rho:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

rho

Gene Synonym(s)

ECK3775, b3783, JW3756, nitA, nusD, psuA, rnsC, sun, tsu, psu, sbaA, suA[1], hdf, tabC

Product Desc.

transcription termination factor Rho; polarity suppressor[2][3];

Component of transcription termination factor Rho[3]

Transcription termination factor Rho; bicyclomycin target[4]

Product Synonyms(s)

transcription termination factor[1], B3783[2][1], RnsC[2][1], Tsu[2][1], Sun[2][1], NusD[2][1], Psu[2][1], SbaA[2][1], PsuA[2][1], NitA[2][1], Rho[2][1] , ECK3775, hdf, JW3756, nitA, nusD, psuA, rnsC, sbaA, sun, tabC, tsu, b3783

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rhoL[2], rhoL-rho[2], rho

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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  • The rho gene is often misannotated as a polarity suppressor. Mutations in rho suppress polarity.[5][6][7][8][9] Wild type rho is the source of polarity.
  • rho has been shown to be an essential gene (Das, 1976; Bubunenko, 2007).[4]
  • rho is required to maintain genomic integrity by resolving transcription/replication conflicts [10]

References

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  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Das, A et al. (1976) Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. Proc. Natl. Acad. Sci. U.S.A. 73 1959-63 PubMed
  6. ↑ Korn, LJ & Yanofsky, C (1976) Polarity suppressors defective in transcription termination at the attenuator of the tryptophan operon of Escherichia coli have altered rho factor. J. Mol. Biol. 106 231-41 PubMed
  7. ↑ Belfort, M & Oppenheim, AB (1976) Efficient suppression of the requirement for N function of bacteriophage lambda by a Rho-defective E.coli suA mutant. Mol. Gen. Genet. 148 171-3 PubMed
  8. ↑ Richardson, JP & Carey, JL 3rd (1982) rho Factors from polarity suppressor mutants with defects in their RNA interactions. J. Biol. Chem. 257 5767-71 PubMed
  9. ↑ Das, A et al. (1978) Interaction of RNA polymerase and rho in transcription termination: coupled ATPase. Proc. Natl. Acad. Sci. U.S.A. 75 4828-32 PubMed
  10. ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rho

Mnemonic

Rho termination factor

Synonyms

ECK3775, b3783, JW3756, nitA, nusD, psuA, rnsC, sun, tsu, psu, sbaA, suA[1], hdf, tabC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

85.45 minutes 

MG1655: 3964440..3965699
<gbrowseImage> name=NC_000913:3964440..3965699 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3928217..3929476
<gbrowseImage> name=NC_012967:3928217..3929476 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3854109..3855368
<gbrowseImage> name=NC_012759:3854109..3855368 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3670264..3669005
<gbrowseImage> name=NC_007779:3669005..3670264 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4063360..4064619
<gbrowseImage> name=NC_010473:4063360..4064619 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3964440

Edman degradation

PMID:6219230
PMID:9298646
PMID:9723924


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rhoF64L,A

F64L,A

Defective for RNA-binding

seeded from UniProt:P0AG30

rhoK181Q

K181Q

Partial loss of ATPase, helicase and termination activity

seeded from UniProt:P0AG30

rhoK184Q

K184Q

Improves ATPase and helicase activity but reduced termination activity

seeded from UniProt:P0AG30

rhoF62L,A

F62L,A

Defective for RNA-binding

seeded from UniProt:P0AG30

rhoD265N

D265N

Loss of ATPase activity, helicase and termination activity

seeded from UniProt:P0AG30

rho-15(Ts)

IS1 insertion into C-terminus

changes seven amino acids, eliminates the last two (changes DFFEMMKRS to EVMTPTY)

Growth Phenotype

fails to grow at 42oC, grows slowly at 30oC, suppresses polarity

[2][3]

quickly loses temperature-sensitive phenotype due to accumulation of suppressor mutations.[4]

rho-4

C to A transversion in codon 243

A243E

polarity suppression

suppresses polarity, defective for termination at Rho-dependent terminators

[5][6][7]

CGSC:5339

Originally thought to be an amber (UAG) mutation,[5] but later found to be a missense mutation.[6][7]

rho-112(Am)

CGSC:9184

amber (UAG) mutation

rho-702(ts)

Growth Phenotype

temperature sensitive

CGSC:9191

rho-6

CGSC:38239

rho026 (aka rhoHDF026

Increased degradation of abnormal proteins, such as puromycyl polypeptides or canavanine-containing polypeptides.

