rbsA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rbsA

Mnemonic

Ribose

Synonyms

ECK3743, b3749, JW3728, rbsP, rbsT, prlB[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.74 minutes 

MG1655: 3931801..3933306
<gbrowseImage> name=NC_000913:3931801..3933306 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3895585..3897090
<gbrowseImage> name=NC_012967:3895585..3897090 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3820134..3821639
<gbrowseImage> name=NC_012759:3820134..3821639 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3702903..3701398
<gbrowseImage> name=NC_007779:3701398..3702903 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4029385..4030890
<gbrowseImage> name=NC_010473:4029385..4030890 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3931801

Edman degradation

PMID:3086314[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrbsA (Keio:JW3728)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10721[5]

rbsAK43R

K43R

Loss of transport

seeded from UniProt:P04983

rbsD296::Tn10

Insertion at 3,931,872 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG18501 = CGSC7460[5]

Synonyms: zie-296::Tn10

ΔrbsA778::kan

PMID:16738554[4]

CGSC:102600


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3728

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGAAGCATTACTTCAGCTTAA

Primer 2:CCtTCCTGATTCACGCGATTAAG

4F4

Kohara Phage

Genobase

PMID:3038334[8]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[5]

est. P1 cotransduction: 5% [6]

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[5]

est. P1 cotransduction: 46% [6]
Synonyms:ilv500::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10814

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10814

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000805

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948264

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0807

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012259

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Buckel, SD et al. (1986) An analysis of the structure of the product of the rbsA gene of Escherichia coli K12. J. Biol. Chem. 261 7659-62 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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