nuoG:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nuoG |
|---|---|
| Mnemonic |
NADH:ubiquinone oxidoreductase |
| Synonyms |
ECK2277, b2283, JW2278[1], JW2278 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
51.63 minutes |
MG1655: 2398193..2395461 |
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NC_012967: 2343762..2341030 |
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NC_012759: 2281266..2283992 |
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W3110 |
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W3110: 2404841..2402109 |
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DH10B: 2489958..2487226 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2395464 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
nuoG(del) (Keio:JW2278) |
deletion |
deletion |
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nuoG::Tn5KAN-2 (FB20742) |
Insertion at nt 519 in Minus orientation |
does not contain pKD46 | |||||
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nuoG::Tn5KAN-2 (FB20743) |
Insertion at nt 519 in Minus orientation |
contains pKD46 | |||||
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire D-Galactose |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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|
nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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|
nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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|
nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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|
nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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nuoG(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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nuoG765(del)::kan |
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nuoG::Tn10dCam |
Insertion |
Mutagenesis Rate |
Decrease in stress induced mutagenesis (SIM). |
Parent Strain: SMR4562 Experimental Strain: SMR13640 |
The Mutation conferred a strong decrease in SIM with mutant frequency being decreased by over 90 percent. See Table S3 for full experimental data. | ||
|
nuoG::Tn10dCam |
Insertion |
SDS-EDTA Sensitivity |
Parent Strain: SMR4562 Experimental Strain: SMR13640 |
The Mutation conferred an increase in SDS-EDTA sensitivity. See table S7 and S1 for a summary of experimental results. | |||
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SMR4562 yiaG-yfp FRT nuoG::Tn10dCam |
insertion |
SigmaS activity |
Decrease in sigmaS activity |
Parental Strain: SMR10582 Experimental Strain: SMR14420 |
See table S8 for full experimental results. | ||
|
CAG45114 nuoG::Tn10dCam |
Insertion |
SigmaE activity |
Decrease in SigmaE activity |
Parental Strain: CAG45114 Experimental Strain: SMR16038 |
See table S11 for experimental results. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2278 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCTAATGGCTACAATTCATGT Primer 2:CCTTGTTGTGCCTCCTTGAGATC | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 82% [11] | ||
|
zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: % [11] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Moreno-Bruna, B et al. (2001) Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 98 8128-32 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ 8.0 8.1 8.2 8.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 10.0 10.1 10.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
