nfi:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Nfi

Synonyms

endonuclease V[1], B3998[2][1], YjaF[2][1], YfaF[2][1], Nfi[2][1], EndoV[2][1] , ECK3990, JW5547, yjaF, b3998

Product description

endonuclease V (deoxyinosine 3'endoduclease)[2][3]

Endonuclease V, specific for single-stranded DNA or duplex DNA with damaged U[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00801

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007581

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00801

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00801

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007581

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0016788

hydrolase activity, acting on ester bonds

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00801

F

Seeded from EcoCyc (v14.0)

complete

GO:0043737

deoxyribonuclease V activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.21.7

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceF

PMID:15690043[5]

Experiment(s):EBI-888807, EBI-893916

Protein

nei

PMID:15690043[5]

Experiment(s):EBI-888807, EBI-882379

Protein

ihfA

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

ihfB

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

hns

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

hupA

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

hupB

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

lpdA

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

malP

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

groL

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rplC

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rplD

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rplL

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rplS

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpmB

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpoA

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsE

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsF

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsG

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsM

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsN

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsO

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsR

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

ruvA

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rdgC

PMID:15690043[5]

Experiment(s):EBI-893916

Protein

rpsR

PMID:19402753[6]

LCMS(ID Probability):99.4

Protein

groS

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

groL

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsF

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

hns

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

ihfA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

ruvA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpsM

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

malP

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

ihfB

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rdgC

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpoA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

hupB

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753[6]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIMDLASLRA QQIELASSVI REDRLDKDPP DLIAGADVGF EQGGEVTRAA MVLLKYPSLE
LVEYKVARIA TTMPYIPGFL SFREYPALLA AWEMLSQKPD LVFVDGHGIS HPRRLGVASH
FGLLVDVPTI GVAKKRLCGK FEPLSSEPGA LAPLMDKGEQ LAWVWRSKAR CNPLFIATGH
RVSVDSALAW VQRCMKGYRL PEPTRWADAV ASERPAFVRY TANQP
Length

225

Mol. Wt

24.916 kDa

pI

8.4 (calculated)

Extinction coefficient

36,440 - 36,815 (calc based on 6 Y, 5 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

9..209

PF04493 Endonuclease V

PMID:19920124[7]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nfi taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111673

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948502

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013068

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P68739

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11915

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11915

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948502

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001843

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1859

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 5.18 5.19 5.20 5.21 5.22 5.23 5.24 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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