mhpC:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MhpC

Synonyms

2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase[1], B0349[2][1], MhpC[2][1] , ECK0346, JW0340, b0349

Product description
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0005737

cytoplasm

PMID:9315862[3]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0042803

protein homodimerization activity

PMID:9315862[3]

IPI: Inferred from Physical Interaction

F

Missing: with/from

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

PMID:9315862[3]

IDA: Inferred from Direct Assay

F

MhpC was shown to have 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity.

complete

GO:0016823

hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.7.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

PMID:3531186[4]

IMP: Inferred from Mutant Phenotype

P

Cells containing mutations in mhpC grow as yellow colonies on CEM (color enhancing media) plates containing either 3-(3-hydroxyphenyl)propionic acid (m-hydroxyphenylpropionic acid, MHP) or 3-phenylpropionic acid (hydrocinnamic acid, HCA).

complete

GO:0018771

2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01654

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

PMID:15663941[5]

IDA: Inferred from Direct Assay

F

MhpC was shown to have 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity.

complete

GO:0019439

aromatic compound catabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01654

P

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0058

P

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:9315862[3]

IDA: Inferred from Direct Assay

C

The preparation of MhpC performed as described in PMID:8399388[6]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

norV

PMID:16606699[7]

Experiment(s):EBI-1136328

Protein

cydC

PMID:16606699[7]

Experiment(s):EBI-1136328

Protein

groL

PMID:16606699[7]

Experiment(s):EBI-1136328
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MDCRIWLVKR QNRELNMQEK MMSYQPQTEA ATSRFLNVEE AGKTLRIHFN DCGQGDETVV
LLHGSGPGAT GWANFSRNID PLVEAGYRVI LLDCPGWGKS DSVVNSGSRS DLNARILKSV
VDQLDIAKIH LLGNSMGGHS SVAFTLKWPE RVGKLVLMGG GTGGMSLFTP MPTEGIKRLN
QLYRQPTIEN LKLMMDIFVF DTSDLTDALF EARLNNMLSR RDHLENFVKS LEANPKQFPD
FGPRLAEIKA QTLIVWGRND RFVPMDAGLR LLSGIAGSEL HIFRDCGHWA QWEHADAFNQ
LVLNFLARP
Length

309

Mol. Wt

34.642 kDa

pI

7.1 (calculated)

Extinction coefficient

42,970 - 43,470 (calc based on 3 Y, 7 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

71..288

PF00561 alpha/beta hydrolase fold

PMID:19920124[8]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mhpC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111115

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944954

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001201

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77044

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:M012

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20275

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944954

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004471

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4168

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 Lam, WW & Bugg, TD (1997) Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase. Biochemistry 36 12242-51 PubMed
  4. ↑ Burlingame, RP et al. (1986) Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation. J. Bacteriol. 168 55-64 PubMed
  5. ↑ Li, C et al. (2005) Catalytic mechanism of C-C hydrolase MhpC from Escherichia coli: kinetic analysis of His263 and Ser110 site-directed mutants. J. Mol. Biol. 346 241-51 PubMed
  6. ↑ Bugg, TD (1993) Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli. Biochim. Biophys. Acta 1202 258-64 PubMed
  7. ↑ 7.0 7.1 7.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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