rplL:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplL

Mnemonic

Ribosomal protein, large

Synonyms

ECK3977, b3986, JW3949[1], JW3949

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90.06 minutes, 90.06 minutes 

MG1655: 4178583..4178948
<gbrowseImage> name=NC_000913:4178583..4178948 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4160171..4160536
<gbrowseImage> name=NC_012967:4160171..4160536 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4068263..4068628
<gbrowseImage> name=NC_012759:4068263..4068628 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3456121..3455756
<gbrowseImage> name=NC_007779:3455756..3456121 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4278279..4278644
<gbrowseImage> name=NC_010473:4278279..4278644 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4178586

Edman degradation

PMID:773698[2]
PMID:4573678[3]
PMID:9298646[4]
PMID:9600841[5]
PMID:9868784[6]
PMID:10739205[7]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3949

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTCTATCACTAAAGATCAAAT

Primer 2:CCTTTAACTTCAACTTCAGCGCC

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[9]

est. P1 cotransduction: 37% [10]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[9]

est. P1 cotransduction: 57% [10]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10873

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10873

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000864

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948489

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0866

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013038

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Pettersson, I et al. (1976) The ribosomal protein L8 is a complex L7/L12 and L10. FEBS Lett. 64 135-8 PubMed
  3. Terhorst, C et al. (1973) The primary structure of an acidic protein from 50-S ribosomes of Escherichia coli which is involved in GTP hydrolysis dependent on elongation factors G and T. Eur. J. Biochem. 34 138-52 PubMed
  4. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  5. Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  6. Wasinger, VC & Humphery-Smith, I (1998) Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. 169 375-82 PubMed
  7. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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