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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	rplE
Gene Synonym(s)	ECK3295, b3308, JW3270^[1], JW3270
Product Desc.	50S ribosomal subunit protein L5^[2]^[3]; Component of 50S ribosomal subunit^[3]; ribosome^[2]^[3] 50S ribosomal subunit protein L5; 5S rRNA-binding^[4]
Product Synonyms(s)	50S ribosomal subunit protein L5^[1], B3308^[2]^[1], RplE^[2]^[1] , ECK3295, JW3270, b3308
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rplNXE-rpsNH-rplFR-rpsE-rpmD-rplO-secY-rpmJ^[2], rplNXE-rpsNH-rplFR-rpsE-rpmD-rplO-prlA-rpmJ
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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rplE is an essential gene as judged by the inability to recombineer haploid deletions (Baba, 2006; Korepanov, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	rplE
Mnemonic	Ribosomal protein, large
Synonyms	ECK3295, b3308, JW3270^[1], JW3270
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	74.25 minutes, 74.25 minutes	MG1655: 3445460..3444921 <gbrowseImage> name=NC_000913:3444921..3445460 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3375343..3374804 <gbrowseImage> name=NC_012967:3374804..3375343 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3332078..3332617 <gbrowseImage> name=NC_012759:3332078..3332617 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4192978..4193517 <gbrowseImage> name=NC_007779:4192978..4193517 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3543205..3542666 <gbrowseImage> name=NC_010473:3542666..3543205 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3444924	Edman degradation	PMID:791672 PMID:10739205
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
rplE			Resistant to	Thiopeptin-resistant mutants.	PMID:4365100 PMID:1098662	Obtained from E. coli Q 13 by treatment with N-methyl-N'-nitro-N-nitrosoguanidine.	Figure 1 of PMID:4365100
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3270	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCGAAACTGCATGATTACTA Primer 2:CCtTTGCGGAACGGGAAGTCAAA
E1H9	Kohara Phage	Genobase	PMID:3038334
smg-3082::Tn10	Linked marker	CAG12071 = CGSC7444^[5]	est. P1 cotransduction: 56% ^[6] Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 5% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10868	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10868	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000859	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947800	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0861	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010833	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RplE
Synonyms	50S ribosomal subunit protein L5^[1], B3308^[2]^[1], RplE^[2]^[1] , ECK3295, JW3270, b3308
Product description	50S ribosomal subunit protein L5^[2]^[3]; Component of 50S ribosomal subunit^[3]; ribosome^[2]^[3] 50S ribosomal subunit protein L5; 5S rRNA-binding^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000049	tRNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0820	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003723	RNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0694	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003735	structural constituent of ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002132	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003735	structural constituent of ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020929	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003735	structural constituent of ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020930	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005840	ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002132	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005840	ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020929	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005840	ribosome	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020930	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005840	ribosome	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0689	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01333	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002132	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020929	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020930	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019843	rRNA binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01333	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019843	rRNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0699	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030529	ribonucleoprotein complex	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0687	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of 50S ribosomal subunit	could be indirect
Protein	Subunits of ribosome	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKVT LRGERMWEFF ERLITIAVPR IRDFRGLSAK SFDGRGNYSM GVREQIIFPE IDYDKVDRVR GLDITITTTA KSDEEGRALL AAFDFPFRK
Length	179
Mol. Wt	20.302 kDa
pI	9.9 (calculated)
Extinction coefficient	14,440 - 14,565 (calc based on 6 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P62399
Domain	23..80	PF00281 Ribosomal protein L5		PMID:19920124
Domain	84..178	PF00673 ribosomal L5P family C-terminus		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rplE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131187	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947800	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010833	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P62399	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10868	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10868	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947800	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000859	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0861	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.32E+04	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	111791	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	19113	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	63916	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rplNXE-rpsNH-rplFR-rpsE-rpmD-rplO-secY-rpmJ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3445440..3445480 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3308 (EcoliWiki Page)	NCBI GEO profiles for rplE
microarray	GenExpDB:b3308 (EcoliWiki Page)	Summary of data for rplE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to rplE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10868	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0861	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3308
EcoGene	EcoGene:EG10868	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000859	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010833	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000028292 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000014188 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT4G01310 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000027850 (score: 1.000; bootstrap: 100%) ENSBTAP00000033099 (score: 0.399)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00034687 (score: 1.000; bootstrap: 100%) WBGene00024572 (score: 0.914)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00004423 (score: 1.000; bootstrap: 100%) WBGene00004422 (score: 0.917)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000019499 (score: 1.000; bootstrap: 100%) ENSCAFP00000018057 (score: 0.762) ENSCAFP00000001168 (score: 0.749)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000006828 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040625-147 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040625-213 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0214853 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0013325 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000006295 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000270799 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000016049 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1914275 (score: 1.000; bootstrap: 100%) MGI:3644073 (score: 0.940) MGI:3644404 (score: 0.917)	From Inparanoid:20070104
Oryza gramene	Q9ZST0 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000032903 (score: 1.000; bootstrap: 100%) ENSRNOP00000005511 (score: 0.521) ENSRNOP00000034448 (score: 0.459)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDR237W (score: 1.000; bootstrap: 92%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC2F122c (score: 1.000; bootstrap: 82%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000164953 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00003639001 (score: 1.000; bootstrap: 100%) GSTENP00005881001 (score: 0.436)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000002557 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	RPLE	From SHIGELLACYC
E. coli O157	RPLE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR11994:SF4
Superfamily (EcoliWiki Page)	SUPERFAMILY:55282
Pfam (EcoliWiki Page)	PF00281 Ribosomal protein L5
Pfam (EcoliWiki Page)	PF00673 ribosomal L5P family C-terminus
EcoCyc	EcoCyc:EG10868	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10868	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000859	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0861	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010833	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

rplE:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to rplE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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