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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	rbsB
Gene Synonym(s)	ECK3745, b3751, JW3730, prlB, rbsP^[1], rbsP
Product Desc.	RbsB^[2]^[3]; Component of ribose ABC transporter^[2]^[3] D-ribose binding protein, periplasmic; substrate recognition for transport and chemotaxis^[4]
Product Synonyms(s)	D-ribose transporter subunit^[1], periplasmic-binding compoent of ABC superfamily^[1], B3751^[2]^[1], RbsP^[2]^[1], PrlB^[2]^[1], RbsB^[2]^[1] , ECK3745, JW3730, prlB, rbsP, b3751
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rbsDACBKR^[2], OP00289
Regulation/Activity
Quick Links
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Notes

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RbsB binds D-ribose and mediates chemotaxis to D-ribose through the Trg chemoreceptor. Binds TrxA (Kumar, 2004). HT_Cmplx28_Mem: AlsB+RbsB. After four hours of Zn(II) stress, RbsB protein levels decreased (Easton, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	rbsB
Mnemonic	Ribose
Synonyms	ECK3745, b3751, JW3730, prlB, rbsP^[1], rbsP
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	84.8 minutes, 84.8 minutes	MG1655: 3934301..3935191 <gbrowseImage> name=NC_000913:3934301..3935191 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3898085..3898975 <gbrowseImage> name=NC_012967:3898085..3898975 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3822634..3823524 <gbrowseImage> name=NC_012759:3822634..3823524 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3700403..3699513 <gbrowseImage> name=NC_007779:3699513..3700403 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4031885..4032775 <gbrowseImage> name=NC_010473:4031885..4032775 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3934376	Edman degradation	PMID:6313683 PMID:9119048 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔrbsB (Keio:JW3730)	deletion	deletion	PMID:16738554	Shigen CGSC10723^[5]
rbsB::Tn5KAN-I-SceI (FB21436)	Insertion at nt 658 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21436	does not contain pKD46
rbsB::Tn5KAN-I-SceI (FB21437)	Insertion at nt 658 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21437	contains pKD46
rbsB61				CGSC:64031
ΔrbsB780::kan			PMID:16738554	CGSC:102602
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3730	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACATGAAAAAACTGGCTAC Primer 2:CCtTGCTTAACAACCAGTTTCAG
15D10	Kohara Phage	Genobase	PMID:3038334
4F4	Kohara Phage	Genobase	PMID:3038334
rbsD296::Tn10	Linked marker	CAG18501 = CGSC7460^[5]	est. P1 cotransduction: 86% ^[6] Synonyms:zie-296::Tn10
ilvD500::Tn10	Linked marker	CAG18431 = CGSC7462^[5]	est. P1 cotransduction: 51% ^[6] Synonyms:ilv500::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10815	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10815	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000806	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948261	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0808	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012264	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RbsB
Synonyms	D-ribose transporter subunit^[1], periplasmic-binding compoent of ABC superfamily^[1], B3751^[2]^[1], RbsP^[2]^[1], PrlB^[2]^[1], RbsB^[2]^[1] , ECK3745, JW3730, prlB, rbsP, b3751
Product description	RbsB^[2]^[3]; Component of ribose ABC transporter^[2]^[3] D-ribose binding protein, periplasmic; substrate recognition for transport and chemotaxis^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006935	chemotaxis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0145	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008643	carbohydrate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of ribose ABC transporter	could be indirect
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1146638
Protein	nadE		PMID:16606699	Experiment(s):EBI-1146638
Protein	rplD		PMID:16606699	Experiment(s):EBI-1146638
Protein	phnF		PMID:16606699	Experiment(s):EBI-1146638
Protein	livF		PMID:16606699	Experiment(s):EBI-1146638
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Periplasm	PMID:9298646	EchoLocation:rbsB
periplasm		From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNMKKLATLV SAVALSATVS ANAMAKDTIA LVVSTLNNPF FVSLKDGAQK EADKLGYNLV VLDSQNNPAK ELANVQDLTV RGTKILLINP TDSDAVGNAV KMANQANIPV ITLDRQATKG EVVSHIASDN VLGGKIAGDY IAKKAGEGAK VIELQGIAGT SAARERGEGF QQAVAAHKFN VLASQPADFD RIKGLNVMQN LLTAHPDVQA VFAQNDEMAL GALRALQTAG KSDVMVVGFD GTPDGEKAVN DGKLAATIAQ LPDQIGAKGV ETADKVLKGE KVQAKYPVDL KLVVKQ
Length	296
Mol. Wt	30.95 kDa
pI	7.5 (calculated)
Extinction coefficient	4,470 (calc based on 3 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-25	UniProt Manual:Signal Peptides	UniProt:P02925
Domain	26..289	PF00532 family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rbsB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131619	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948261	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012264	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P02925	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10815	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10815	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948261	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000806	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0808	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	4.63E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	0.22+/-0.035	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.199438202	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1714	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	780	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1003	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rbsDACBKR
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3934281..3934321 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3751 (EcoliWiki Page)	NCBI GEO profiles for rbsB
microarray	GenExpDB:b3751 (EcoliWiki Page)	Summary of data for rbsB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to rbsB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10815	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0808	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3751
EcoGene	EcoGene:EG10815	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000806	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012264	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:53822
Pfam (EcoliWiki Page)	PF00532 family
EcoCyc	EcoCyc:EG10815	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10815	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000806	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0808	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012264	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

rbsB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to rbsB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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