nfi:On One Page
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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| Standard Name |
nfi |
|---|---|
| Gene Synonym(s) | |
| Product Desc. |
endonuclease V (deoxyinosine 3'endoduclease)[2][3] Endonuclease V, specific for single-stranded DNA or duplex DNA with damaged U[4] |
| Product Synonyms(s) |
endonuclease V[1], B3998[2][1], YjaF[2][1], YfaF[2][1], Nfi[2][1], EndoV[2][1] , ECK3990, JW5547, yjaF, b3998 |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links | |
| edit table |
See Help:Quickview for help with entering information in the Quickview table.
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nfi |
|---|---|
| Mnemonic |
Endonuclease V |
| Synonyms | |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
90.45 minutes, 90.45 minutes |
MG1655: 4196813..4197484 |
||
|
NC_012967: 4178402..4179073 |
||||
|
NC_012759: 4086493..4087164 |
||||
|
W3110 |
|
W3110: 3437891..3437220 |
||
|
DH10B: 4296509..4297180 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4196813 |
Edman degradation |
PMID:8990280 |
| |
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Δnfi (Keio:JW5547) |
deletion |
deletion |
PMID:16738554 |
||||
|
Δnfi-769::kan |
PMID:16738554 |
| |||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW5547 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGATCTCGCGTCATTACGCGC Primer 2:CCGGGCTGATTTGCTGTATAGCG | |
|
Kohara Phage |
PMID:3038334 | ||
|
Linked marker |
est. P1 cotransduction: 92% [6] | ||
|
Linked marker |
est. P1 cotransduction: 15% [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120001843 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EchoBASE:EB1859 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0013068 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Nfi |
|---|---|
| Synonyms |
endonuclease V[1], B3998[2][1], YjaF[2][1], YfaF[2][1], Nfi[2][1], EndoV[2][1] , ECK3990, JW5547, yjaF, b3998 |
| Product description |
endonuclease V (deoxyinosine 3'endoduclease)[2][3] Endonuclease V, specific for single-stranded DNA or duplex DNA with damaged U[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000287 |
magnesium ion binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000287 |
magnesium ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004518 |
nuclease activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004519 |
endonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004519 |
endonuclease activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006974 |
response to DNA damage stimulus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016787 |
hydrolase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016788 |
hydrolase activity, acting on ester bonds |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0043737 |
deoxyribonuclease V activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
aceF |
Experiment(s):EBI-888807, EBI-893916 | ||
|
Protein |
nei |
Experiment(s):EBI-888807, EBI-882379 | ||
|
Protein |
ihfA |
Experiment(s):EBI-893916 | ||
|
Protein |
ihfB |
Experiment(s):EBI-893916 | ||
|
Protein |
hns |
Experiment(s):EBI-893916 | ||
|
Protein |
hupA |
Experiment(s):EBI-893916 | ||
|
Protein |
hupB |
Experiment(s):EBI-893916 | ||
|
Protein |
lpdA |
Experiment(s):EBI-893916 | ||
|
Protein |
malP |
Experiment(s):EBI-893916 | ||
|
Protein |
groL |
Experiment(s):EBI-893916 | ||
|
Protein |
rplC |
Experiment(s):EBI-893916 | ||
|
Protein |
rplD |
Experiment(s):EBI-893916 | ||
|
Protein |
rplL |
Experiment(s):EBI-893916 | ||
|
Protein |
rplS |
Experiment(s):EBI-893916 | ||
|
Protein |
rpmB |
Experiment(s):EBI-893916 | ||
|
Protein |
rpoA |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsE |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsF |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsG |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsM |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsN |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsO |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsR |
Experiment(s):EBI-893916 | ||
|
Protein |
ruvA |
Experiment(s):EBI-893916 | ||
|
Protein |
rdgC |
Experiment(s):EBI-893916 | ||
|
Protein |
rpsR |
LCMS(ID Probability):99.4 | ||
|
Protein |
groS |
LCMS(ID Probability):99.6 | ||
|
Protein |
groL |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsN |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsF |
LCMS(ID Probability):99.6 | ||
|
Protein |
hns |
LCMS(ID Probability):99.6 | ||
|
