rpoB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

rpoB

Gene Synonym(s)

ECK3978, b3987, JW3950, ftsR, groN, nitB, rif, ron, sdgB, stl, stv, tabD[1], mbrD?, tabD, tabG

Product Desc.

RNA polymerase, β subunit[2][3];

Component of RNA polymerase, core enzyme[2][3]; RNA polymerase sigma 24[2][3]; RNA polymerase sigma 38[2][3]; RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]; RNA polymerase sigma54[2]; RNA polymerase sigma 54[3]; RNA polymerase sigma70[2]; RNA polymerase sigma 70[3]; RNA polymerase sigma 19[3]; RNA polymerase sigma 28[3]

RNA polymerase, beta subunit; binds Zn(II)[4]

Product Synonyms(s)

RNA polymerase, beta subunit[1], B3987[2][1], Stl[2][1], Stv[2][1], TabD[2][1], SdgB[2][1], Ron[2][1], Rif[2][1], NitB[2][1], GroN[2][1], FtsR[2][1], RpoB[2][1] , ECK3978, ftsR, groN, JW3950, mbrD?, nitB, rif, ron, sdgB, stl, stv, tabD, tabG, b3987

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rplKAJL-rpoBC[2], rpoBC[2], rplJL-rpoBC[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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HT_Cmplx11_Mem: RpoA+RpoB+RpoC. HT_Cmplx2_Cyt: RpoA+RpoB+RpoC.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpoB

Mnemonic

RNA polymerase

Synonyms

ECK3978, b3987, JW3950, ftsR, groN, nitB, rif, ron, sdgB, stl, stv, tabD[1], mbrD?, tabD, tabG

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90.08 minutes 

MG1655: 4179268..4183296
<gbrowseImage> name=NC_000913:4179268..4183296 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4160856..4164884
<gbrowseImage> name=NC_012967:4160856..4164884 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4068948..4072976
<gbrowseImage> name=NC_012759:4068948..4072976 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3455436..3451408
<gbrowseImage> name=NC_007779:3451408..3455436 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4278964..4282992
<gbrowseImage> name=NC_010473:4278964..4282992 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4179268

Edman degradation

PMID:1095419
PMID:6266829


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rpoBE813K

E813K

Disrupts the enzyme's active center

seeded from UniProt:P0A8V2

rpoB352

CGSC:5386

rpoB308

CGSC:5088

rpoB70

CGSC:5697

rpoB302

CGSC:5724

rpoB301

CGSC:5967

rpoB79

CGSC:6644

rpoB180

CGSC:6647

rpoB318

CGSC:6800

rpoB316

CGSC:7059

rpoB324(rifR)

rifampicin resistant

CGSC:7075

rpoB331(rifR)

rifampicin resistant

CGSC:8722

rpoB306

CGSC:9150

rpoB2(ts)

temperature sensitive

PMID:8113195

CGSC:9774

rpoB7(ts, rifR)

"temperature-sensitive, rifampicin resistant"

CGSC:9775

rpoB3(rifR)

rifampicin resistant

CGSC:10384

rpoB0(rifR)

rifampicin resistant

CGSC:10521

rpoB342

CGSC:10832

rpoB303

CGSC:11987

rpoB23(rifR)

rifampicin resistant

CGSC:14556

rpoB307

CGSC:16669

rpoB317

CGSC:17095

rpoB2066

PMID:7009579

CGSC:67259

rpoB2069

PMID:7009579

CGSC:67261

rpoB2067

PMID:7009579

CGSC:67262

rpoB2068

PMID:7009579

CGSC:67265

rpoB*35

Sensitivity to

decreases sensitivity to bicyclomycin

PMID:21183718

fig. 2; the rpoB*35 allele is described in [5]

rpoB361 ridB1

Dependence

Mutant is dependent on rifampicin. with the condition its grown below 42C in rich medium.

PMID:6757665

strain: PB61

table 3 and figure 1

stv-72

Resistant to

Resistant to Streptovaricin

PMID:4387741

Strain: KY5102

Figure 1.

