rseA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rseA

Mnemonic

Regulation of sigma E

Synonyms

ECK2570, b2572, JW2556, mclA, yfiJ[1], yfiJ

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

58.34 minutes 

MG1655: 2707426..2706776
<gbrowseImage> name=NC_000913:2706776..2707426 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2630897..2630247
<gbrowseImage> name=NC_012967:2630247..2630897 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2592581..2593231
<gbrowseImage> name=NC_012759:2592581..2593231 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2708060..2707410
<gbrowseImage> name=NC_007779:2707410..2708060 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2799191..2798541
<gbrowseImage> name=NC_010473:2798541..2799191 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rseA(del) (Keio:JW2556)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10032[3]

rseA::Tn5KAN-I-SceI (FB20857)

Insertion at nt 413 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20857

does not contain pKD46

rseA::Tn5KAN-I-SceI (FB20858)

Insertion at nt 413 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20858

contains pKD46

ΔrseA::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[5]

rseA(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire Glycyl-L-aspartate

PMID:16095938[5]

ΔrseA::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[5]

ΔrseA741::kan

PMID:16738554[2]

CGSC:102808

resA(del)

deletion

Change in the expression of a gene

The expression of rybB is increased in both the Exponential and Stationary phase

PMID:17416652[6]

See Figure 3 A.

CAG45114 rseA(del)::FRTKanFRT

deletion

SigmaE activity

Increase in SigmaE activity

PMID:23224554[7]

Parental Strain: CAG45114 Experimental Strain: SMR15754

See table S11 for full experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2556

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCCAGAAAGAACAACTTTCCGC

Primer 2:CCtTGCGATTGCGTTCCTAAAGT

4A12

Kohara Phage

Genobase

PMID:3038334[9]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[3]

est. P1 cotransduction: 15% [10]
Synonyms:zff-208::Tn10

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[3]

est. P1 cotransduction: 91% [10]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12341

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12341

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002216

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947053

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2245

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008462

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. Thompson, KM et al. (2007) SigmaE regulates and is regulated by a small RNA in Escherichia coli. J. Bacteriol. 189 4243-56 PubMed
  7. Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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