plsB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

plsB

Gene Synonym(s)

ECK4033, b4041, JW4001[1], JW4001

Product Desc.

GLYCEROL-3-P- Glycerol-3-phosphate acyltransferase[2]

Product Synonyms(s)

glycerol-3-phosphate O-acyltransferase[1], B4041[3][1], PlsB[3][1] , ECK4033, JW4001, b4041

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): plsB[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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HT_Cmplx46_Mem: MdoH+PlsB.[2]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

plsB

Mnemonic

Phospholipid synthesis

Synonyms

ECK4033, b4041, JW4001[1], JW4001

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.65 minutes 

MG1655: 4254489..4252066
<gbrowseImage> name=NC_000913:4252066..4254489 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4235398..4232975
<gbrowseImage> name=NC_012967:4232975..4235398 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4190911..4193334
<gbrowseImage> name=NC_012759:4190911..4193334 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4260056..4257633
<gbrowseImage> name=NC_007779:4257633..4260056 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4354185..4351762
<gbrowseImage> name=NC_010473:4351762..4354185 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4252069

Edman degradation

PMID:6350296


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

plsB26

G1045A[4]

Ala349Thr[4]

Growth Phenotype

Requires glycerol-3-phosphate for growth [5]. However, the block in phospholipid synthesis is only complete in a plsB gspA double mutant.[6]

"The PlsB protein had a significantly reduced glycerol-3-phosphate acyltransferase activity coupled with an elevated Km for glycerol-3-phosphate."[4]

Coli Genetics Stock Center

plsBS308A

S308A

No effect

seeded from UniProt:P0A7A7

plsBD311A

D311A

Prevents assembly into the membrane, suggesting that it paticipates to folding

seeded from UniProt:P0A7A7

plsBD311G

D311G

Strongly reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBH306A

H306A

Abolishes acyltransferase activity

seeded from UniProt:P0A7A7

plsBH306G

H306G

Reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBA349T

A349T

In plsB26; results in high KM for glycerol-3-phosphate and reduced specific activity

seeded from UniProt:P0A7A7

plsBF351A

F351A

Strongly reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBI352A

I352A

Reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBR354C

R354C

Reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBR354K

R354K

No effect

seeded from UniProt:P0A7A7

plsBE385R

E385R

Strongly reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBG386A

G386A

No effect

seeded from UniProt:P0A7A7

plsBG386L

G386L

Reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsBS389A

S389A

No effect

seeded from UniProt:P0A7A7

plsBP421S

P421S

Reduced acyltransferase activity

seeded from UniProt:P0A7A7

plsB13

CGSC:11831

plsB26

PMID:6094487

CGSC:11836

plsB26

G1045A

Ala349Thr

Growth Phenotype

Glycerol-3-phosphate auxotrophs

PMID:10074094

CGSC:11836

GenBankAccession:AF106625.1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4001

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCCGGCTGGCCACGAATTTA

Primer 2:CCaCCTTCGCCCTGCGTCGCACT

1F8

Kohara Phage

Genobase

PMID:3038334

12B4

Kohara Phage

Genobase

PMID:3038334

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[7]

est. P1 cotransduction: 67% [8]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[7]

est. P1 cotransduction: 8% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10740

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10740

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000731

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948541

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0733

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013232

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PlsB

Synonyms

glycerol-3-phosphate O-acyltransferase[1], B4041[3][1], PlsB[3][1] , ECK4033, JW4001, b4041

Product description

GLYCEROL-3-P- Glycerol-3-phosphate acyltransferase[2]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004366

glycerol-3-phosphate O-acyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00393

F

Seeded from EcoCyc (v14.0)

complete

GO:0004366

glycerol-3-phosphate O-acyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.3.1.15

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00393

C

Seeded from EcoCyc (v14.0)

complete

GO:0004366

glycerol-3-phosphate O-acyltransferase activity

PMID:6251087

IMP: Inferred from Mutant Phenotype

F

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0005887

integral to plasma membrane

PMID:6997313

IDA: Inferred from Direct Assay

C

complete

GO:0008654

phospholipid biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00393

P

Seeded from EcoCyc (v14.0)

