nuoF:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nuoF |
|---|---|
| Mnemonic |
NADH:ubiquinone oxidoreductase |
| Synonyms |
ECK2278, b2284, JW2279[1], JW2279, nuoB |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
51.69 minutes |
MG1655: 2399577..2398240 |
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NC_012967: 2345146..2343809 |
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NC_012759: 2284045..2285382 |
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W3110 |
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W3110: 2406225..2404888 |
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DH10B: 2491342..2490005 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2398240 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔnuoF (Keio:JW2279) |
deletion |
deletion |
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nuoF::Tn5KAN-2 (FB20744) |
Insertion at nt 642 in Plus orientation |
contains pKD46 | |||||
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nuoF::Tn5KAN-2 (FB20745) |
Insertion at nt 642 in Plus orientation |
does not contain pKD46 | |||||
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire D-Galactose |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire DL-a-Glycerol |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire DL-Lactate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Glycyl-L-aspartate |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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|
ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Inosine |
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ΔnuoF::kan |
deletion |
Biolog:respiration |
unable to respire Uridine |
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|
ΔnuoF766::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2279 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAAACATTATCCGTACTCC Primer 2:CCCCAGCGCTCTTTCAGCAGGTT | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 74% [10] | ||
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zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: % [10] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Moreno-Bruna, B et al. (2001) Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 98 8128-32 PubMed
- ↑ David, P et al. (2002) Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues. Biochim. Biophys. Acta 1553 268-78 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
