lacY:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LacY

Synonyms

lactose/galactose transporter[1], B0343[2][1], lactose permease[2][1], lac permease[2][1], LacY[2][1] , ECK0340, JW0334, b0343

Product description

LacY lactose MFS transporter[2][3]

Lactose permease; galactoside permease[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005887

integral to plasma membrane

PMID:7028742[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0015528

lactose:hydrogen symporter activity

PMID:3520229[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0015528

lactose:hydrogen symporter activity

PMID:7028742[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0015767

lactose transport

PMID:3520229[6]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0015767

lactose transport

PMID:7028742[5]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:4900505[7]

IDA: Inferred from Direct Assay

C

complete

GO:0030395

lactose binding

PMID:12893935[8]

IDA: Inferred from Direct Assay

F

complete

GO:0016021

integral to membrane

PMID:12893935[8]

IDA: Inferred from Direct Assay

C

complete

GO:0008643

carbohydrate transport

PMID:5541536[9]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005351

sugar:hydrogen symporter activity

PMID:7000781[10]

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 10 predicted transmembrane domains

Daley et al. (2005) [11]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MYYLKNTNFW MFGLFFFFYF FIMGAYFPFF PIWLHDINHI SKSDTGIIFA AISLFSLLFQ
PLFGLLSDKL GLRKYLLWII TGMLVMFAPF FIFIFGPLLQ YNILVGSIVG GIYLGFCFNA
GAPAVEAFIE KVSRRSNFEF GRARMFGCVG WALCASIVGI MFTINNQFVF WLGSGCALIL
AVLLFFAKTD APSSATVANA VGANHSAFSL KLALELFRQP KLWFLSLYVI GVSCTYDVFD
QQFANFFTSF FATGEQGTRV FGYVTTMGEL LNASIMFFAP LIINRIGGKN ALLLAGTIMS
VRIIGSSFAT SALEVVILKT LHMFEVPFLL VGCFKYITSQ FEVRFSATIY LVCFCFFKQL
AMIFMSVLAG NMYESIGFQG AYLVLGLVAL GFTLISVFTL SGPGPLSLLR RQVNEVA
Length

417

Mol. Wt

46.505 kDa

pI

8.9 (calculated)

Extinction coefficient

53,860 - 54,860 (calc based on 14 Y, 6 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..413

PF01306 LacY proton/sugar symporter

PMID:19920124[12]

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tmhmm.php?gene=lacY&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lacY taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128328

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949083

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001181

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P02920

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10526

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10526

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949083

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000519

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0521

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Newman, MJ et al. (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. J. Biol. Chem. 256 11804-8 PubMed
  6. ↑ 6.0 6.1 Viitanen, P et al. (1986) Purification, reconstitution, and characterization of the lac permease of Escherichia coli. Meth. Enzymol. 125 429-52 PubMed
  7. ↑ Jones, TH & Kennedy, EP (1969) Characterization of the membrane protein component of the lactose transport system of Escherichia coli. J. Biol. Chem. 244 5981-7 PubMed
  8. ↑ 8.0 8.1 Abramson, J et al. (2003) Structure and mechanism of the lactose permease of Escherichia coli. Science 301 610-5 PubMed
  9. ↑ Crandall, M & Koch, AL (1971) Temperature-sensitive mutants of Escherichia coli affecting beta-galactoside transport. J. Bacteriol. 105 609-19 PubMed
  10. ↑ Newman, MJ & Wilson, TH (1980) Solubilization and reconstitution of the lactose transport system from Escherichia coli. J. Biol. Chem. 255 10583-6 PubMed
  11. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  12. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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