The paper also describes other alleles with the same phenotype.

mbrD7

Resistant to

Resistance to camphor, other mutants with similar phenotypes are described.

PMID:2176633

PMID:1925019

Table 2 and Table 1

rpoB in strain K100R3

Resistant to

Rfampin resistance

PMID:4879326

Strain: K1100R3

Table 1

rpoB 3445

AA residue affected 507-687

Resistant to

Resistant to rifampicin

PMID:3050121

Several strain were described in table 7

Evidence in table 7

rpoB in strain KY1343

Resistant to

Resistance to Streptolydigin. in vivo.

PMID:4571524

Strains: KY1343, KY1345, KY1360, KY1361, KY1348

Table 2 shows resistance phenotype and other strains with similar mutations.

rpoB in strain PA6075

Resistant to

Resistant to Streptolydigin

PMID:4897821

Strain: PA6075

Figure 1

rpoB706 dnaA1

Growth Phenotype

DnaA1 Mutant is unable to grow at 42.5C, mutation in rpoB suppresses thermosensitivity allowing the strain to grow at 42.5C.

PMID:400782

Experimental Strain: EC8


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3950

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:gccGTTTACTCCTATACCGAGAAAAAACGTATTCG

Primer 2:cctTCGTCTTCCAGTTCGATGTTGATACCC

7B7

Kohara Phage

Genobase

PMID:3038334

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[6]

est. P1 cotransduction: 36% [7]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[6]

est. P1 cotransduction: 59% [7]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10894

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10894

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000885

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948488

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0887

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013041

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RpoB

Synonyms

RNA polymerase, beta subunit[1], B3987[2][1], Stl[2][1], Stv[2][1], TabD[2][1], SdgB[2][1], Ron[2][1], Rif[2][1], NitB[2][1], GroN[2][1], FtsR[2][1], RpoB[2][1] , ECK3978, ftsR, groN, JW3950, mbrD?, nitB, rif, ron, sdgB, stl, stv, tabD, tabG, b3987

Product description

RNA polymerase, β subunit[2][3];

Component of RNA polymerase, core enzyme[2][3]; RNA polymerase sigma 24[2][3]; RNA polymerase sigma 38[2][3]; RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]; RNA polymerase sigma54[2]; RNA polymerase sigma 54[3]; RNA polymerase sigma70[2]; RNA polymerase sigma 70[3]; RNA polymerase sigma 19[3]; RNA polymerase sigma 28[3]

RNA polymerase, beta subunit; binds Zn(II)[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01321

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007120

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007121

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007641

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007642

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007644

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007645

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010243

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01321

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007120

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007121

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007641

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007642

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007644

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007645

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010243

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015712

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019462

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.6

F

Seeded from EcoCyc (v14.0)

complete

GO:0003899

DNA-directed RNA polymerase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0240

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:17960736

IPI: Inferred from Physical Interaction

UniProtKB:P0A8T7

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007120

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007121

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007641

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007642

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007644

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007645

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010243

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015712

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006351

transcription, DNA-dependent

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01321

P

Seeded from EcoCyc (v14.0)

complete

GO:0006351

transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019462

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016779

nucleotidyltransferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0548

F

Seeded from EcoCyc (v14.0)

complete

GO:0032549

ribonucleoside binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015712

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of RNA polymerase, core enzyme

