{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	rplL
Gene Synonym(s)	ECK3977, b3986, JW3949^[1], JW3949
Product Desc.	50S ribosomal subunit protein L7/L12^[2]; 50S ribosomal subunit protein L7^[3]; 50S ribosomal subunit protein L12^[3]; Component of 50S ribosomal subunit protein L7/L12 dimer^[3]; 50S ribosomal protein complex L8^[3]; 50S ribosomal subunit^[3]; ribosome^[2]^[3] 50S ribosomal subunit protein L7/L12^[4]
Product Synonyms(s)	50S ribosomal subunit protein L7/L12^[1], B3986^[2]^[1] , ECK3977, JW3949, b3986
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rplL^[2], rplKAJL-rpoBC^[2], rplJL-rpoBC^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	rplL
Mnemonic	Ribosomal protein, large
Synonyms	ECK3977, b3986, JW3949^[1], JW3949
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	90.06 minutes, 90.06 minutes	MG1655: 4178583..4178948 <gbrowseImage> name=NC_000913:4178583..4178948 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4160171..4160536 <gbrowseImage> name=NC_012967:4160171..4160536 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4068263..4068628 <gbrowseImage> name=NC_012759:4068263..4068628 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3456121..3455756 <gbrowseImage> name=NC_007779:3455756..3456121 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4278279..4278644 <gbrowseImage> name=NC_010473:4278279..4278644 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4178586	Edman degradation	PMID:773698 PMID:4573678 PMID:9298646 PMID:9600841 PMID:9868784 PMID:10739205
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3949	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTATCACTAAAGATCAAAT Primer 2:CCTTTAACTTCAACTTCAGCGCC
argE86::Tn10	Linked marker	CAG12185 = CGSC7471^[5]	est. P1 cotransduction: 37% ^[6]
thiC39::Tn10	Linked marker	CAG18500 = CGSC7472^[5]	est. P1 cotransduction: 57% ^[6] Synonyms:thi-39::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10873	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10873	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000864	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948489	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0866	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013038	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RplL
Synonyms	50S ribosomal subunit protein L7/L12^[1], B3986^[2]^[1] , ECK3977, JW3949, b3986
Product description	50S ribosomal subunit protein L7/L12^[2]; 50S ribosomal subunit protein L7^[3]; 50S ribosomal subunit protein L12^[3]; Component of 50S ribosomal subunit protein L7/L12 dimer^[3]; 50S ribosomal protein complex L8^[3]; 50S ribosomal subunit^[3]; ribosome^[2]^[3] 50S ribosomal subunit protein L7/L12^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003735	structural constituent of ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000206	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003735	structural constituent of ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008932	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003735	structural constituent of ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013823	F	Seeded from EcoCyc (v14.0)	complete
	GO:0022625	cytosolic large ribosomal subunit	PMID:4587209	IDA: Inferred from Direct Assay		C	Data in Figure 1.	complete
	GO:0006412	translation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00368	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000206	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008932	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013823	P	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of 50S ribosomal subunit protein L7/L12 dimer	could be indirect
Protein	Subunits of 50S ribosomal protein complex L8	could be indirect
Protein	Subunits of 50S ribosomal subunit	could be indirect
Protein	Subunits of ribosome	could be indirect
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSITKDQIIE AVAAMSVMDV VELISAMEEK FGVSAAAAVA VAAGPVEAAE EKTEFDVILK AAGANKVAVI KAVRGATGLG LKEAKDLVES APAALKEGVS KDDAEALKKA LEEAGAEVEV K
Length	121
Mol. Wt	12.294 kDa
pI	4.5 (calculated)
Extinction coefficient	0 (calc based on 0 Y, 0 W, and 0 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A7K2
Modification Site	90	phosphorylation site at S90	probability greater than 75%	PMID:17938405
Domain	54..121	PF00542 Ribosomal protein L7/L12 C-terminal domain		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rplL taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131816	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948489	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013038	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A7K2	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10873	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10873	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948489	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000864	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0866	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	5.76E+06	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	472965	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	64335	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	154359	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rplL
rplKAJL-rpoBC
rplJL-rpoBC
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:4178563..4178603 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3986 (EcoliWiki Page)	NCBI GEO profiles for rplL
microarray	GenExpDB:b3986 (EcoliWiki Page)	Summary of data for rplL from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4177971..4178213) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ19; Well:G6^[7]
edit table

Notes

Accessions Related to rplL Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10873	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0866	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3986
EcoGene	EcoGene:EG10873	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000864	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013038	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000015113 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G27830 (score: 1.000; bootstrap: 81%) AT3G27850 (score: 1.000; bootstrap: 80%) AT3G27840 (score: 0.606)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000000534 (score: 1.000; bootstrap: 60%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00037769 (score: 1.000; bootstrap: 100%) WBGene00037816 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00012361 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000008691 (score: 1.000; bootstrap: 61%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-050417-187 (score: 1.000; bootstrap: 100%) ZDB-CDNA-050417-188 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0011787 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA18594-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000333837 (score: 1.000; bootstrap: 63%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000003533 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1926273 (score: 1.000; bootstrap: 63%)	From Inparanoid:20070104
Oryza gramene	Q06030 (score: 1.000; bootstrap: 83%) Q06036 (score: 1.000; bootstrap: 82%) Q9ZWF4 (score: 0.629) O22386 (score: 0.515) Q9ZWF5 (score: 0.489)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000016631 (score: 1.000; bootstrap: 62%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YGL068W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC4B39c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000135573 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00019749001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	RPLL	From SHIGELLACYC
E. coli O157	RPLL	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:48300
Panther (EcoliWiki Page)	PTHR11809:SF15
Superfamily (EcoliWiki Page)	SUPERFAMILY:54736
Pfam (EcoliWiki Page)	PF00542 Ribosomal protein L7/L12 C-terminal domain
EcoCyc	EcoCyc:EG10873	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10873	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000864	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0866	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013038	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

rplL:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to rplL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search