PMID:377279

rho026 (aka rhoHDF026)

P103L, S153Y

Unable to support growth of phage T4 and inhibits Lambda phage N activity.

Uninfected cells were temperature-sensitive for growth at 42°C in original isolate.


PMID:6999171 PMID:6225121 PMID:8757797

P103L change is responsible for interfering with T4 plating and Lambda N-mediated antitermination. S153Y was acquired as a suppressor of ts phenotype.

sbaA mutation in SDT1

deletion

Growth Phenotype

deletion of sbaA gene causes limited growth at higher temperatures.

PMID:6247616

See fig 2B for gel electrophoresis results.

sbaA mutation in SDT1

deletion

metabolism

Increase in the accumulation of pppGpp

PMID:6247616

See Fig 3 A & B for full experimental results.

sbaA mutation in SDT1

deletion

beta-galactosidase activity

decrease in the beta-galactosidase activity

PMID:6247616

See Fig 4 for full results.

sbaA mutation in SDT1

deletion

beta-galactosidase activity

addition of 1.0 mM cAMP causes increased beta-galactosidase activity but was significantly lower than parent strain.

PMID:6247616

See Fig 4 for full results.

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Notes

The Keio deletion appears to be the result of a gene duplication, wt rho is still present in the strain.[8]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3756

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAATCTTACCGAATTAAAGAA

Primer 2:CCTGAGCGTTTCATCATTTCGAA

7B1

Kohara Phage

Genobase

PMID:3038334

2A8

Kohara Phage

Genobase

PMID:3038334

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[9]

est. P1 cotransduction: 67% [10]
Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[9]

est. P1 cotransduction: 14% [10]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10845

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10845

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000836

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948297

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0838

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012362

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Rho

Synonyms

transcription termination factor[1], B3783[11][1], RnsC[11][1], Tsu[11][1], Sun[11][1], NusD[11][1], Psu[11][1], SbaA[11][1], PsuA[11][1], NitA[11][1], Rho[11][1] , ECK3775, hdf, JW3756, nitA, nusD, psuA, rnsC, sbaA, sun, tabC, tsu, b3783

Product description

transcription termination factor Rho; polarity suppressor[11][12];

Component of transcription termination factor Rho[12]

Transcription termination factor Rho; bicyclomycin target[13]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011129

F

Seeded from EcoCyc (v14.0)

complete

GO:0003715

transcription termination factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004665

F

Seeded from EcoCyc (v14.0)

complete

GO:0003715

transcription termination factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011112

F

Seeded from EcoCyc (v14.0)

complete

GO:0003715

transcription termination factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011113

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011113

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc (v14.0)

complete

GO:0004386

helicase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0347

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004665

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006353

transcription termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004665

P

Seeded from EcoCyc (v14.0)

complete

GO:0006353

transcription termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011112

P

Seeded from EcoCyc (v14.0)

complete

GO:0006353

transcription termination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011113

P

Seeded from EcoCyc (v14.0)

complete

GO:0006353

transcription termination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0806

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0031555

transcriptional attenuation

PMID:2423505

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of transcription termination factor Rho