could be indirect

Protein

rplB

PMID:16606699

Experiment(s):EBI-1147178

Protein

ompL

PMID:16606699

Experiment(s):EBI-1147178

Protein

rpoC

PMID:16606699

Experiment(s):EBI-1147178, EBI-1147184

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1147178

Protein

atpD

PMID:15690043

Experiment(s):EBI-879304

Protein

cca

PMID:15690043

Experiment(s):EBI-879304

Protein

cysB

PMID:15690043

Experiment(s):EBI-879304

Protein

hupA

PMID:15690043

Experiment(s):EBI-879304, EBI-883170

Protein

rho

PMID:15690043

Experiment(s):EBI-879304

Protein

rplB

PMID:15690043

Experiment(s):EBI-879304

Protein

rpoA

PMID:15690043

Experiment(s):EBI-879304, EBI-879354, EBI-883170, EBI-883271

Protein

rpoH

PMID:15690043

Experiment(s):EBI-879304, EBI-879426

Protein

rpoN

PMID:15690043

Experiment(s):EBI-879304, EBI-879445, EBI-883645

Protein

rpsC

PMID:15690043

Experiment(s):EBI-879304

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-879304, EBI-883170

Protein

secA

PMID:15690043

Experiment(s):EBI-879304

Protein

tufA

PMID:15690043

Experiment(s):EBI-879304

Protein

yahO

PMID:15690043

Experiment(s):EBI-879304

Protein

ybhF

PMID:15690043

Experiment(s):EBI-879304

Protein

xdhD

PMID:15690043

Experiment(s):EBI-883170

Protein

cycA

PMID:15690043

Experiment(s):EBI-883170

Protein

mutL

PMID:15690043

Experiment(s):EBI-883170

Protein

pstB

PMID:15690043

Experiment(s):EBI-883170

Protein

rhsE

PMID:15690043

Experiment(s):EBI-883170

Protein

ribD

PMID:15690043

Experiment(s):EBI-883170

Protein

rplD

PMID:15690043

Experiment(s):EBI-883170

Protein

rplL

PMID:15690043

Experiment(s):EBI-883170

Protein

rplV

PMID:15690043

Experiment(s):EBI-883170

Protein

rpmG

PMID:15690043

Experiment(s):EBI-883170

Protein

ldcA

PMID:15690043

Experiment(s):EBI-883170

Protein

rnfC

PMID:15690043

Experiment(s):EBI-883170

Protein

rpsE

PMID:15690043

Experiment(s):EBI-883170

Protein

rpsF

PMID:15690043

Experiment(s):EBI-883170

Protein

rpsN

PMID:15690043

Experiment(s):EBI-883170

Protein

rpsT

PMID:15690043

Experiment(s):EBI-883170

Protein

rpsU

PMID:15690043

Experiment(s):EBI-883170

Protein

torR

PMID:15690043

Experiment(s):EBI-883170

Protein

yidE

PMID:15690043

Experiment(s):EBI-883170

Protein

rplK

PMID:15690043

Experiment(s):EBI-883170

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmG

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsT

PMID:19402753

LCMS(ID Probability):99.6

Protein

ribD

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsF

PMID:19402753

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

tufB

PMID:19402753

MALDI(Z-score):21.953813

Protein

secA

PMID:19402753

LCMS(ID Probability):91.9 MALDI(Z-score):17.703872

Protein

rho

PMID:19402753

MALDI(Z-score):24.930275

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):5.643283

Protein

rplB

PMID:19402753

MALDI(Z-score):20.662050

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsU

PMID:19402753

LCMS(ID Probability):99.6

Protein

nusG

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):36.899044

Protein

rpoC

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):14.117317

Protein

rpoS

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):9.798043

Protein

rapA

PMID:19402753

MALDI(Z-score):22.372697

Protein

torR

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

Protein

Subunits of RNA polymerase sigma 24

could be indirect

Protein

Subunits of RNA polymerase sigma 38

could be indirect

Protein

Subunits of RNA polymerase sigma32

could be indirect

Protein

Subunits of RNA polymerase sigma54

could be indirect

Protein

Subunits of RNA polymerase sigma70

could be indirect

Protein

Subunits of RNA polymerase sigma 19

could be indirect

Protein

Subunits of RNA polymerase sigma 28

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MVYSYTEKKR IRKDFGKRPQ VLDVPYLLSI QLDSFQKFIE QDPEGQYGLE AAFRSVFPIQ
SYSGNSELQY VSYRLGEPVF DVQECQIRGV TYSAPLRVKL RLVIYEREAP EGTVKDIKEQ