Protein |
ihfA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplS |
LCMS(ID Probability):99.6 | ||
|
Protein |
ruvA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsM |
LCMS(ID Probability):99.6 | ||
|
Protein |
malP |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplD |
LCMS(ID Probability):99.6 | ||
|
Protein |
hupA |
LCMS(ID Probability):99.6 | ||
|
Protein |
ihfB |
LCMS(ID Probability):99.6 | ||
|
Protein |
rdgC |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpoA |
LCMS(ID Probability):99.6 | ||
|
Protein |
hupB |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplL |
LCMS(ID Probability):99.6 | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MIMDLASLRA QQIELASSVI REDRLDKDPP DLIAGADVGF EQGGEVTRAA MVLLKYPSLE LVEYKVARIA TTMPYIPGFL SFREYPALLA AWEMLSQKPD LVFVDGHGIS HPRRLGVASH FGLLVDVPTI GVAKKRLCGK FEPLSSEPGA LAPLMDKGEQ LAWVWRSKAR CNPLFIATGH RVSVDSALAW VQRCMKGYRL PEPTRWADAV ASERPAFVRY TANQP |
| Length |
225 |
| Mol. Wt |
24.916 kDa |
| pI |
8.4 (calculated) |
| Extinction coefficient |
36,440 - 36,815 (calc based on 6 Y, 5 W, and 3 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
ASAP:ABE-0013068 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EcoCyc:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB:ECK120001843 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB1859 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
Ecoli K-12 |
5.986+/-0.056 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
|
mRNA |
Ecoli K-12 |
0.084947839 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
|
Protein |
E. coli K-12 MG1655 |
169 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
229 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
204 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
| edit table |
Notes
Transcription and Transcriptional Regulation
|
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<gbrowseImage> name=NC_000913:4196793..4196833 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for nfi | |
|
microarray |
Summary of data for nfi from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
| edit table |
<protect>
Notes
Accessions Related to nfi Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB1859 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120001843 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0013068 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
| Organism | Homologs (Statistics) | Comments |
|---|---|---|
|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
|
Bos taurus |
|
From Inparanoid:20070104 |
|
Canis familiaris |
|
From Inparanoid:20070104 |
|
Ciona intestinalis |
|
From Inparanoid:20070104 |
|
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
|
Gallus gallus |
|
From Inparanoid:20070104 |
|
Homo sapiens |
|
From Inparanoid:20070104 |
|
Macaca mulatta |
|
From Inparanoid:20070104 |
|
Monodelphis domestica |
|
From Inparanoid:20070104 |
|
Mus musculus |
|
From Inparanoid:20070104 |
|
Oryza gramene |
|
From Inparanoid:20070104 |
|
Pan troglodytes |
|
From Inparanoid:20070104 |
|
Rattus norvegicus |
|
From Inparanoid:20070104 |
|
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
|
Takifugu rubripes |
|
From Inparanoid:20070104 |
|
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
|
Xenopus tropicalis |
|
From Inparanoid:20070104 |
|
Shigella flexneri |
NFI |
From SHIGELLACYC |
|
E. coli O157 |
NFI |
From ECOO157CYC |
| edit table |
Do-It-Yourself Web Tools
Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11915 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120001843 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB1859 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0013068 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 7.23 7.24 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 8.16 8.17 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000287 ! magnesium ion binding
- GO:0004518 ! nuclease activity
- GO:0004519 ! endonuclease activity
- GO:0005737 ! cytoplasm
- GO:0006281 ! DNA repair
- GO:0006974 ! cellular response to DNA damage stimulus
- GO:0016787 ! hydrolase activity
- GO:0016788 ! hydrolase activity, acting on ester bonds
- GO:0043737 ! deoxyribonuclease V activity
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Dictyostelium discoideum
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Oryza gramene
- Genes with homologs in Pan troglodytes
- Genes with homologs in Rattus norvegicus
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157



