narG:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

narG

Gene Synonym(s)

ECK1218, b1224, JW1215, chlC, narC, bisD[1][2]

Product Desc.

nitrate reductase A, α subunit[2][3];

Component of NarGH catalytic dimer of nitrate reductase A[3]; nitrate reductase A[2][3]

Nitrate reductase I (NRA), alpha subunit[4]

Product Synonyms(s)

nitrate reductase 1, alpha subunit[1], B1224[2][1], ChlC[2][1], NarC[2][1], BisD[2][1], NarG[2][1] , chlC, ECK1218, JW1215, narC, b1224

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): narGHJI[2], OP00060, chlC, narG, narGHIJ

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Induced by anaerobiosis plus nitrate. NarG forms interactions with Mo proteins (MogA, MoeA, MobA and MobB), in the presence of NarJ, following the completion of Moco biosynthesis. HT_Cmplx14_Mem: NarG+NarH. NarG is anchored to the cytoplasmic side of the inner membrane. NarJ is essential for a Tat-dependent interaction between NarG and Mo proteins.[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

narG

Mnemonic

Nitrate reductase, nitrate regulation

Synonyms

ECK1218, b1224, JW1215, chlC, narC, bisD[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.57 minutes 

MG1655: 1279087..1282830
<gbrowseImage> name=NC_000913:1279087..1282830 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1280014..1283757
<gbrowseImage> name=NC_012967:1280014..1283757 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1167808..1171551
<gbrowseImage> name=NC_012759:1167808..1171551 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1281441..1285184
<gbrowseImage> name=NC_007779:1281441..1285184 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1319328..1323071
<gbrowseImage> name=NC_010473:1319328..1323071 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1279090

Edman degradation

PMID:3053688


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

narG(del) (Keio:JW1215)

deletion

deletion

PMID:16738554

Shigen

narG::Tn5KAN-2 (FB20270)

Insertion at nt 966 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20270

does not contain pKD46

narGH50S

H50S

Loss of activity

seeded from UniProt:P09152

narG -

Enzyme Activity

reduced Nutrate recutase activity (roughly 1% of wildtype activity).

PMID:7047497

Experimental strain: RK5265

table 5

narG -

Enzyme Activity

Reduced Formate activity

PMID:7047497

Experimental strain: RK5265

table 5

narC

Resistant to

Resistant to Chlorate

PMID:4558444

Strain: PK 27

Table 7

narC

Enzyme Activity

lack of formate nitrate reductase activity

PMID:4558444

Strain: PK 27

Table 7

narC

Resistant to

Ressitant to Chlorate

PMID:4904136

Strains: W, WGA and WGAC derivatives


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1215

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTAAATTCCTGGACCGGTT

Primer 2:CCTTTTACGCTCTCCTGTACCTG

13H6

Kohara Phage

Genobase

PMID:3038334

12H9

Kohara Phage

Genobase

PMID:3038334

4D8

Kohara Phage

Genobase

PMID:3038334

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[5]

est. P1 cotransduction: 14% [6]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[5]

est. P1 cotransduction: 40% [6]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10638

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10638

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000631

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945782

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0632

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004119

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NarG

Synonyms

nitrate reductase 1, alpha subunit[1], B1224[2][1], ChlC[2][1], NarC[2][1], BisD[2][1], NarG[2][1] , chlC, ECK1218, JW1215, narC, b1224

Product description

nitrate reductase A, α subunit[2][3];

Component of NarGH catalytic dimer of nitrate reductase A[3]; nitrate reductase A[2][3]

Nitrate reductase I (NRA), alpha subunit[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006468

F

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.7.99.4

F

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

PMID:11844760

IGI: Inferred from Genetic Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0008940

nitrate reductase activity

PMID:11844760

IGI: Inferred from Genetic Interaction

EcoliWiki:napA

F

Seeded from EcoCyc (v14.0)

complete

GO:0009055

electron carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006655

F

Seeded from EcoCyc (v14.0)

complete

GO:0009055

electron carrier activity

PMID:10504245

IPI: Inferred from Physical Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0009061

anaerobic respiration

PMID:7050087

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

GO:0009325

nitrate reductase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006468

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0017004

cytochrome complex assembly

PMID:3053688

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

GO:0022900

electron transport chain

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0249

P

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006657

F

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0500

F

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

PMID:14725769

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0031224

intrinsic to membrane

PMID:5996

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0042126

nitrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006468

P

Seeded from EcoCyc (v14.0)

complete

GO:0042128

nitrate assimilation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0534

P

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

PMID:14725769

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006468

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of NarGH catalytic dimer of nitrate reductase A