EVYMGEIPLM TDNGTFVING TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR
GSWLDFEFDP KDNLFVRIDR RRKLPATIIL RALNYTTEQI LDLFFEKVIF EIRDNKLQME
LVPERLRGET ASFDIEANGK VYVEKGRRIT ARHIRQLEKD DVKLIEVPVE YIAGKVVAKD
YIDESTGELI CAANMELSLD LLAKLSQSGH KRIETLFTND LDHGPYISET LRVDPTNDRL
SALVEIYRMM RPGEPPTREA AESLFENLFF SEDRYDLSAV GRMKFNRSLL REEIEGSGIL
SKDDIIDVMK KLIDIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS
LGDLDTLMPQ DMINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGPGGLTR
ERAGFEVRDV HPTHYGRVCP IETPEGPNIG LINSLSVYAQ TNEYGFLETP YRKVTDGVVT
DEIHYLSAIE EGNYVIAQAN SNLDEEGHFV EDLVTCRSKG ESSLFSRDQV DYMDVSTQQV
VSVGASLIPF LEHDDANRAL MGANMQRQAV PTLRADKPLV GTGMERAVAV DSGVTAVAKR
GGVVQYVDAS RIVIKVNEDE MYPGEAGIDI YNLTKYTRSN QNTCINQMPC VSLGEPVERG
DVLADGPSTD LGELALGQNM RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELACVS
RDTKLGPEEI TADIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK
LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQVFTRDGVE KDKRALEIEE MQLKQAKKDL
SEELQILEAG LFSRIRAVLV AGGVEAEKLD KLPRDRWLEL GLTDEEKQNQ LEQLAEQYDE
LKHEFEKKLE AKRRKITQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIN
PIEDMPYDEN GTPVDIVLNP LGVPSRMNIG QILETHLGMA AKGIGDKINA MLKQQQEVAK
LREFIQRAYD LGADVRQKVD LSTFSDEEVM RLAENLRKGM PIATPVFDGA KEAEIKELLK
LGDLPTSGQI RLYDGRTGEQ FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG
GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYKNIVD GNHQMEPGMP
ESFNVLLKEI RSLGINIELE DE
Length

1,342

Mol. Wt

150.635 kDa

pI

5.0 (calculated)

Extinction coefficient

86,070 - 86,945 (calc based on 43 Y, 4 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

513..582

PF04565 RNA polymerase Rpb2, domain 3

PMID:19920124

Domain

715..1264

PF00562 RNA polymerase Rpb2, domain 6

PMID:19920124

Domain

1266..1342

PF04560 RNA polymerase Rpb2, domain 7

PMID:19920124

Domain

26..509

PF04563 RNA polymerase beta subunit

PMID:19920124

Domain

151..277

PF04561 RNA polymerase Rpb2, domain 2

PMID:19920124

Domain

329..454

PF04561 RNA polymerase Rpb2, domain 2

PMID:19920124

Domain

591..656

PF10385 RNA polymerase beta subunit external 1 domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rpoB taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131817

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948488

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013041

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A8V2

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10894

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10894

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948488

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000885

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0887

Escherichia coli str. K-12 substr. MG1655

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<protect></protect>

Notes

Links

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Expression

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

5.59E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

516.058+/-5.925

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.49888+/-0.01658

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.566285998

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

16156

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4059

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

10649

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rplKAJL-rpoBC

rpoBC

rplJL-rpoBC

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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4179248..4179288 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3987 (EcoliWiki Page)

NCBI GEO profiles for rpoB

microarray

GenExpDB:b3987 (EcoliWiki Page)

Summary of data for rpoB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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<protect></protect>

Notes

Accessions Related to rpoB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10894

