nuoF:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NuoF

Synonyms

NADH:ubiquinone oxidoreductase, chain F[1], B2284[2][1], NuoF[2][1] , ECK2278, JW2279, nuoB, b2284

Product description

NuoF[2][3];

Component of soluble NADH dehydrogenase fragment[3]; NADH dehydrogenase I[2][3]

NADH:ubiquinone oxidoreductase subunit F, complex I; NADH dehydrogenase I[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

NOT

GO:0005737

cytoplasm

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

See discussion page for explanation of 'NOT' qualifier.

complete

GO:0005886

plasma membrane

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

The NADH dehydrogenase I complex fractionates with cytoplasmic membranes.

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0003954

NADH dehydrogenase activity

PMID:3122832[6]

IDA: Inferred from Direct Assay

F

Purified NADH dehydrogenase I in membrane vesicles can oxidize NADH.

complete

GO:0008137

NADH dehydrogenase (ubiquinone) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001949

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

PMID:15922336[7]

IMP: Inferred from Mutant Phenotype

F

Mutation of cysteine residues C351, C354, C357, or C398 eliminates the N3 4Fe-4S cluster in NuoF.

complete

GO:0010181

FMN binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001949

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0010181

FMN binding

PMID:7607227[5]

IDA: Inferred from Direct Assay

F

The water-soluble NADH fragment contains the subunits NuoE, NuoF, and NuoG. This fragment also contains FMN and has the EPR-detectable FeS clusters N1b, N1c, N3, and N4.

complete

GO:0010181

FMN binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011537

F

Seeded from EcoCyc (v14.0)

complete

GO:0030964

NADH dehydrogenase complex

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

The E. coli NADH dehydrogenase complex was purified and the subunits separated by SDS-PAGE. The N-termini of these 6 polypeptides were sequenced after deblocking with methanolic HCl.

complete

GO:0010181

FMN binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011538

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0016651

oxidoreductase activity, acting on NADH or NADPH

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011537

F

Seeded from EcoCyc (v14.0)

complete

GO:0016651

oxidoreductase activity, acting on NADH or NADPH

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011538

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0048038

quinone binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0874

F

Seeded from EcoCyc (v14.0)

complete

GO:0050136

NADH dehydrogenase (quinone) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.6.99.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011537

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011538

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001949

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011537

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011538

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001949

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011537

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of soluble NADH dehydrogenase fragment

could be indirect

Protein

nuoE

PMID:16606699[8]

Experiment(s):EBI-1142426, EBI-1142433

Protein

fadB

PMID:16606699[8]

Experiment(s):EBI-1142426

Protein

nuoG

PMID:16606699[8]

Experiment(s):EBI-1142426

Protein

uvrB

PMID:16606699[8]

Experiment(s):EBI-1142426

Protein

groL

PMID:16606699[8]

Experiment(s):EBI-1142426

Protein

Subunits of NADH dehydrogenase I

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKNIIRTPET HPLTWRLRDD KQPVWLDEYR SKNGYEGARK ALTGLSPDEI VNQVKDAGLK
GRGGAGFSTG LKWSLMPKDE SMNIRYLLCN ADEMEPGTYK DRLLMEQLPH LLVEGMLISA
FALKAYRGYI FLRGEYIEAA VNLRRAIAEA TEAGLLGKNI MGTGFDFELF VHTGAGRYIC
GEETALINSL EGRRANPRSK PPFPATSGAW GKPTCVNNVE TLCNVPAILA NGVEWYQNIS
KSKDAGTKLM GFSGRVKNPG LWELPFGTTA REILEDYAGG MRDGLKFKAW QPGGAGTDFL
TEAHLDLPME FESIGKAGSR LGTALAMAVD HEINMVSLVR NLEEFFARES CGWCTPCRDG
LPWSVKILRA LERGEGQPGD IETLEQLCRF LGPGKTFCAH APGAVEPLQS AIKYFREEFE
AGIKQPFSNT HLINGIQPNL LKERW
Length

445

Mol. Wt

49.293 kDa

pI

6.8 (calculated)

Extinction coefficient

71,390 - 72,515 (calc based on 11 Y, 10 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

54..227

PF01512 Respiratory-chain NADH dehydrogenase 51 Kd subunit

PMID:19920124[9]

Domain

252..300

PF10531 SLBB domain

PMID:19920124[9]

Domain

336..381

PF10589 NADH-ubiquinone oxidoreductase-F iron-sulfur binding region

PMID:19920124[9]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nuoF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130219

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946753

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007545

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P31979

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11774

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11774

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946753

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001708

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1723

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Leif, H et al. (1995) Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli. Eur. J. Biochem. 230 538-48 PubMed
  6. ↑ Matsushita, K et al. (1987) NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Biochemistry 26 7732-7 PubMed
  7. ↑ Velazquez, I et al. (2005) Amino acid residues associated with cluster N3 in the NuoF subunit of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli. FEBS Lett. 579 3164-8 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ 9.0 9.1 9.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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