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Standard Name	panB
Gene Synonym(s)	ECK0133, b0134, JW0130, ts-9^[1], panA
Product Desc.	3-methyl-2-oxobutanoate hydroxymethyltransferase^[2]^[3]; Component of 3-methyl-2-oxobutanoate hydroxymethyltransferase^[2]^[3] Ketopantoate hydroxymethyltransferase^[4]
Product Synonyms(s)	3-methyl-2-oxobutanoate hydroxymethyltransferase^[1], B0134^[2]^[1], PanB^[2]^[1], α-ketoisovalerate hydroxymethyltransferase^[2]^[1], dehydropantoate hydroxymethyltransferase^[2]^[1], ketopantoate hydroxymethyltransferase^[2]^[1], 5,10-methylenetetrahydrofolate:3-methyl-2-oxobutanoate hydroxymethyltransferase^[2]^[1] , ECK0133, JW0130, panA, b0134
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): panBC^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	panB
Mnemonic	Pantothenate
Synonyms	ECK0133, b0134, JW0130, ts-9^[1], panA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	3.21 minutes	MG1655: 149601..148807 <gbrowseImage> name=NC_000913:148807..149601 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 152404..151610 <gbrowseImage> name=NC_012967:151610..152404 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 148806..149600 <gbrowseImage> name=NC_012759:148806..149600 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 149601..148807 <gbrowseImage> name=NC_007779:148807..149601 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 123705..122911 <gbrowseImage> name=NC_010473:122911..123705 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	148807	Edman degradation	PMID:8096212 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
panB(del) (Keio:JW0130)	deletion	deletion	PMID:16738554	Shigen CGSC8406^[5]
panB6				CGSC:6281
panB751(del)::kan			PMID:16738554	CGSC:102246
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0130	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACCGACCACCATCTCCTT Primer 2:CCATGGAAACTGTGTTCTTCGCC
17C11	Kohara Phage	Genobase	PMID:3038334
11C5	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 99% ^[6]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: % ^[6] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11675	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11675	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001618	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944839	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1626	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000467	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PanB
Synonyms	3-methyl-2-oxobutanoate hydroxymethyltransferase^[1], B0134^[2]^[1], PanB^[2]^[1], α-ketoisovalerate hydroxymethyltransferase^[2]^[1], dehydropantoate hydroxymethyltransferase^[2]^[1], ketopantoate hydroxymethyltransferase^[2]^[1], 5,10-methylenetetrahydrofolate:3-methyl-2-oxobutanoate hydroxymethyltransferase^[2]^[1] , ECK0133, JW0130, panA, b0134
Product description	3-methyl-2-oxobutanoate hydroxymethyltransferase^[2]^[3]; Component of 3-methyl-2-oxobutanoate hydroxymethyltransferase^[2]^[3] Ketopantoate hydroxymethyltransferase^[4]
EC number (for enzymes)	2.1.2.11^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003864	3-methyl-2-oxobutanoate hydroxymethyltransferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00156	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003864	3-methyl-2-oxobutanoate hydroxymethyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003700	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003864	3-methyl-2-oxobutanoate hydroxymethyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.1.2.11	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of 3-methyl-2-oxobutanoate hydroxymethyltransferase	could be indirect
Protein	ileS		PMID:16606699	Experiment(s):EBI-1135688
Protein	pflA		PMID:16606699	Experiment(s):EBI-1135688
Protein	rpsD		PMID:16606699	Experiment(s):EBI-1135688
Protein	iscS		PMID:16606699	Experiment(s):EBI-1135688
Protein	carA		PMID:16606699	Experiment(s):EBI-1135688
Protein	slyD		PMID:16606699	Experiment(s):EBI-1135688
Protein	tig		PMID:19402753	LCMS(ID Probability):99.6
Protein	rpmC		PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsT		PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsJ		PMID:19402753	LCMS(ID Probability):99.6
Protein	cspC		PMID:19402753	LCMS(ID Probability):99.6
Protein	pgm		PMID:19402753	LCMS(ID Probability):99.6
Protein	ppsA		PMID:19402753	LCMS(ID Probability):99.6
Protein	talB		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKPTTISLLQ KYKQEKKRFA TITAYDYSFA KLFADEGLNV MLVGDSLGMT VQGHDSTLPV TVADIAYHTA AVRRGAPNCL LLADLPFMAY ATPEQAFENA ATVMRAGANM VKIEGGEWLV ETVQMLTERA VPVCGHLGLT PQSVNIFGGY KVQGRGDEAG DQLLSDALAL EAAGAQLLVL ECVPVELAKR ITEALAIPVI GIGAGNVTDG QILVMHDAFG ITGGHIPKFA KNFLAETGDI RAAVRQYMAE VESGVYPGEE HSFH
Length	264
Mol. Wt	28.236 kDa
pI	5.1 (calculated)
Extinction coefficient	17,420 - 17,795 (calc based on 8 Y, 1 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	2..260	PF02548 Ketopantoate hydroxymethyltransferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=panB taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128127	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944839	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000467	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31057	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11675	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11675	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944839	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001618	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1626	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.11E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	7878	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2859	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	5421	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
panBC
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:149581..149621 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0134 (EcoliWiki Page)	NCBI GEO profiles for panB
microarray	GenExpDB:b0134 (EcoliWiki Page)	Summary of data for panB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (149527..149746) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ17; Well:E1^[7]
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Notes

Accessions Related to panB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11675	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1626	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0134
EcoGene	EcoGene:EG11675	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001618	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000467	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G46110 (score: 1.000; bootstrap: 100%) AT3G61530 (score: 0.851)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0231509 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YBR176W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC5H109c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PANB	From SHIGELLACYC
E. coli O157	PANB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:51621
Panther (EcoliWiki Page)	PTHR20881:SF0
Pfam (EcoliWiki Page)	PF02548 Ketopantoate hydroxymethyltransferase
EcoCyc	EcoCyc:EG11675	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11675	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001618	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1626	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000467	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

panB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to panB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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