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Gene |

Product(s) |

Expression|

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Standard Name	otsB
Gene Synonym(s)	ECK1896, b1897, JW1886, otsP^[1], otsP
Product Desc.	Trehalose phosphate phosphatase; cold- and heat- induced; required for viability at 4C; rpoS regulon; HAD17^[2]
Product Synonyms(s)	trehalose-6-phosphate phosphatase, biosynthetic^[1], B1897^[3]^[1], OtsP^[3]^[1], OtsB^[3]^[1] , ECK1896, JW1886, otsP, b1897
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): otsBA^[3]
Regulation/Activity
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Notes

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HAD superfamily.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	otsB
Mnemonic	Osmoregulated trehalose synthesis
Synonyms	ECK1896, b1897, JW1886, otsP^[1], otsP
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	42.67 minutes	MG1655: 1980411..1979611 <gbrowseImage> name=NC_000913:1979611..1980411 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1960381..1959581 <gbrowseImage> name=NC_012967:1959581..1960381 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1872094..1872894 <gbrowseImage> name=NC_012759:1872094..1872894 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1984524..1983724 <gbrowseImage> name=NC_007779:1983724..1984524 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2071419..2070619 <gbrowseImage> name=NC_010473:2070619..2071419 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1979611	Edman degradation	PMID:8045430
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔotsB (Keio:JW1886)	deletion	deletion	PMID:16738554	Shigen CGSC9568^[4]
otsB::Tn5KAN-2 (FB20504)	Insertion at nt 246 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20504	contains pKD46
otsB::Tn5KAN-2 (FB20505)	Insertion at nt 246 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20505	does not contain pKD46
ΔotsB748::kan			PMID:16738554	CGSC:102210
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1886	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACAGAACCGTTAACCGAAAC Primer 2:CCGATACTACGACTAAACGACTC
7F11	Kohara Phage	Genobase	PMID:3038334
18A9	Kohara Phage	Genobase	PMID:3038334
eda-51::Tn10	Linked marker	CAG18486 = CGSC7392^[4]	est. P1 cotransduction: 10% ^[5]
uvrC279::Tn10	Linked marker	CAG12156 = CGSC7394^[4]	est. P1 cotransduction: 69% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11752	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11752	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001690	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946406	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1702	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006320	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	OtsB
Synonyms	trehalose-6-phosphate phosphatase, biosynthetic^[1], B1897^[3]^[1], OtsP^[3]^[1], OtsB^[3]^[1] , ECK1896, JW1886, otsP, b1897
Product description	Trehalose phosphate phosphatase; cold- and heat- induced; required for viability at 4C; rpoS regulon; HAD17^[2]
EC number (for enzymes)	3.1.3.12^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003337	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006006	glucose metabolic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006379	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004805	trehalose-phosphatase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.12	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005992	trehalose biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003337	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006379	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dadA	PMID:16606699	Experiment(s):EBI-1141241
Protein	nadE	PMID:16606699	Experiment(s):EBI-1141241
Protein	gadW	PMID:16606699	Experiment(s):EBI-1141241
Protein	rho	PMID:16606699	Experiment(s):EBI-1141241
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTEPLTETPE LSAKYAWFFD LDGTLAEIKP HPDQVVVPDN ILQGLQLLAT ASDGALALIS GRSMVELDAL AKPYRFPLAG VHGAERRDIN GKTHIVHLPD AIARDISVQL HTVIAQYPGA ELEAKGMAFA LHYRQAPQHE DALMTLAQRI TQIWPQMALQ QGKCVVEIKP RGTSKGEAIA AFMQEAPFIG RTPVFLGDDL TDESGFAVVN RLGGMSVKIG TGATQASWRL AGVPDVWSWL EMITTALQQK RENNRSDDYE SFSRSI
Length	266
Mol. Wt	29.175 kDa
pI	5.4 (calculated)
Extinction coefficient	34,950 - 35,075 (calc based on 5 Y, 5 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	18..234	PF02358 Trehalose-phosphatase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=otsB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129849	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946406	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006320	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31678	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11752	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11752	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946406	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001690	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1702	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	201	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
otsBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1980391..1980431 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1897 (EcoliWiki Page)	NCBI GEO profiles for otsB
microarray	GenExpDB:b1897 (EcoliWiki Page)	Summary of data for otsB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1980318..1980578) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ11; Well:C4^[6]
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Notes

Accessions Related to otsB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11752	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1702	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1897
EcoGene	EcoGene:EG11752	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001690	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006320	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G78090 (score: 1.000; bootstrap: 100%) AT1G35910 (score: 0.550) AT1G22210 (score: 0.539) AT5G65140 (score: 0.532) AT5G10100 (score: 0.478) AT4G39770 (score: 0.477) AT2G22190 (score: 0.465) AT5G51460 (score: 0.404) AT4G12430 (score: 0.402) AT4G22590 (score: 0.402)	From Inparanoid:20070104
Oryza gramene	Q6ZAL2 (score: 1.000; bootstrap: 100%) Q6H5L4 (score: 0.677) Q6ZGP8 (score: 0.565) Q67VY4 (score: 0.486) Q7XI41 (score: 0.451) Q75WV3 (score: 0.448) Q6AVE7 (score: 0.438) Q9FWQ2 (score: 0.411) Q7XT34 (score: 0.377) Q9XFD7 (score: 0.117)	From Inparanoid:20070104
Shigella flexneri	OTSB	From SHIGELLACYC
E. coli O157	OTSB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR10788:SF6
Superfamily (EcoliWiki Page)	SUPERFAMILY:56784
Pfam (EcoliWiki Page)	PF02358 Trehalose-phosphatase
EcoCyc	EcoCyc:EG11752	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11752	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001690	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1702	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006320	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

otsB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to otsB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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