nuoG:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NuoG

Synonyms

NADH:ubiquinone oxidoreductase, chain G[1], B2283[2][1], NuoG[2][1] , ECK2277, JW2278, b2283

Product description

NuoG[2][3];

Component of soluble NADH dehydrogenase fragment[3]; NADH dehydrogenase I[2][3]

NADH:ubiquinone oxidoreductase subunit G, complex I; NADH dehydrogenase I[4]

EC number (for enzymes) 
1.6.5.3[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

NOT

GO:0005737

cytoplasm

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

See discussion page for explanation of 'NOT' qualifier.

complete

GO:0030964

NADH dehydrogenase complex

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

The E. coli NADH dehydrogenase complex was purified and the subunits separated by SDS-PAGE. The N-termini of these 6 polypeptides were sequenced after deblocking with methanolic HCl.

complete

GO:0005886

plasma membrane

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

The NADH dehydrogenase I complex fractionates with cytoplasmic membranes.

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0003954

NADH dehydrogenase activity

PMID:3122832[6]

IDA: Inferred from Direct Assay

F

Purified NADH dehydrogenase I in membrane vesicles can oxidize NADH.

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

PMID:17640900[7]

IDA: Inferred from Direct Assay

F

EPR spectra from purified NuoG reduced by dithionite gave evidence of two rhombic [4Fe-4S] clusters (Fig. 2 and text).

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0051537

2 iron, 2 sulfur cluster binding

PMID:17640900[7]

IDA: Inferred from Direct Assay

F

EPR spectra from purified NuoG reduced by dithionite gave evidence of an axial [2Fe-2S] cluster (Fig. 2 and text).

complete

GO:0008137

NADH dehydrogenase (ubiquinone) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000283

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0010181

FMN binding

PMID:7607227[5]

IDA: Inferred from Direct Assay

F

The water-soluble NADH fragment contains the subunits NuoE, NuoF, and NuoG. This fragment also contains FMN and has the EPR-detectable FeS clusters N1b, N1c, N3, and N4.

complete

GO:0009055

electron carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001041

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006656

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006657

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006963

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0016651

oxidoreductase activity, acting on NADH or NADPH

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010228

F

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006657

F

Seeded from EcoCyc (v14.0)

complete

GO:0042773

ATP synthesis coupled electron transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000283

P

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0048038

quinone binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0874

F

Seeded from EcoCyc (v14.0)

complete

GO:0050136

NADH dehydrogenase (quinone) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.6.99.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0051537

2 iron, 2 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0001

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000283

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010228

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of soluble NADH dehydrogenase fragment

could be indirect

Protein

skp

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

leuD

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

nadC

PMID:19402753[8]

LCMS(ID Probability):99.3

Protein

purA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

purL

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

nuoF

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

nuoE

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

Subunits of NADH dehydrogenase I

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytoplasm

PMID:9298646[9], PMID:11416161[10]

EchoLocation:nuoG


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLMATIHVDG KEYEVNGADN LLEACLSLGL DIPYFCWHPA LGSVGACRQC AVKQYQNAED
TRGRLVMSCM TPASDGTFIS IDDEEAKQFR ESVVEWLMTN HPHDCPVCEE GGNCHLQDMT
VMTGHSFRRY RFTKRTHRNQ DLGPFISHEM NRCIACYRCV RYYKDYADGT DLGVYGAHDN
VYFGRPEDGT LESEFSGNLV EICPTGVFTD KTHSERYNRK WDMQFAPSIC QQCSIGCNIS
PGERYGELRR IENRYNGTVN HYFLCDRGRF GYGYVNLKDR PRQPVQRRGD DFITLNAEQA
MQGAADILRQ SKKVIGIGSP RASVESNFAL RELVGEENFY TGIAHGEQER LQLALKVLRE
GGIYTPALRE IESYDAVLVL GEDVTQTGAR VALAVRQAVK GKAREMAAAQ KVADWQIAAI
LNIGQRAKHP LFVTNVDDTR LDDIAAWTYR APVEDQARLG FAIAHALDNS APAVDGIEPE
LQSKIDVIVQ ALAGAKKPLI ISGTNAGSLE VIQAAANVAK ALKGRGADVG ITMIARSVNS
MGLGIMGGGS LEEALTELET GRADAVVVLE NDLHRHASAI RVNAALAKAP LVMVVDHQRT
AIMENAHLVL SAASFAESDG TVINNEGRAQ RFFQVYDPAY YDSKTVMLES WRWLHSLHST
LLSREVDWTQ LDHVIDAVVA KIPELAGIKD AAPDATFRIR GQKLAREPHR YSGRTAMRAN
ISVHEPRQPQ DIDTMFTFSM EGNNQPTAHR SQVPFAWAPG WNSPQAWNKF QDEVGGKLRF
GDPGVRLFET SENGLDYFTS VPARFQPQDG KWRIAPYYHL FGSDELSQRA PVFQSRMPQP
YIKLNPADAA KLGVNAGTRV SFSYDGNTVT LPVEIAEGLT AGQVGLPMGM SGIAPVLAGA
HLEDLKEAQQ
Length

910

Mol. Wt

100.543 kDa

pI

6.2 (calculated)

Extinction coefficient

109,210 - 111,210 (calc based on 29 Y, 12 W, and 16 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P33602

Domain

4..72

PF00111 2Fe-2S iron-sulfur cluster binding domain

PMID:19920124[11]

Domain

221..275

PF04879 Molybdopterin oxidoreductase Fe4S4 domain

PMID:19920124[11]

Domain

510..643

PF00384 Molybdopterin oxidoreductase

PMID:19920124[11]

Domain

821..904

PF01568 Molydopterin dinucleotide binding domain

PMID:19920124[11]

Domain

88..128

PF10588 NADH-ubiquinone oxidoreductase-G iron-sulfur binding region

PMID:19920124[11]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nuoG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:145698290

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946762

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007543

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P33602

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12087

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12087

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946762

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001992

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2011

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Leif, H et al. (1995) Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli. Eur. J. Biochem. 230 538-48 PubMed
  6. ↑ Matsushita, K et al. (1987) NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Biochemistry 26 7732-7 PubMed
  7. ↑ 7.0 7.1 Yakovlev, G et al. (2007) Reevaluating the relationship between EPR spectra and enzyme structure for the iron sulfur clusters in NADH:quinone oxidoreductase. Proc. Natl. Acad. Sci. U.S.A. 104 12720-5 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  10. ↑ Moreno-Bruna, B et al. (2001) Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 98 8128-32 PubMed
  11. ↑ 11.0 11.1 11.2 11.3 11.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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