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0887

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3987

EcoGene

EcoGene:EG10894

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000885

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013041

Escherichia coli str. K-12 substr. MG1655

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<protect></protect>

Notes

Links

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Evolution

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000006850 (score: 1.000; bootstrap: 100%)
  • ENSANGP00000011389 (score: 0.239)
  • ENSANGP00000020031 (score: 0.057)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000035580 (score: 1.000; bootstrap: 99%)
  • ENSAPMP00000023665 (score: 0.204)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G45140 (score: 1.000; bootstrap: 100%)
  • AT4G21710 (score: 0.211)
  • AT3G23780 (score: 0.096)
  • AT3G18090 (score: 0.089)
  • AT1G29940 (score: 0.060)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000025800 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000037862 (score: 0.257)
  • ENSBTAP00000018830 (score: 0.071)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00042252 (score: 1.000; bootstrap: 100%)
  • WBGene00037662 (score: 0.264)
  • WBGene00038678 (score: 0.104)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000003370 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000002728 (score: 0.220)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-041008-1 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-050522-164 (score: 0.624)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0216277 (score: 1.000; bootstrap: 100%)
  • DDB0216313 (score: 0.228)
  • DDB0216278 (score: 0.073)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0003276 (score: 1.000; bootstrap: 100%)
  • FBgn0004463 (score: 0.226)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA16485-PA (score: 1.000; bootstrap: 100%)
  • GA21006-PA (score: 0.228)
  • GA17896-PA (score: 0.050)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000018549 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000020620 (score: 0.231)
  • ENSGALP00000014001 (score: 0.055)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000312735 (score: 1.000; bootstrap: 100%)
  • ENSP00000228347 (score: 0.235)
  • ENSP00000263331 (score: 0.052)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000018408 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000006893 (score: 0.207)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000025899 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000002305 (score: 0.235)

From Inparanoid:20070104

Mus musculus

  • MGI:2388280 (score: 1.000; bootstrap: 100%)
  • MGI:1917678 (score: 0.233)
  • MGI:108014 (score: 0.054)

From Inparanoid:20070104

Oryza gramene

  • P12091 (score: 1.000; bootstrap: 100%)
  • Q6ENI4 (score: 1.000; bootstrap: 100%)
  • Q9XPS7 (score: 1.000; bootstrap: 100%)
  • Q6QXV7 (score: 1.000; bootstrap: 100%)
  • Q7DNA7 (score: 1.000; bootstrap: 100%)
  • Q6QY83 (score: 1.000; bootstrap: 100%)
  • Q7XM77 (score: 1.000; bootstrap: 100%)
  • Q6L3A7 (score: 0.982)
  • Q6ENX3 (score: 0.982)
  • P16023 (score: 0.982)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000027669 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000009150 (score: 0.231)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000030781 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000009746 (score: 0.233)
  • ENSRNOP00000024882 (score: 0.054)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOR207C (score: 1.000; bootstrap: 100%)
  • YOR151C (score: 0.237)
  • YPR010C (score: 0.072)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC4G98c (score: 1.000; bootstrap: 100%)
  • SPAC23G31 (score: 0.233)
  • SPBP23A107 (score: 0.080)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000151234 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000164767 (score: 0.171)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00008589001 (score: 1.000; bootstrap: 100%)
  • GSTENP00022790001 (score: 0.209)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000012617 (score: 1.000; bootstrap: 100%)
  • ENSXETP00000011722 (score: 0.187)
  • ENSXETP00000030114 (score: 0.061)

From Inparanoid:20070104

Shigella flexneri

RPOB

From SHIGELLACYC

E. coli O157

RPOB

From ECOO157CYC

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Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00562 RNA polymerase Rpb2, domain 6

Pfam (EcoliWiki Page)

PF04565 RNA polymerase Rpb2, domain 3

Pfam (EcoliWiki Page)

PF04560 RNA polymerase Rpb2, domain 7

Panther (EcoliWiki Page)

PTHR20856:SF3

Superfamily (EcoliWiki Page)

SUPERFAMILY:64484

Pfam (EcoliWiki Page)

PF04563 RNA polymerase beta subunit

Pfam (EcoliWiki Page)

PF04561 RNA polymerase Rpb2, domain 2

Pfam (EcoliWiki Page)

PF04561 RNA polymerase Rpb2, domain 2

Pfam (EcoliWiki Page)

PF10385 RNA polymerase beta subunit external 1 domain

EcoCyc

EcoCyc:EG10894

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10894

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000885

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0887

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013041

Escherichia coli str. K-12 substr. MG1655

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<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ (could not retreive XML for PMID:xxxx from NCBI. ) could not retreive XML for PMID:xxxx from NCBI. could not retreive XML for PMID:xxxx from NCBI. could not retreive XML for PMID:xxxx from NCBI. could not retreive XML for PMID:xxxx from NCBI. PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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