rpoB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rpoB |
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| Mnemonic |
RNA polymerase |
| Synonyms |
ECK3978, b3987, JW3950, ftsR, groN, nitB, rif, ron, sdgB, stl, stv, tabD[1], mbrD?, tabD, tabG |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
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MG1655 |
90.08 minutes |
MG1655: 4179268..4183296 |
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NC_012967: 4160856..4164884 |
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NC_012759: 4068948..4072976 |
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W3110 |
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W3110: 3455436..3451408 |
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DH10B: 4278964..4282992 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4179268 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
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rpoBE813K |
E813K |
Disrupts the enzyme's active center |
seeded from UniProt:P0A8V2 | ||||
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rpoB352 |
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rpoB308 |
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rpoB70 |
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rpoB302 |
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rpoB301 |
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rpoB79 |
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rpoB180 |
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rpoB318 |
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rpoB316 |
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rpoB324(rifR) |
rifampicin resistant |
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rpoB331(rifR) |
rifampicin resistant |
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rpoB306 |
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rpoB2(ts) |
temperature sensitive |
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rpoB7(ts, rifR) |
"temperature-sensitive, rifampicin resistant" |
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rpoB3(rifR) |
rifampicin resistant |
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rpoB0(rifR) |
rifampicin resistant |
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rpoB342 |
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rpoB303 |
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rpoB23(rifR) |
rifampicin resistant |
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rpoB307 |
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rpoB317 |
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rpoB2066 |
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rpoB2069 |
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rpoB2067 |
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rpoB2068 |
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rpoB*35 |
Sensitivity to |
decreases sensitivity to bicyclomycin |
fig. 2; the rpoB*35 allele is described in [7] | ||||
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rpoB361 ridB1 |
Dependence |
Mutant is dependent on rifampicin. with the condition its grown below 42C in rich medium. |
strain: PB61 |
table 3 and figure 1 | |||
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stv-72 |
Resistant to |
Resistant to Streptovaricin |
Strain: KY5102 |
Figure 1. The paper also describes other alleles with the same phenotype. | |||
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mbrD7 |
Resistant to |
Resistance to camphor, other mutants with similar phenotypes are described. |
Table 2 and Table 1 | ||||
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rpoB in strain K100R3 |
Resistant to |
Rfampin resistance |
Strain: K1100R3 |
Table 1 | |||
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rpoB 3445 |
AA residue affected 507-687 |
Resistant to |
Resistant to rifampicin |
Several strain were described in table 7 |
Evidence in table 7 | ||
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rpoB in strain KY1343 |
Resistant to |
Resistance to Streptolydigin. in vivo. |
Strains: KY1343, KY1345, KY1360, KY1361, KY1348 |
Table 2 shows resistance phenotype and other strains with similar mutations. | |||
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rpoB in strain PA6075 |
Resistant to |
Resistant to Streptolydigin |
Strain: PA6075 |
Figure 1 | |||
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rpoB706 dnaA1 |
Growth Phenotype |
DnaA1 Mutant is unable to grow at 42.5C, mutation in rpoB suppresses thermosensitivity allowing the strain to grow at 42.5C. |
Experimental Strain: EC8 |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3950 |
Plasmid clone |
Status:Clone OK Primer 1:gccGTTTACTCCTATACCGAGAAAAAACGTATTCG Primer 2:cctTCGTCTTCCAGTTCGATGTTGATACCC | |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 36% [20] | ||
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Linked marker |
est. P1 cotransduction: 59% [20] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Fujiki, H & Zurek, G (1975) The subunits of DNA-dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N-terminal regions. FEBS Lett. 55 242-4 PubMed
- ↑ Ovchinnikov, YA et al. (1981) The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit. Eur. J. Biochem. 116 621-9 PubMed
- ↑ Kogoma, T (1994) Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase HI activity. J. Bacteriol. 176 1521-3 PubMed
- ↑ 5.0 5.1 5.2 5.3 Yanofsky, C & Horn, V (1981) Rifampin resistance mutations that alter the efficiency of transcription termination at the tryptophan operon attenuator. J. Bacteriol. 145 1334-41 PubMed
- ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
- ↑ (could not retreive XML for PMID:xxxx from NCBI. ) could not retreive XML for PMID:xxxx from NCBI. could not retreive XML for PMID:xxxx from NCBI. could not retreive XML for PMID:xxxx from NCBI. could not retreive XML for PMID:xxxx from NCBI. PubMed
- ↑ Dabbs, ER (1982) Three additional loci of rifampicin dependence in Escherichia coli. Mol. Gen. Genet. 187 519-22 PubMed
- ↑ Yura, T & Igarashi, K (1968) RNA polymerase mutants of Escherichia coli. I. Mutants resistant to streptovaricin. Proc. Natl. Acad. Sci. U.S.A. 61 1313-9 PubMed
- ↑ Trun, NJ & Gottesman, S (1990) On the bacterial cell cycle: Escherichia coli mutants with altered ploidy. Genes Dev. 4 2036-47 PubMed
- ↑ Trun, NJ & Gottesman, S () Characterization of Escherichia coli mutants with altered ploidy. Res. Microbiol. 142 195-200 PubMed
- ↑ Ezekiel, DH & Hutchins, JE (1968) Mutations affecting RNA polymerase associated with rifampicin resistance in Escherichia coli. Nature 220 276-7 PubMed
- ↑ Jin, DJ & Gross, CA (1988) Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J. Mol. Biol. 202 45-58 PubMed
- ↑ Iwakura, Y et al. (1973) RNA polymerase mutants of Escherichia coli. Streptolydigin resistance and its relation to rifampicin resistance. Mol. Gen. Genet. 121 181-96 PubMed
- ↑ Schleif, R (1969) Isolation and characterization of streptolydigin resistant RNA polymerase. Nature 223 1068-9 PubMed
- ↑ Bagdasarian, MM et al. (1977) Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli. J. Bacteriol. 130 577-82 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 19.0 19.1 CGSC: The Coli Genetics Stock Center
- ↑ 20.0 20.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
