ilvE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ilvE |
|---|---|
| Mnemonic |
Isoleucine-valine (requirement) |
| Synonyms |
ECK3762, b3770, JW5606, ilvJ[1] |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
85.15 minutes |
MG1655: 3950507..3951436 |
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NC_012967: 3914286..3915215 |
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NC_012759: 3840176..3841105 |
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W3110 |
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W3110: 3684197..3683268 |
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DH10B: 4049427..4050356 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3950510 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔilvE (Keio:JW5606) |
deletion |
deletion |
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ilvE720::Tn5 |
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ilvE12 |
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ilvE164 |
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ilvE316(Am) |
amber (UAG) mutation | ||||||
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ΔilvE721::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW5606 |
Plasmid clone |
Status:cloned as old JW Primer 1:GCCACCACGAAGAAAGCTGATTA Primer 2:CCTTGATTAACTTGATCTAACCA | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 47% [12] | ||
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Linked marker |
est. P1 cotransduction: 92% [12] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Lee-Peng, FC et al. (1979) Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity. J. Bacteriol. 139 339-45 PubMed
- ↑ Adams, CW et al. (1979) The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity. Biochem. Biophys. Res. Commun. 89 650-8 PubMed
- ↑ Inoue, K et al. (1988) Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties. J. Biochem. 104 777-84 PubMed
- ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ Shaw, KJ & Berg, CM (1979) Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics 92 741-7 PubMed
- ↑ Gelfand, DH & Steinberg, RA (1977) Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J. Bacteriol. 130 429-40 PubMed
- ↑ Wechsler, JA & Adelberg, EA (1969) Antipolarity in the ilv operon of Escherichia coli K-12. J. Bacteriol. 98 1179-94 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 11.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
