PMID:17416652
| Citation |
Thompson, KM, Rhodius, VA and Gottesman, S (2007) SigmaE regulates and is regulated by a small RNA in Escherichia coli. J. Bacteriol. 189:4243-56 |
|---|---|
| Abstract |
RybB is a small, Hfq-binding noncoding RNA originally identified in a screen of conserved intergenic regions in Escherichia coli. Fusions of the rybB promoter to lacZ were used to screen plasmid genomic libraries and genomic transposon mutants for regulators of rybB expression. A number of plasmids, including some carrying rybB, negatively regulated the fusion. An insertion in the rep helicase and one upstream of dnaK decreased expression of the fusion. Multicopy suppressors of these insertions led to identification of two plasmids that stimulated the fusion. One contained the gene for the response regulator OmpR; the second contained mipA, encoding a murein hydrolase. The involvement of MipA and OmpR in cell surface synthesis suggested that the rybB promoter might be dependent on sigma(E). The sequence upstream of the +1 of rybB contains a consensus sigma(E) promoter. The activity of rybB-lacZ was increased in cells lacking the RseA anti-sigma factor and when sigma(E) was overproduced from a heterologous promoter. The activity of rybB-lacZ and the detection of RybB were totally abolished in an rpoE-null strain. In vitro, sigma(E) efficiently transcribes from this promoter. Both a rybB mutation and an hfq mutation significantly increased expression of both rybB-lacZ and rpoE-lacZ fusions, consistent with negative regulation of the sigma(E) response by RybB and other small RNAs. Based on the plasmid screens, NsrR, a repressor sensitive to nitric oxide, was also found to negatively regulate sigma(E)-dependent promoters in an RseA-independent fashion. |
| Links |
PubMed PMC1913397 Online version:10.1128/JB.00020-07 |
| Keywords |
Base Sequence; Blotting, Northern; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial; Lac Operon/genetics; Molecular Sequence Data; Plasmids/genetics; Promoter Regions, Genetic; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Untranslated/genetics; RNA, Untranslated/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sequence Homology, Nucleic Acid; Sigma Factor/genetics; Sigma Factor/physiology; Transcription, Genetic |
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