narG:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
narG |
|---|---|
| Mnemonic |
Nitrate reductase, nitrate regulation |
| Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
27.57 minutes |
MG1655: 1279087..1282830 |
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NC_012967: 1280014..1283757 |
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NC_012759: 1167808..1171551 |
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W3110 |
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W3110: 1281441..1285184 |
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DH10B: 1319328..1323071 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
1279090 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
narG(del) (Keio:JW1215) |
deletion |
deletion |
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narG::Tn5KAN-2 (FB20270) |
Insertion at nt 966 in Plus orientation |
does not contain pKD46 | |||||
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narGH50S |
H50S |
Loss of activity |
seeded from UniProt:P09152 | ||||
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narG - |
Enzyme Activity |
reduced Nutrate recutase activity (roughly 1% of wildtype activity). |
Experimental strain: RK5265 |
table 5 | |||
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narG - |
Enzyme Activity |
Reduced Formate activity |
Experimental strain: RK5265 |
table 5 | |||
|
narC |
Resistant to |
Resistant to Chlorate |
Strain: PK 27 |
Table 7 | |||
|
narC |
Enzyme Activity |
lack of formate nitrate reductase activity |
Strain: PK 27 |
Table 7 | |||
|
narC |
Resistant to |
Ressitant to Chlorate |
Strains: W, WGA and WGAC derivatives |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1215 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGTAAATTCCTGGACCGGTT Primer 2:CCTTTTACGCTCTCCTGTACCTG | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 14% [12] | ||
|
Linked marker |
est. P1 cotransduction: 40% [12] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Sodergren, EJ et al. (1988) Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli. J. Biol. Chem. 263 16156-62 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 Stewart, V & MacGregor, CH (1982) Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci. J. Bacteriol. 151 788-99 PubMed
- ↑ 7.0 7.1 Glaser, JH & DeMoss, JA (1972) Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol. Gen. Genet. 116 1-10 PubMed
- ↑ Guest, JR (1969) Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli. Mol. Gen. Genet. 105 285-97 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 10.0 10.1 10.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 11.0 11.1 CGSC: The Coli Genetics Stock Center
- ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