could be indirect

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1139222

Protein

recA

PMID:15690043

Experiment(s):EBI-880367

Protein

hldD

PMID:15690043

Experiment(s):EBI-880367

Protein

tufA

PMID:15690043

Experiment(s):EBI-880367

Protein

rfaD

PMID:19402753

MALDI(Z-score):32.746440

Protein

dnaJ

PMID:19402753

MALDI(Z-score):37.020045

Protein

mreB

PMID:19402753

MALDI(Z-score):30.550995

Protein

Subunits of nitrate reductase A

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSKFLDRFRY FKQKGETFAD GHGQLLNTNR DWEDGYRQRW QHDKIVRSTH GVNCTGSCSW
KIYVKNGLVT WETQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPMMRKRLMK
MWREAKALHS DPVEAWASII EDADKAKSFK QARGRGGFVR SSWQEVNELI AASNVYTIKN
YGPDRVAGFS PIPAMSMVSY ASGARYLSLI GGTCLSFYDW YCDLPPASPQ TWGEQTDVPE
SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTVA VTPDYAEIAK LCDLWLAPKQ
GTDAAMALAM GHVMLREFHL DNPSQYFTDY VRRYTDMPML VMLEERDGYY AAGRMLRAAD
LVDALGQENN PEWKTVAFNT NGEMVAPNGS IGFRWGEKGK WNLEQRDGKT GEETELQLSL
LGSQDEIAEV GFPYFGGDGT EHFNKVELEN VLLHKLPVKR LQLADGSTAL VTTVYDLTLA
NYGLERGLND VNCATSYDDV KAYTPAWAEQ ITGVSRSQII RIAREFADNA DKTHGRSMII
VGAGLNHWYH LDMNYRGLIN MLIFCGCVGQ SGGGWAHYVG QEKLRPQTGW QPLAFALDWQ
RPARHMNSTS YFYNHSSQWR YETVTAEELL SPMADKSRYT GHLIDFNVRA ERMGWLPSAP
QLGTNPLTIA GEAEKAGMNP VDYTVKSLKE GSIRFAAEQP ENGKNHPRNL FIWRSNLLGS
SGKGHEFMLK YLLGTEHGIQ GKDLGQQGGV KPEEVDWQDN GLEGKLDLVV TLDFRLSSTC
LYSDIILPTA TWYEKDDMNT SDMHPFIHPL SAAVDPAWEA KSDWEIYKAI AKKFSEVCVG
HLGKETDIVT LPIQHDSAAE LAQPLDVKDW KKGECDLIPG KTAPHIMVVE RDYPATYERF
TSIGPLMEKI GNGGKGIAWN TQSEMDLLRK LNYTKAEGPA KGQPMLNTAI DAAEMILTLA
PETNGQVAVK AWAALSEFTG RDHTHLALNK EDEKIRFRDI QAQPRKIISS PTWSGLEDEH
VSYNAGYTNV HELIPWRTLS GRQQLYQDHQ WMRDFGESLL VYRPPIDTRS VKEVIGQKSN
GNQEKALNFL TPHQKWGIHS TYSDNLLMLT LGRGGPVVWL SEADAKDLGI ADNDWIEVFN
SNGALTARAV VSQRVPAGMT MMYHAQERIV NLPGSEITQQ RGGIHNSVTR ITPKPTHMIG
GYAHLAYGFN YYGTVGSNRD EFVVVRKMKN IDWLDGEGND QVQESVK
Length

1,247

Mol. Wt

140.491 kDa

pI

6.5 (calculated)

Extinction coefficient

287,970 - 289,470 (calc based on 53 Y, 38 W, and 12 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P09152

Domain

108..833

PF00384 Molybdopterin oxidoreductase

PMID:19920124

Domain

1087..1206

PF01568 Molydopterin dinucleotide binding domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=narG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129187

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945782

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004119

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P09152

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10638

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10638

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945782

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000631

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0632

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.01E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

2.978+/-0.036

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.001736575

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

3

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

14

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

narGHJI

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1279067..1279107 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1224 (EcoliWiki Page)

NCBI GEO profiles for narG

microarray

GenExpDB:b1224 (EcoliWiki Page)

Summary of data for narG from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to narG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10638

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0632

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1224

EcoGene

EcoGene:EG10638

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000631

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004119

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000033266 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

NARG

From SHIGELLACYC

E. coli O157

NARG

From ECOO157CYC

pyrobaculum aerophilum

NARG

[7]


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00384 Molybdopterin oxidoreductase

Pfam (EcoliWiki Page)

PF01568 Molydopterin dinucleotide binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:50692

Superfamily (EcoliWiki Page)

SUPERFAMILY:53706

EcoCyc

EcoCyc:EG10638

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10638

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000631

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0632

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004119

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Fitz-Gibbon, ST et al. (2002) Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. Proc. Natl. Acad. Sci. U.S.A. 99 984-9 PubMed

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