complete

GO:0008654

phospholipid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0594

P

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

GO:0006631

fatty acid metabolic process

PMID:3542964

IMP: Inferred from Mutant Phenotype

P

complete

GO:0008654

phospholipid biosynthetic process

PMID:3294813

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005887

integral to plasma membrane

PMID:10074094

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

lpdA

PMID:15690043

Experiment(s):EBI-884968

Protein

rho

PMID:15690043

Experiment(s):EBI-884968

Protein

rplC

PMID:15690043

Experiment(s):EBI-884968, EBI-891798

Protein

rplD

PMID:15690043

Experiment(s):EBI-884968

Protein

rpsC

PMID:15690043

Experiment(s):EBI-884968, EBI-891798

Protein

rpsD

PMID:15690043

Experiment(s):EBI-884968, EBI-891798

Protein

srmB

PMID:15690043

Experiment(s):EBI-884968

Protein

tufA

PMID:15690043

Experiment(s):EBI-884968

Protein

rplB

PMID:15690043

Experiment(s):EBI-891798

Protein

rplF

PMID:15690043

Experiment(s):EBI-891798

Protein

rplI

PMID:15690043

Experiment(s):EBI-891798

Protein

rpsE

PMID:15690043

Experiment(s):EBI-891798

Protein

rpsH

PMID:15690043

Experiment(s):EBI-891798

Protein

rpsI

PMID:15690043

Experiment(s):EBI-891798

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-891798

Protein

rluB

PMID:15690043

Experiment(s):EBI-891798

Protein

rplK

PMID:15690043

Experiment(s):EBI-891798

Protein

rplL

PMID:15690043

Experiment(s):EBI-891798

Protein

rplM

PMID:15690043

Experiment(s):EBI-891798

Protein

rplO

PMID:15690043

Experiment(s):EBI-891798

Protein

rplS

PMID:15690043

Experiment(s):EBI-891798

Protein

rplV

PMID:15690043

Experiment(s):EBI-891798

Protein

rplX

PMID:15690043

Experiment(s):EBI-891798

Protein

rpmB

PMID:15690043

Experiment(s):EBI-891798

Protein

rpmG

PMID:15690043

Experiment(s):EBI-891798

Protein

rpsB

PMID:15690043

Experiment(s):EBI-891798

Protein

dnaE

PMID:16606699

Experiment(s):EBI-1147321

Protein

groL

PMID:16606699

Experiment(s):EBI-1147321

Protein

rpsD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rpsC

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

ispA

PMID:19402753

MALDI(Z-score):38.419024

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTFCYPCRAF ALLTRGFTSF MSGWPRIYYK LLNLPLSILV KSKSIPADPA PELGLDTSRP
IMYVLPYNSK ADLLTLRAQC LAHDLPDPLE PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE
SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG RAPGREKGEV NPPLRMLNGV QKFFAVLWLG
RDSFVRFSPS VSLRRMADEH GTDKTIAQKL ARVARMHFAR QRLAAVGPRL PARQDLFNKL
LASRAIAKAV EDEARSKKIS HEKAQQNAIA LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ
GINVHNAERV RQLAHDGHEL VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA
GPIFRRLGAF FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG
TLSMTIQAML RGGTRPITLI PIYIGYEHVM EVGTYAKELR GATKEKESLP QMLRGLSKLR
NLGQGYVNFG EPMPLMTYLN QHVPDWRESI DPIEAVRPAW LTPTVNNIAA DLMVRINNAG
AANAMNLCCT ALLASRQRSL TREQLTEQLN CYLDLMRNVP YSTDSTVPSA SASELIDHAL
QMNKFEVEKD TIGDIIILPR EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH RHISRDVLME
HVNVLYPMLK AELFLRWDRD ELPDVIDALA NEMQRQGLIT LQDDELHINP AHSRTLQLLA
AGARETLQRY AITFWLLSAN PSINRGTLEK ESRTVAQRLS VLHGINAPEF FDKAVFSSLV
LTLRDEGYIS DSGDAEPAET MKVYQLLAEL ITSDVRLTIE SATQGEG
Length

827

Mol. Wt

93.699 kDa

pI

8.7 (calculated)

Extinction coefficient

91,680 - 92,555 (calc based on 32 Y, 8 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A7A7

Domain

281..426

PF01553 Acyltransferase

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=plsB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111678

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948541

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013232

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7A7

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10740

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10740

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948541

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000731

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0733

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.44E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

41.199+/-0.29

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.037144036

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1896

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

445

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

739

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

plsB

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4254469..4254509 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4041 (EcoliWiki Page)

NCBI GEO profiles for plsB

microarray

GenExpDB:b4041 (EcoliWiki Page)

Summary of data for plsB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4254034..4254228) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ09; Well:F4[9]

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Notes

Accessions Related to plsB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10740

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0733

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4041

EcoGene

EcoGene:EG10740

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000731

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013232

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000020227 (score: 1.000; bootstrap: 76%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00037855 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00012911 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000017416 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000018520 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0027579 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA18937-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000018176 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000235553 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000007650 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1343460 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000003562 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000026617 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000156704 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000130206 (score: 0.456)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00030246001 (score: 1.000; bootstrap: 100%)
  • GSTENP00035474001 (score: 0.399)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000005537 (score: 1.000; bootstrap: 100%)
  • ENSXETP00000048781 (score: 0.696)

From Inparanoid:20070104

Shigella flexneri

PLSB

From SHIGELLACYC

E. coli O157

PLSB

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01553 Acyltransferase

Panther (EcoliWiki Page)

PTHR12563:SF3

Superfamily (EcoliWiki Page)

SUPERFAMILY:69593

EcoCyc

EcoCyc:EG10740

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10740

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000731

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0733

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013232

Escherichia coli str. K-12 substr. MG1655

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 Heath, RJ & Rock, CO (1999) A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant. J. Bacteriol. 181 1944-6 PubMed
  5. ↑ Bell, RM (1974) Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J. Bacteriol. 117 1065-76 PubMed
  6. ↑ Ray, TK & Cronan, JE Jr (1987) Acylation of glycerol 3-phosphate is the sole pathway of de novo phospholipid synthesis in Escherichia coli. J. Bacteriol. 169 2896-8 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  9. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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