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Expression|

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Standard Name	lacI
Gene Synonym(s)	ECK0342, b0345, JW0336^[1], JW0336
Product Desc.	LacI transcriptional repressor^[2]^[3] Transcriptional repressor of lac operon^[4]
Product Synonyms(s)	DNA-binding transcriptional repressor^[1], B0345^[2]^[1], LacI^[2]^[1], transcriptional repressor of the lac operon^[2]^[1] , ECK0342, JW0336, b0345
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): lacI^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lacI
Mnemonic	Lactose
Synonyms	ECK0342, b0345, JW0336^[1], JW0336
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	7.88 minutes	MG1655: 366734..365652 <gbrowseImage> name=NC_000913:365652..366734 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 340066..338984 <gbrowseImage> name=NC_012967:338984..340066 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 366734..365652 <gbrowseImage> name=NC_007779:365652..366734 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1386177..1385095 <gbrowseImage> name=NC_010473:1385095..1386177 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	365652	Edman degradation	PMID:1107032 PMID:4554534 PMID:4557395 PMID:4571224
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
lacI(del) (Keio:JW0336)	deletion	deletion		PMID:16738554	Shigen CGSC8528^[5]
lacIY282D		Y282D	(in T41 mutant)			Strain variation; seeded from UniProt:P03023
lacIY17H		Y17H	Broadening of specificity			seeded from UniProt:P03023
lacIR22N		R22N	Recognizes an operator variant			seeded from UniProt:P03023
lacI3042::Tn10	Insertion at 365,945 bp in MG1655 (NC_000913)			adapted from Nichols et al.^[6]	CAG18439 = CGSC7334^[5]	Synonyms: lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
lacI60					CGSC:5329
lacI22					CGSC:5462
lacI694(I^S)					CGSC:7293
lacI203					CGSC:7410
lacI100					CGSC:9476
lacI4500::Tn10					CGSC:24666
lacI3098::Tn10kan				PMID:2540407	CGSC:38617
lacI3042::Tn10				PMID:2540407	CGSC:39001
lacI373					CGSC:74371
lacI50(Fs)					CGSC:83700	frameshift mutation
lacI785(del)::kan				PMID:16738554	CGSC:101634
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0336	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACCAGTAACGTTATACGA Primer 2:CCCTGCCCGCTTTCCAGTCGGGA
10A6	Kohara Phage	Genobase	PMID:3038334
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: % ^[6]
mhpC281::Tn10	Linked marker	CAG12080 = CGSC7333^[5]	est. P1 cotransduction: 83% ^[6] Synonyms:zah-281::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10525	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10525	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000518	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945007	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0520	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LacI
Synonyms	DNA-binding transcriptional repressor^[1], B0345^[2]^[1], LacI^[2]^[1], transcriptional repressor of the lac operon^[2]^[1] , ECK0342, JW0336, b0345
Product description	LacI transcriptional repressor^[2]^[3] Transcriptional repressor of lac operon^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010982	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	lacZ		PMID:16606699	Experiment(s):EBI-1136310
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ
Length	360
Mol. Wt	38.589 kDa
pI	6.9 (calculated)
Extinction coefficient	22,920 - 23,295 (calc based on 8 Y, 2 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	5..50	PF00356 Bacterial regulatory proteins, lacI family	PMID:19920124
Domain	76..327	PF00532 family	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lacI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176012	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945007	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001189	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P03023	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10525	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10525	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945007	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000518	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0520	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein		10^-8	M	Medium:		PMID:16591435
Protein	E. coli K-12 MG1655	265	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	129	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	177	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
lacI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:366714..366754 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0345 (EcoliWiki Page)	NCBI GEO profiles for lacI
microarray	EcoliGenExpDB:b0345 (EcoliWiki Page)	Summary of data for lacI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (366635..366852) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:H2^[7]
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Notes

Accessions Related to lacI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10525	Escherichia coli str. K-12 substr. MG1655
EcoCyc (EcoliWiki Page)	EcoCyc:EG10525
EchoBASE	EchoBASE:EB0520	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b0345
EcoGene	EcoGene:EG10525	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000518	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000034998 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	LACI	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00356 Bacterial regulatory proteins, lacI family
Pfam (EcoliWiki Page)	PF00532 family
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

lacI:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lacI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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