could be indirect

Protein

tufA

PMID:15690043

Experiment(s):EBI-884347

Protein

gsp

PMID:15690043

Experiment(s):EBI-891277

Protein

menF

PMID:15690043

Experiment(s):EBI-891277

Protein

rplC

PMID:15690043

Experiment(s):EBI-891277

Protein

rplL

PMID:15690043

Experiment(s):EBI-891277

Protein

rpsB

PMID:15690043

Experiment(s):EBI-891277

Protein

yhbY

PMID:15690043

Experiment(s):EBI-891277

Protein

uspG

PMID:16606699

Experiment(s):EBI-1146678

Protein

yhbY

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):27.463697

Protein

tufB

PMID:19402753

MALDI(Z-score):20.423130

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MNLTELKNTP VSELITLGEN MGLENLARMR KQDIIFAILK QHAKSGEDIF GDGVLEILQD
GFGFLRSADS SYLAGPDDIY VSPSQIRRFN LRTGDTISGK IRPPKEGERY FALLKVNEVN
FDKPENARNK ILFENLTPLH ANSRLRMERG NGSTEDLTAR VLDLASPIGR GQRGLIVAPP
KAGKTMLLQN IAQSIAYNHP DCVLMVLLID ERPEEVTEMQ RLVKGEVVAS TFDEPASRHV
QVAEMVIEKA KRLVEHKKDV IILLDSITRL ARAYNTVVPA SGKVLTGGVD ANALHRPKRF
FGAARNVEEG GSLTIIATAL IDTGSKMDEV IYEEFKGTGN MELHLSRKIA EKRVFPAIDY
NRSGTRKEEL LTTQEELQKM WILRKIIHPM GEIDAMEFLI NKLAMTKTND DFFEMMKRS
Length

419

Mol. Wt

47.005 kDa

pI

7.2 (calculated)

Extinction coefficient

15,930 - 16,055 (calc based on 7 Y, 1 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

157..365

PF00006 ATP synthase alpha/beta family, nucleotide-binding domain

PMID:19920124

Domain

49..126

PF07497 Rho termination factor, RNA-binding domain

PMID:19920124

Domain

5..47

PF07498 Rho termination factor, N-terminal domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rho taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

bicyclomycin

Chemical inhibitor of Rho

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131639

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948297

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012362

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AG30

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10845

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10845

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948297

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000836

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0838

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.45E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

2165.11+/-23.867

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.250595711

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 EMG2

300

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: M68-l

PMID: 9298646

Protein

E. coli K-12 MG1655

17921

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

3478

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

9251

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rhoL

rhoL-rho

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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Rho autoregulates transcription via the Rho attenuator in rhoL [14]

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3964420..3964460 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3783 (EcoliWiki Page)

NCBI GEO profiles for rho

microarray

GenExpDB:b3783 (EcoliWiki Page)

Summary of data for rho from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3963867..3964103) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ08; Well:B10[15]

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Notes

Accessions Related to rho Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10845

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0838

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3783

EcoGene

EcoGene:EG10845

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000836

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012362

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000014693 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000040430 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Shigella flexneri

RHO

From SHIGELLACYC

E. coli O157

RHO

From ECOO157CYC

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00006 ATP synthase alpha/beta family, nucleotide-binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:50249

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Panther (EcoliWiki Page)

PTHR15184:SF2

Superfamily (EcoliWiki Page)

SUPERFAMILY:68912

Pfam (EcoliWiki Page)

PF07497 Rho termination factor, RNA-binding domain

Pfam (EcoliWiki Page)

PF07498 Rho termination factor, N-terminal domain

EcoCyc

EcoCyc:EG10845

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10845

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000836

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0838

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012362

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Das, A et al. (1976) Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. Proc. Natl. Acad. Sci. U.S.A. 73 1959-63 PubMed
  3. ↑ Opperman, T et al. (1995) The ts15 mutation of Escherichia coli alters the sequence of the C-terminal nine residues of Rho protein. Gene 152 133-4 PubMed
  4. ↑ Stewart, V & Yanofsky, C (1985) Evidence for transcription antitermination control of tryptophanase operon expression in Escherichia coli K-12. J. Bacteriol. 164 731-40 PubMed
  5. ↑ 5.0 5.1 Morse, DE & Guertin, M (1972) Amber suA mutations which relieve polarity. J. Mol. Biol. 63 605-8 PubMed
  6. ↑ 6.0 6.1 Ratner, D (1976) Evidence that mutations in the suA polarity suppressing gene directly affect termination factor rho. Nature 259 151-3 PubMed
  7. ↑ 7.0 7.1 Harinarayanan, R & Gowrishankar, J (2003) Host factor titration by chromosomal R-loops as a mechanism for runaway plasmid replication in transcription termination-defective mutants of Escherichia coli. J. Mol. Biol. 332 31-46 PubMed
  8. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  9. ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  11. ↑ 11.00 11.01 11.02 11.03 11.04 11.05 11.06 11.07 11.08 11.09 11.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  12. ↑ 12.0 12.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  13. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  14. ↑ Matsumoto, Y et al. (1986) Autogenous regulation of the gene for transcription termination factor rho in Escherichia coli: localization and function of its attenuators. J. Bacteriol. 166 945-58 PubMed
  15. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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