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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	ilvE
Gene Synonym(s)	ECK3762, b3770, JW5606, ilvJ^[1]
Product Desc.	IlvE^[2]^[3]; Component of branched-chain amino-acid aminotransferase^[2]^[3] Branched-chain amino acid aminotransferase; transaminase B; dimer of trimers^[4]
Product Synonyms(s)	branched-chain amino-acid aminotransferase^[1], B3770^[2]^[1], IlvE^[2]^[1] , ECK3762, JW5606, b3770
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ilvEDA^[2], ilvLG_1G_2MEDA^[2], ilvLGGMEDA, ilvLGMEDA, ilvGMEDA
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Two independent determinations found N-terminal Gly where a Thr is encoded, indicating a modification of Thr to Gly may have occured post-translationally.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ilvE
Mnemonic	Isoleucine-valine (requirement)
Synonyms	ECK3762, b3770, JW5606, ilvJ^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	85.15 minutes	MG1655: 3950507..3951436 <gbrowseImage> name=NC_000913:3950507..3951436 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3914286..3915215 <gbrowseImage> name=NC_012967:3914286..3915215 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3840176..3841105 <gbrowseImage> name=NC_012759:3840176..3841105 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3684197..3683268 <gbrowseImage> name=NC_007779:3683268..3684197 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4049427..4050356 <gbrowseImage> name=NC_010473:4049427..4050356 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3950510	Edman degradation	PMID:378964 PMID:385001 PMID:3069843
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔilvE (Keio:JW5606)	deletion	deletion	PMID:16738554	Shigen CGSC11592^[5]
ilvE720::Tn5			PMID:395018	CGSC:5029
ilvE12			PMID:15983	CGSC:5977
ilvE164				CGSC:8362
ilvE316(Am)			PMID:4892370	CGSC:11349	amber (UAG) mutation
ΔilvE721::kan			PMID:16738554	CGSC:111837
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5606	Plasmid clone	Shigen	PMID:16769691 Status:cloned as old JW Primer 1:GCCACCACGAAGAAAGCTGATTA Primer 2:CCTTGATTAACTTGATCTAACCA
2A8	Kohara Phage	Genobase	PMID:3038334
2E6	Kohara Phage	Genobase	PMID:3038334
15D10	Kohara Phage	Genobase	PMID:3038334
rbsD296::Tn10	Linked marker	CAG18501 = CGSC7460^[5]	est. P1 cotransduction: 47% ^[6] Synonyms:zie-296::Tn10
ilvD500::Tn10	Linked marker	CAG18431 = CGSC7462^[5]	est. P1 cotransduction: 92% ^[6] Synonyms:ilv500::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10497	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10497	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000490	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948278	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0492	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012316	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	IlvE
Synonyms	branched-chain amino-acid aminotransferase^[1], B3770^[2]^[1], IlvE^[2]^[1] , ECK3762, JW5606, b3770
Product description	IlvE^[2]^[3]; Component of branched-chain amino-acid aminotransferase^[2]^[3] Branched-chain amino acid aminotransferase; transaminase B; dimer of trimers^[4]
EC number (for enzymes)	2.6.1.42^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001544	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018300	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004084	branched-chain-amino-acid transaminase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005785	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006532	aspartate biosynthetic process	PMID:15983	IGI: Inferred from Genetic Interaction	EcoliWiki:aspC\|EcoliWiki:tyrB	P		complete
	GO:0004084	branched-chain-amino-acid transaminase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.6.1.42	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008483	transaminase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0032	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009081	branched chain family amino acid metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005785	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009082	branched chain family amino acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0100	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042802	identical protein binding	PMID:378964	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of branched-chain amino-acid aminotransferase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTTKKADYIW FNGEMVRWED AKVHVMSHAL HYGTSVFEGI RCYDSHKGPV VFRHREHMQR LHDSAKIYRF PVSQSIDELM EACRDVIRKN NLTSAYIRPL IFVGDVGMGV NPPAGYSTDV IIAAFPWGAY LGAEALEQGI DAMVSSWNRA APNTIPTAAK AGGNYLSSLL VGSEARRHGY QEGIALDVNG YISEGAGENL FEVKDGVLFT PPFTSSALPG ITRDAIIKLA KELGIEVREQ VLSRESLYLA DEVFMSGTAA EITPVRSVDG IQVGEGRCGP VTKRIQQAFF GLFTGETEDK WGWLDQVNQ
Length	309
Mol. Wt	34.093 kDa
pI	5.7 (calculated)
Extinction coefficient	49,390 - 49,765 (calc based on 11 Y, 6 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0AB80
Domain	52..289	PF01063 Aminotransferase class IV		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ilvE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176403	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948278	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012316	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AB80	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10497	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10497	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948278	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000490	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0492	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	4.53E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	2072	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2671	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2636	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ilvEDA
ilvLG_1G_2MEDA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3950487..3950527 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3770 (EcoliWiki Page)	NCBI GEO profiles for ilvE
microarray	GenExpDB:b3770 (EcoliWiki Page)	Summary of data for ilvE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ilvE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10497	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0492	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3770
EcoGene	EcoGene:EG10497	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000490	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012316	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	ILVE	From SHIGELLACYC
E. coli O157	ILVE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01063 Aminotransferase class IV
Panther (EcoliWiki Page)	PTHR11825:SF19
Superfamily (EcoliWiki Page)	SUPERFAMILY:56752
EcoCyc	EcoCyc:EG10497	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10497	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000490	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0492	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012316	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 ^2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ilvE:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ilvE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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