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Expression|

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Standard Name	lacY
Gene Synonym(s)	ECK0340, b0343, JW0334^[1], JW0334
Product Desc.	LacY lactose MFS transporter^[2]^[3] Lactose permease; galactoside permease^[4]
Product Synonyms(s)	lactose/galactose transporter^[1], B0343^[2]^[1], lactose permease^[2]^[1], lac permease^[2]^[1], LacY^[2]^[1] , ECK0340, JW0334, b0343
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): lacZYA^[2], lacZ, OP00045, lacAYZ
Regulation/Activity
Quick Links
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Notes

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Major Facilitator Superfamily (MFS).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lacY
Mnemonic	Lactose
Synonyms	ECK0340, b0343, JW0334^[1], JW0334
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	7.78 minutes	MG1655: 362403..361150 <gbrowseImage> name=NC_000913:361150..362403 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 335735..334482 <gbrowseImage> name=NC_012967:334482..335735 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4174500..4175753 <gbrowseImage> name=NC_012759:4174500..4175753 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 362403..361150 <gbrowseImage> name=NC_007779:361150..362403 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1381939..1380686 <gbrowseImage> name=NC_010473:1380686..1381939 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
lacY(del) (Keio:JW0334)	deletion	deletion			PMID:16738554	Shigen
lacYD237N,G		D237N,G		Defect in melibiose transport			seeded from UniProt:P02920
lacYK358T		K358T		Defect in melibiose transport			seeded from UniProt:P02920
lacY1						CGSC:4495
lacY40						CGSC:5870
lacY29						CGSC:6818
lacY328(Am)						CGSC:7291	amber (UAG) mutation
lacY96						CGSC:7302
lacY0(Am)						CGSC:12982	amber (UAG) mutation
lacY41						CGSC:13218
lacY83						CGSC:14688
MAD59-lacY			Carbon Utilization	Unable to utiliza Malibose at 42C	PMID:330501	Experimental Strain: MAD59-lacY
MAD59-lacY			Carbon Utilization	Unable to utilize lactose at 42C	PMID:330501	Experimental Strain: MAD59-lacY
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0334	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:gccTACTATTTAAAAAACACAAACTTTTGG Primer 2:ccAGCGACTTCATTCACCTGACGACGCAGC
10A6	Kohara Phage	Genobase	PMID:3038334
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: % ^[6]
lacI3042::Tn10	Linked marker	CAG18439 = CGSC7334^[5]	est. P1 cotransduction: 84% ^[6] Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10526	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10526	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000519	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949083	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0521	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001181	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LacY
Synonyms	lactose/galactose transporter^[1], B0343^[2]^[1], lactose permease^[2]^[1], lac permease^[2]^[1], LacY^[2]^[1] , ECK0340, JW0334, b0343
Product description	LacY lactose MFS transporter^[2]^[3] Lactose permease; galactoside permease^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	Aspect	Notes	Status
	GO:0005887	integral to plasma membrane	PMID:7028742	IDA: Inferred from Direct Assay	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015528	lactose:hydrogen symporter activity	PMID:3520229	IDA: Inferred from Direct Assay	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015528	lactose:hydrogen symporter activity	PMID:7028742	IDA: Inferred from Direct Assay	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015767	lactose transport	PMID:3520229	IDA: Inferred from Direct Assay	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015767	lactose transport	PMID:7028742	IDA: Inferred from Direct Assay	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:4900505	IDA: Inferred from Direct Assay	C		complete
	GO:0030395	lactose binding	PMID:12893935	IDA: Inferred from Direct Assay	F		complete
	GO:0016021	integral to membrane	PMID:12893935	IDA: Inferred from Direct Assay	C		complete
	GO:0008643	carbohydrate transport	PMID:5541536	IMP: Inferred from Mutant Phenotype	P		complete
	GO:0005351	sugar:hydrogen symporter activity	PMID:7000781	IDA: Inferred from Direct Assay	F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 10 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MYYLKNTNFW MFGLFFFFYF FIMGAYFPFF PIWLHDINHI SKSDTGIIFA AISLFSLLFQ PLFGLLSDKL GLRKYLLWII TGMLVMFAPF FIFIFGPLLQ YNILVGSIVG GIYLGFCFNA GAPAVEAFIE KVSRRSNFEF GRARMFGCVG WALCASIVGI MFTINNQFVF WLGSGCALIL AVLLFFAKTD APSSATVANA VGANHSAFSL KLALELFRQP KLWFLSLYVI GVSCTYDVFD QQFANFFTSF FATGEQGTRV FGYVTTMGEL LNASIMFFAP LIINRIGGKN ALLLAGTIMS VRIIGSSFAT SALEVVILKT LHMFEVPFLL VGCFKYITSQ FEVRFSATIY LVCFCFFKQL AMIFMSVLAG NMYESIGFQG AYLVLGLVAL GFTLISVFTL SGPGPLSLLR RQVNEVA
Length	417
Mol. Wt	46.505 kDa
pI	8.9 (calculated)
Extinction coefficient	53,860 - 54,860 (calc based on 14 Y, 6 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..413	PF01306 LacY proton/sugar symporter		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lacY taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128328	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949083	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001181	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P02920	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10526	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10526	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949083	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000519	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0521	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	3^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	2^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
lacZYA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:362383..362423 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0343 (EcoliWiki Page)	NCBI GEO profiles for lacY
microarray	GenExpDB:b0343 (EcoliWiki Page)	Summary of data for lacY from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (362320..362518) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ15; Well:H10^[8]
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Notes

Accessions Related to lacY Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10526	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0521	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0343
EcoGene	EcoGene:EG10526	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000519	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001181	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000018634 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0051663 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	LACY	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01306 LacY proton/sugar symporter
Panther (EcoliWiki Page)	PTHR24003:SF271
Superfamily (EcoliWiki Page)	SUPERFAMILY:103473
EcoCyc	EcoCyc:EG10526	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10526	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000519	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0521	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001181	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

lacY:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lacY Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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