mhpC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

mhpC

Gene Synonym(s)

ECK0346, b0349, JW0340[1], JW0340

Product Desc.
Product Synonyms(s)

2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase[1], B0349[2][1], MhpC[2][1] , ECK0346, JW0340, b0349

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mhpABCDFE[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mhpC

Mnemonic

m-Hydroxyphenylpropionic acid

Synonyms

ECK0346, b0349, JW0340[1], JW0340

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

7.98 minutes 

MG1655: 370448..371329
<gbrowseImage> name=NC_000913:370448..371329 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 343780..344661
<gbrowseImage> name=NC_012967:343780..344661 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 370448..371329
<gbrowseImage> name=NC_007779:370448..371329 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1389891..1390772
<gbrowseImage> name=NC_010473:1389891..1390772 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mhpC(del) (Keio:JW0340)

deletion

deletion

PMID:16738554

Shigen

CGSC8531[3]

mhpCR197K

R197K

40-fold and 5-fold decrease in catalytic activity and affinity, respectively

seeded from UniProt:P77044

mhpCR197Q

R197Q

280-fold and 10-fold decrease in catalytic activity and affinity, respectively

seeded from UniProt:P77044

mhpCS119A

S119A

Weakly active. 3-fold decrease in affinity. Fast ketonisation and slow C-C cleavage

seeded from UniProt:P77044

mhpCC270A

C270A

2-fold decrease in catalytic activity and almost 2-fold increase in affinity

seeded from UniProt:P77044

mhpCH272A

H272A

Weakly active, 1000-fold decrease in catalytic efficiency. Very slow ketonisation and C-C cleavage

seeded from UniProt:P77044

mhpCW273G

W273G

10-fold and 20-fold decrease in catalytic activity and affinity, respectively

seeded from UniProt:P77044

mhpCS119G

S119G

Weakly active. 3-fold decrease in affinity. Fast ketonisation and slow C-C cleavage

seeded from UniProt:P77044

mhpCH123A

H123A

More than 2-fold decrease in catalytic efficiency and 3-fold increase affinity

seeded from UniProt:P77044

mhpCS49A

S49A

2-fold decrease in catalytic efficiency and more than 5-fold increase in affinity for the natural substrate

seeded from UniProt:P77044

mhpCN118A

N118A

200-fold decrease in catalytic activity and almost 2-fold increase in affinity

seeded from UniProt:P77044

mhpCN118H

N118H

350-fold decrease in catalytic activity and almost 2-fold increase in affinity

seeded from UniProt:P77044

mhpCF182D

F182D

100-fold decrease in catalytic activity

seeded from UniProt:P77044

mhpCF182G

F182G

4-fold and 8-fold decrease in catalytic activity and affinity, respectively

seeded from UniProt:P77044

mhpC281::Tn10

Insertion at 371,180 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12080 = CGSC7333[3]

Synonyms: zah-281::Tn10

mhpC281::Tn10

PMID:2540407 PMID:9829956

CGSC:12295

ΔmhpC789::kan

PMID:16738554

CGSC:101860

MhpC H263A

H263A

Ser110, His263 and Asp235 compose the catalytic triad of MhpC, and mutation of His263 to Alanine causes a 10,000 fold reduction in Kcat for 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity.

PMID:15663941

MhpC Ser110A MhpC Ser110G

Either: Ser110Ala or Ser110Gly

Ser110, His263 and Asp235 compose the catalytic triad of MhpC, and mutation of Ser110 to Alanine or Glycine causes a 10,000 fold reduction in Kcat for 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity.

PMID:15663941


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0340

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCAGGAGAAGATGATGAGTTA

Primer 2:CCAGGGCGTGCGAGGAAATTCAG

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[3]

est. P1 cotransduction: 88% [4]
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[3]

est. P1 cotransduction: 41% [4]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:M012

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20275

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004471

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944954

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4168

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001201

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MhpC

Synonyms

2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase[1], B0349[2][1], MhpC[2][1] , ECK0346, JW0340, b0349

Product description
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0005737

cytoplasm

PMID:9315862

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0042803

protein homodimerization activity

PMID:9315862

IPI: Inferred from Physical Interaction

F

Missing: with/from

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

PMID:9315862

IDA: Inferred from Direct Assay

F

MhpC was shown to have 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity.

complete

GO:0016823

hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.7.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

PMID:3531186

IMP: Inferred from Mutant Phenotype

P

Cells containing mutations in mhpC grow as yellow colonies on CEM (color enhancing media) plates containing either 3-(3-hydroxyphenyl)propionic acid (m-hydroxyphenylpropionic acid, MHP) or 3-phenylpropionic acid (hydrocinnamic acid, HCA).

complete

GO:0018771

2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01654

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

PMID:15663941

IDA: Inferred from Direct Assay

F

MhpC was shown to have 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity.

complete

GO:0019439

aromatic compound catabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01654

