

four-way junction helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011114

F

Seeded from EcoCyc (v14.0)

complete

GO:0009378

four-way junction helicase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013849

F

Seeded from EcoCyc (v14.0)

complete

GO:0009379

Holliday junction helicase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011114

C

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00031

P

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0742

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of resolvasome

could be indirect

Protein

rplW

PMID:16606699

Experiment(s):EBI-1141081

Protein

nadE

PMID:16606699

Experiment(s):EBI-1141081

Protein

crl

PMID:16606699

Experiment(s):EBI-1141081

Protein

tadA

PMID:16606699

Experiment(s):EBI-1141081

Protein

tatE

PMID:16606699

Experiment(s):EBI-1141081

Protein

rluB

PMID:19402753

LCMS(ID Probability):99.6

Protein

ybeZ

PMID:19402753

MALDI(Z-score):36.138884

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIGRLRGIII EKQPPLVLIE VGGVGYEVHM PMTCFYELPE AGQEAIVFTH FVVREDAQLL
YGFNNKQERT LFKELIKTNG VGPKLALAIL SGMSAQQFVN AVEREEVGAL VKLPGIGKKT
AERLIVEMKD RFKGLHGDLF TPAADLVLTS PASPATDDAE QEAVAALVAL GYKPQEASRM
VSKIARPDAS SETLIREALR AAL
Length

203

Mol. Wt

22.085 kDa

pI

5.9 (calculated)

Extinction coefficient

5,960 - 6,085 (calc based on 4 Y, 0 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..62

PF01330 RuvA N terminal domain

PMID:19920124

Domain

158..203

PF07499 RuvA, C-terminal domain

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ruvA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129814

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946369

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006206

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A809

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:RUVA

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10923

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946369

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004475

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0916

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

657

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

249

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

384

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ruvAB

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1943980..1944020 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1861 (EcoliWiki Page)

NCBI GEO profiles for ruvA

microarray

GenExpDB:b1861 (EcoliWiki Page)

Summary of data for ruvA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1943919..1944201) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:D7[7]

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Notes

Accessions Related to ruvA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:RUVA

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0916

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1861

EcoGene

EcoGene:EG10923

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004475

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006206

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

RUVA

From SHIGELLACYC

E. coli O157

RUVA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:46929

Superfamily (EcoliWiki Page)

SUPERFAMILY:47781

Superfamily (EcoliWiki Page)

SUPERFAMILY:50249

Pfam (EcoliWiki Page)

PF01330 RuvA N terminal domain

Pfam (EcoliWiki Page)

PF07499 RuvA, C-terminal domain

EcoCyc

EcoCyc:RUVA

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10923

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004475

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0916

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006206

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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