panB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

panB

Mnemonic

Pantothenate

Synonyms

ECK0133, b0134, JW0130, ts-9[1], panA

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

3.21 minutes 

MG1655: 149601..148807
<gbrowseImage> name=NC_000913:148807..149601 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 152404..151610
<gbrowseImage> name=NC_012967:151610..152404 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 148806..149600
<gbrowseImage> name=NC_012759:148806..149600 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 149601..148807
<gbrowseImage> name=NC_007779:148807..149601 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 123705..122911
<gbrowseImage> name=NC_010473:122911..123705 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

148807

Edman degradation

PMID:8096212[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

panB(del) (Keio:JW0130)

deletion

deletion

PMID:16738554[4]

Shigen

CGSC8406[5]

panB6

CGSC:6281

panB751(del)::kan

PMID:16738554[4]

CGSC:102246


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0130

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAACCGACCACCATCTCCTT

Primer 2:CCATGGAAACTGTGTTCTTCGCC

17C11

Kohara Phage

Genobase

PMID:3038334[7]

11C5

Kohara Phage

Genobase

PMID:3038334[7]

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 99% [8]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: % [8]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11675

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11675

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001618

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944839

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1626

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000467

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Jones, CE et al. (1993) Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme. J. Bacteriol. 175 2125-30 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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