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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	lamB
Gene Synonym(s)	ECK4028, b4036, JW3996, malB, malL^[1], malL
Product Desc.	phage lambda receptor protein; maltose high-affinity receptor^[2]^[3]; Component of Maltose/Maltodextrin Transport System^[3] Maltoporin, maltose high-affinity uptake system; phage lambda receptor protein^[4]
Product Synonyms(s)	maltose outer membrane porin (maltoporin)^[1], B4036^[2]^[1], MalL^[2]^[1], MalB^[2]^[1], LamB^[2]^[1] , ECK4028, JW3996, malB, malL, b4036
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): malK-lamB-malM^[2], OP00165
Regulation/Activity
Quick Links
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Notes

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LamB also facilitates the diffusion of maltooligosaccharides, i.e maltodextrin. MalT regulon. Binds TrxA (Kumar, 2004).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lamB
Mnemonic	Lambda
Synonyms	ECK4028, b4036, JW3996, malB, malL^[1], malL
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	91.51 minutes	MG1655: 4245994..4247334 <gbrowseImage> name=NC_000913:4245994..4247334 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4226903..4228243 <gbrowseImage> name=NC_012967:4226903..4228243 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4184839..4186179 <gbrowseImage> name=NC_012759:4184839..4186179 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4251561..4252901 <gbrowseImage> name=NC_007779:4251561..4252901 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4345690..4347030 <gbrowseImage> name=NC_010473:4345690..4347030 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4246069	Edman degradation	PMID:346375
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔlamB (Keio:JW3996)	deletion	deletion			PMID:16738554	Shigen CGSC10877^[5]
lamBR134D,N		R134D,N		Slight increase in pore size; increase in sucrose transport and slight decrease in maltose transport; when associated with D-143 and F-146			seeded from UniProt:P02943
lamBY143F		Y143F		Increases affinity for starch and maltose, pore size and transport of maltohexaose			seeded from UniProt:P02943
lamBD146G		D146G		Increases affinity for large dextrins			seeded from UniProt:P02943
lamBW99R		W99R		Decreases starch affinity, slightly increases maltose affinity			seeded from UniProt:P02943
lamBR107S		R107S		Decreases maltodextrin affinity, increases sucrose binding and transport			seeded from UniProt:P02943
lamBR134A		R134A		Considerable increase in pore size and sucrose transport, slight decrease in maltose transport; when associated with A-143			seeded from UniProt:P02943
lamBR33H		R33H		Reduces ligand affinity and pore size, no longer selective for maltodextrins			seeded from UniProt:P02943
ΔlamB::kan	deletion		Biolog:respiration	unable to respire Dextrin	PMID:16095938
lamB63					PMID:778586 PMID:329879	CGSC:8261
lamB204(Am)						CGSC:9968	amber (UAG) mutation
lamB60::Tn10						CGSC:12195
lamB20::Tn5						CGSC:14652
lamB2000(LamR)				lambda resistant		CGSC:65959
ΔlamB732::kan					PMID:16738554	CGSC:101636
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3996	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGATTACTCTGCGCAAACT Primer 2:CCCCACCAGATTTCCATCTGGGC
1F8	Kohara Phage	Genobase	PMID:3038334
12B4	Kohara Phage	Genobase	PMID:3038334
malF3089::Tn10	Linked marker	CAG12164 = CGSC7476^[5]	est. P1 cotransduction: 84% ^[6]
zje-2241::Tn10	Linked marker	CAG18427 = CGSC7481^[5]	est. P1 cotransduction: 5% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10528	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10528	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000521	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948548	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0523	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013218	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LamB
Synonyms	maltose outer membrane porin (maltoporin)^[1], B4036^[2]^[1], MalL^[2]^[1], MalB^[2]^[1], LamB^[2]^[1] , ECK4028, JW3996, malB, malL, b4036
Product description	phage lambda receptor protein; maltose high-affinity receptor^[2]^[3]; Component of Maltose/Maltodextrin Transport System^[3] Maltoporin, maltose high-affinity uptake system; phage lambda receptor protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005351	sugar:hydrogen symporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003192	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003192	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006811	ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008643	carbohydrate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01301	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009597	detection of virus	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0580	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015288	porin activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01301	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015768	maltose transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01301	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003192	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046718	entry of virus into host cell	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0580	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046930	pore complex	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0626	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Maltose/Maltodextrin Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Outer Membrane	PMID:346375	EchoLocation:lamB
outer membrane		From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMITLRKLPL AVAVAAGVMS AQAMAVDFHG YARSGIGWTG SGGEQQCFQT TGAQSKYRLG NECETYAELK LGQEVWKEGD KSFYFDTNVA YSVAQQNDWE ATDPAFREAN VQGKNLIEWL PGSTIWAGKR FYQRHDVHMI DFYYWDISGP GAGLENIDVG FGKLSLAATR SSEAGGSSSF ASNNIYDYTN ETANDVFDVR LAQMEINPGG TLELGVDYGR ANLRDNYRLV DGASKDGWLF TAEHTQSVLK GFNKFVVQYA TDSMTSQGKG LSQGSGVAFD NEKFAYNINN NGHMLRILDH GAISMGDNWD MMYVGMYQDI NWDNDNGTKW WTVGIRPMYK WTPIMSTVME IGYDNVESQR TGDKNNQYKI TLAQQWQAGD SIWSRPAIRV FATYAKWDEK WGYDYTGNAD NNANFGKAVP ADFNGGSFGR GDSDEWTFGA QMEIWW
Length	446
Mol. Wt	49.912 kDa
pI	4.7 (calculated)
Extinction coefficient	137,280 - 137,530 (calc based on 22 Y, 19 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-25	UniProt Manual:Signal Peptides	UniProt:P02943
Domain	28..446	PF02264 LamB porin		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lamB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131862	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948548	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013218	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P02943	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10528	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10528	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948548	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000521	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0523	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.02E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 MG1655	100	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	314	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	13^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
malK-lamB-malM
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

There is a pleiotropic decrease in expression of lamB with the addition of procaine. ^[7]

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4245974..4246014 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4036 (EcoliWiki Page)	NCBI GEO profiles for lamB
microarray	GenExpDB:b4036 (EcoliWiki Page)	Summary of data for lamB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to lamB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10528	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0523	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4036
EcoGene	EcoGene:EG10528	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000521	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013218	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	LAMB	From SHIGELLACYC
E. coli O157	LAMB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02264 LamB porin
Superfamily (EcoliWiki Page)	SUPERFAMILY:56935
EcoCyc	EcoCyc:EG10528	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10528	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000521	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0523	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013218	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Garrett, S et al. (1983) Isolation and characterization of chain-terminating nonsense mutations in a porin regulator gene, envZ. J. Bacteriol. 156 62-9 PubMed

lamB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lamB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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