P

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0058

P

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:9315862

IDA: Inferred from Direct Assay

C

The preparation of MhpC performed as described in PMID:8399388

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

norV

PMID:16606699

Experiment(s):EBI-1136328

Protein

cydC

PMID:16606699

Experiment(s):EBI-1136328

Protein

groL

PMID:16606699

Experiment(s):EBI-1136328
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MDCRIWLVKR QNRELNMQEK MMSYQPQTEA ATSRFLNVEE AGKTLRIHFN DCGQGDETVV
LLHGSGPGAT GWANFSRNID PLVEAGYRVI LLDCPGWGKS DSVVNSGSRS DLNARILKSV
VDQLDIAKIH LLGNSMGGHS SVAFTLKWPE RVGKLVLMGG GTGGMSLFTP MPTEGIKRLN
QLYRQPTIEN LKLMMDIFVF DTSDLTDALF EARLNNMLSR RDHLENFVKS LEANPKQFPD
FGPRLAEIKA QTLIVWGRND RFVPMDAGLR LLSGIAGSEL HIFRDCGHWA QWEHADAFNQ
LVLNFLARP
Length

309

Mol. Wt

34.642 kDa

pI

7.1 (calculated)

Extinction coefficient

42,970 - 43,470 (calc based on 3 Y, 7 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

71..288

PF00561 alpha/beta hydrolase fold

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mhpC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111115

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944954

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001201

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77044

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:M012

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20275

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944954

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004471

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4168

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

15

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

26

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mhpABCDFE

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:370428..370468 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0349 (EcoliWiki Page)

NCBI GEO profiles for mhpC

microarray

GenExpDB:b0349 (EcoliWiki Page)

Summary of data for mhpC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to mhpC Expression

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Database Accession Notes

EcoCyc

EcoCyc:M012

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4168

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0349

EcoGene

EcoGene:EG20275

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004471

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001201

Escherichia coli str. K-12 substr. MG1655

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000013613 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT1G72620 (score: 1.000; bootstrap: 100%)
  • AT1G17430 (score: 0.748)
  • AT5G09430 (score: 0.173)
  • AT4G33180 (score: 0.163)
  • AT1G78210 (score: 0.156)
  • AT5G21950 (score: 0.153)
  • AT4G39955 (score: 0.141)
  • AT2G18360 (score: 0.121)
  • AT4G36610 (score: 0.111)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000022100 (score: 1.000; bootstrap: 53%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00016507 (score: 1.000; bootstrap: 53%)
  • WBGene00016506 (score: 0.623)
  • WBGene00007711 (score: 0.176)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000010991 (score: 1.000; bootstrap: 52%)
  • ENSCAFP00000029897 (score: 1.000; bootstrap: 53%)
  • ENSCAFP00000023703 (score: 0.344)
  • ENSCAFP00000012415 (score: 0.171)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000013631 (score: 1.000; bootstrap: 100%)
  • ENSCINP00000010598 (score: 0.926)
  • ENSCINP00000013626 (score: 0.218)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0184562 (score: 1.000; bootstrap: 63%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA18833-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000009676 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000026689 (score: 0.097)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000305683 (score: 1.000; bootstrap: 53%)
  • ENSP00000221730 (score: 0.377)
  • ENSP00000315621 (score: 0.139)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000008314 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000014869 (score: 1.000; bootstrap: 56%)
  • ENSMODP00000018168 (score: 0.366)

From Inparanoid:20070104

Mus musculus

  • MGI:99500 (score: 1.000; bootstrap: 62%)
  • MGI:1919182 (score: 0.066)
  • MGI:2686228 (score: 0.065)

From Inparanoid:20070104

Oryza gramene

  • Q7XI46 (score: 1.000; bootstrap: 63%)
  • Q75LP9 (score: 0.669)
  • Q8RUY8 (score: 0.508)
  • Q7G6Q1 (score: 0.508)
  • Q8H024 (score: 0.193)
  • Q8H026 (score: 0.171)
  • Q9AS70 (score: 0.164)
  • Q8H5Y0 (score: 0.156)
  • Q8S3R0 (score: 0.141)
  • Q8H025 (score: 0.136)
  • Q5VNP5 (score: 0.129)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000001645 (score: 1.000; bootstrap: 59%)
  • ENSPTRP00000018121 (score: 0.337)
  • ENSPTRP00000034421 (score: 0.136)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000023914 (score: 1.000; bootstrap: 52%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YNR064C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC6G103c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000147298 (score: 1.000; bootstrap: 56%)
  • NEWSINFRUP00000139867 (score: 0.217)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00027513001 (score: 1.000; bootstrap: 56%)
  • GSTENP00027407001 (score: 0.100)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000014148 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104

E. coli O157

MHPC

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00561 alpha/beta hydrolase fold

Superfamily (EcoliWiki Page)

SUPERFAMILY:53474

EcoCyc

EcoCyc:M012

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20275

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004471

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4168

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001201

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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