rplE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rplE |
|---|---|
| Mnemonic |
Ribosomal protein, large |
| Synonyms |
ECK3295, b3308, JW3270[1], JW3270 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
74.25 minutes, 74.25 minutes |
MG1655: 3445460..3444921 |
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NC_012967: 3375343..3374804 |
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NC_012759: 3332078..3332617 |
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W3110 |
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W3110: 4192978..4193517 |
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DH10B: 3543205..3542666 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3444924 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
rplE |
Resistant to |
Thiopeptin-resistant mutants. |
Obtained from E. coli Q 13 by treatment with N-methyl-N'-nitro-N-nitrosoguanidine. |
Figure 1 of PMID:4365100[4] | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3270 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCGAAACTGCATGATTACTA Primer 2:CCtTTGCGGAACGGGAAGTCAAA | |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 56% [9] | ||
|
Linked marker |
est. P1 cotransduction: 5% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Chen, R & Ehrke, G (1976) The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes. FEBS Lett. 69 240-5 PubMed
- ↑ Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
- ↑ 4.0 4.1 Liou, Y et al. (1973) Studies on the ribosomes of a thiopeptin-resistant mutant of Escherichia coli. J. Antibiot. 26 711-6 PubMed
- ↑ Liou, YF et al. (1975) Alteration of ribosomal protein L5 in a thiopeptin-resistant mutant of Escherichia coli. Biochem. Biophys. Res. Commun. 65 1096-101 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
