lamB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
lamB |
|---|---|
| Mnemonic |
Lambda |
| Synonyms |
ECK4028, b4036, JW3996, malB, malL[1], malL |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
91.51 minutes |
MG1655: 4245994..4247334 |
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NC_012967: 4226903..4228243 |
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NC_012759: 4184839..4186179 |
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W3110 |
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W3110: 4251561..4252901 |
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DH10B: 4345690..4347030 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4246069 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔlamB (Keio:JW3996) |
deletion |
deletion |
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lamBR134D,N |
R134D,N |
Slight increase in pore size; increase in sucrose transport and slight decrease in maltose transport; when associated with D-143 and F-146 |
seeded from UniProt:P02943 | ||||
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lamBY143F |
Y143F |
Increases affinity for starch and maltose, pore size and transport of maltohexaose |
seeded from UniProt:P02943 | ||||
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lamBD146G |
D146G |
Increases affinity for large dextrins |
seeded from UniProt:P02943 | ||||
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lamBW99R |
W99R |
Decreases starch affinity, slightly increases maltose affinity |
seeded from UniProt:P02943 | ||||
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lamBR107S |
R107S |
Decreases maltodextrin affinity, increases sucrose binding and transport |
seeded from UniProt:P02943 | ||||
|
lamBR134A |
R134A |
Considerable increase in pore size and sucrose transport, slight decrease in maltose transport; when associated with A-143 |
seeded from UniProt:P02943 | ||||
|
lamBR33H |
R33H |
Reduces ligand affinity and pore size, no longer selective for maltodextrins |
seeded from UniProt:P02943 | ||||
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ΔlamB::kan |
deletion |
Biolog:respiration |
unable to respire Dextrin |
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lamB63 |
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lamB204(Am) |
amber (UAG) mutation | ||||||
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lamB60::Tn10 |
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lamB20::Tn5 |
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lamB2000(LamR) |
lambda resistant |
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ΔlamB732::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3996 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATGATTACTCTGCGCAAACT Primer 2:CCCCACCAGATTTCCATCTGGGC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 84% [10] | ||
|
Linked marker |
est. P1 cotransduction: 5% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Edermann, R et al. (1978) Major proteins of the Escherichia coli outer cell envelope membrane. Preliminary characterization of the phage lambda receptor protein. FEBS Lett. 88 71-4 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Hofnung, M et al. (1976) lamB mutations in E. coli K12: growth of lambda host range mutants and effect of nonsense suppressors. Mol. Gen. Genet. 145 207-13 PubMed
- ↑ Braun, V & Krieger-Brauer, HJ (1977) Interrelationship of the phage lambda receptor protein and maltose transport in mutants of Escherichia coli K12. Biochim. Biophys. Acta 469 89-98 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
