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Standard Name	rseA
Gene Synonym(s)	ECK2570, b2572, JW2556, mclA, yfiJ^[1], yfiJ
Product Desc.	anti-sigma factor that inhibits sigmaE^[2]; anti-sigma factor^[3] Anti-RpoE sigma factor, spans inner membrane^[4]
Product Synonyms(s)	anti-sigma factor^[1], B2572^[2]^[1], YfiJ^[2]^[1], MclA^[2]^[1], RseA^[2]^[1] , ECK2570, JW2556, mclA, yfiJ, b2572
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rpoE-rseABC^[2], rseABC^[2]
Regulation/Activity
Quick Links
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Notes

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The anti-sigma N-terminal RseA domain is linked to the periplasmic domain by a single membrane domain spanning residues 100-118. The periplasmic domain is first degraded by DegS. Subsequent cytoplasmic domain degradation requires YaeL. RseA Tyr-46 is phoshorylated. de-phosphorylated by Etp. rseA is transiently induced after a cold shock, dependent on the presence of PNPase. Mutants have elevated OM vesiculation (McBroom, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	rseA
Mnemonic	Regulation of sigma E
Synonyms	ECK2570, b2572, JW2556, mclA, yfiJ^[1], yfiJ
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	58.34 minutes	MG1655: 2707426..2706776 <gbrowseImage> name=NC_000913:2706776..2707426 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2630897..2630247 <gbrowseImage> name=NC_012967:2630247..2630897 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2592581..2593231 <gbrowseImage> name=NC_012759:2592581..2593231 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2708060..2707410 <gbrowseImage> name=NC_007779:2707410..2708060 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2799191..2798541 <gbrowseImage> name=NC_010473:2798541..2799191 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
rseA(del) (Keio:JW2556)	deletion	deletion			PMID:16738554	Shigen CGSC10032^[5]
rseA::Tn5KAN-I-SceI (FB20857)	Insertion at nt 413 in Minus orientation				PMID:15262929	E. coli Genome Project:FB20857	does not contain pKD46
rseA::Tn5KAN-I-SceI (FB20858)	Insertion at nt 413 in Minus orientation				PMID:15262929	E. coli Genome Project:FB20858	contains pKD46
ΔrseA::kan	deletion		Biolog:respiration	unable to respire D-Galacturonate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
rseA(del)::kan	deletion		Biolog:respiration	unable to respire L-Alanyl-glycine	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire Glycyl-L-aspartate	PMID:16095938
ΔrseA::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
ΔrseA741::kan					PMID:16738554	CGSC:102808
resA(del)	deletion		Change in the expression of a gene	The expression of rybB is increased in both the Exponential and Stationary phase	PMID:17416652		See Figure 3 A.
CAG45114 rseA(del)::FRTKanFRT	deletion		Sigma^E activity	Increase in Sigma^E activity	PMID:23224554	Parental Strain: CAG45114 Experimental Strain: SMR15754	See table S11 for full experimental data.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2556	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAGAAAGAACAACTTTCCGC Primer 2:CCtTGCGATTGCGTTCCTAAAGT
4A12	Kohara Phage	Genobase	PMID:3038334
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 15% ^[6] Synonyms:zff-208::Tn10
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 91% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12341	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12341	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002216	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947053	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2245	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008462	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RseA
Synonyms	anti-sigma factor^[1], B2572^[2]^[1], YfiJ^[2]^[1], MclA^[2]^[1], RseA^[2]^[1] , ECK2570, JW2556, mclA, yfiJ, b2572
Product description	anti-sigma factor that inhibits sigmaE^[2]; anti-sigma factor^[3] Anti-RpoE sigma factor, spans inner membrane^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005572	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9904	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016989	sigma factor antagonist activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005572	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQKEQLSALM DGETLDSELL NELAHNPEMQ KTWESYHLIR DSMRGDTPEV LHFDISSRVM AAIEEEPVRQ PATLIPEAQP APHQWQKMPF WQKVRPWAAQ LTQMGVAACV SLAVIVGVQH YNGQSETSQQ PETPVFNTLP MMGKASPVSL GVPSEATANN GQQQQVQEQR RRINAMLQDY ELQRRLHSEQ LQFEQAQTQQ AAVQVPGIQT LGTQSQ
Length	216
Mol. Wt	24.319 kDa
pI	5.0 (calculated)
Extinction coefficient	26,470 - 26,595 (calc based on 3 Y, 4 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	131..186	PF03873 Anti sigma-E protein RseA, C-terminal domain	PMID:19920124
Domain	1..89	PF03872 Anti sigma-E protein RseA, N-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rseA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130497	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947053	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008462	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFX7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12341	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12341	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947053	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002216	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2245	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1136	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1576	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1582	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rpoE-rseABC
rseABC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2707406..2707446 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2572 (EcoliWiki Page)	NCBI GEO profiles for rseA
microarray	GenExpDB:b2572 (EcoliWiki Page)	Summary of data for rseA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to rseA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12341	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2245	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2572
EcoGene	EcoGene:EG12341	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002216	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008462	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	RSEA	From SHIGELLACYC
E. coli O157	RSEA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:89069
Pfam (EcoliWiki Page)	PF03873 Anti sigma-E protein RseA, C-terminal domain
Pfam (EcoliWiki Page)	PF03872 Anti sigma-E protein RseA, N-terminal domain
EcoCyc	EcoCyc:EG12341	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12341	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002216	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2245	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008462	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

rseA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to rseA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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