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Gene |

Product(s) |

Expression|

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Standard Name	fliC
Gene Synonym(s)	ECK1922, b1923, JW1908, flaF, hag, H^[1], H
Product Desc.	flagellar biosynthesis; flagellin, filament structural protein^[2]^[3]; Component of Flagellum^[3] Flagellin, structural gene, H-antigen^[4]
Product Synonyms(s)	flagellar filament structural protein (flagellin)^[1], B1923^[2]^[1], Hag^[2]^[1], FlaF^[2]^[1], FliC^[2]^[1] , ECK1922, flaF, H, hag, JW1908, b1923
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fliC^[2]
Regulation/Activity
Quick Links
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Notes

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Flagellar regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fliC
Mnemonic	Flagella
Synonyms	ECK1922, b1923, JW1908, flaF, hag, H^[1], H
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	43.11 minutes	MG1655: 2001630..2000134 <gbrowseImage> name=NC_000913:2000134..2001630 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1892617..1894113 <gbrowseImage> name=NC_012759:1892617..1894113 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2005743..2004247 <gbrowseImage> name=NC_007779:2004247..2005743 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2000137	Edman degradation	PMID:9119048 PMID:9298646 PMID:9600841
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
Strain DH10B		S111A	Nonsynonomous mutation	PMID:18245285	ASAP	This is a SNP in E. coli K-12 Strain DH10B
Strain DH10B		A49S	Nonsynonomous mutation	PMID:18245285	ASAP	This is a SNP in E. coli K-12 Strain DH10B
ΔfliC (Keio:JW1908)	deletion	deletion		PMID:16738554	Shigen CGSC9586^[5]
fliC::Tn5KAN-2 (FB20528)	Insertion at nt 342 in Minus orientation			PMID:15262929	E. coli Genome Project:FB20528	contains pKD46
fliC::Tn5KAN-2 (FB20529)	Insertion at nt 342 in Minus orientation			PMID:15262929	E. coli Genome Project:FB20529	does not contain pKD46
fliC585					CGSC:11708
fliC5303::Tn10				PMID:6993282	CGSC:63629
ΔfliC769::kan				PMID:16738554	CGSC:100930
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1908	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCACAAGTCATTAATACCAA Primer 2:CCACCCTGCAGCAGAGACAGAAC
16B12	Kohara Phage	Genobase	PMID:3038334
4B9	Kohara Phage	Genobase	PMID:3038334
uvrC279::Tn10	Linked marker	CAG12156 = CGSC7394^[5]	est. P1 cotransduction: 72% ^[6]
zed-3069::Tn10	Linked marker	CAG18451 = CGSC7395^[5]	est. P1 cotransduction: 22% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10321	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10321	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000315	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949101	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0317	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006402	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FliC
Synonyms	flagellar filament structural protein (flagellin)^[1], B1923^[2]^[1], Hag^[2]^[1], FlaF^[2]^[1], FliC^[2]^[1] , ECK1922, flaF, H, hag, JW1908, b1923
Product description	flagellar biosynthesis; flagellin, filament structural protein^[2]^[3]; Component of Flagellum^[3] Flagellin, structural gene, H-antigen^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0001539	ciliary or flagellar motility	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001029	P	Seeded from EcoCyc (v14.0)	complete
	GO:0001539	ciliary or flagellar motility	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001492	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005198	structural molecule activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001029	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005198	structural molecule activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001492	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005576	extracellular region	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0964	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005576	extracellular region	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0243	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001029	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0975	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0307	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009420	bacterial-type flagellum filament	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001492	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Flagellum	could be indirect
Protein	proS		PMID:16606699	Experiment(s):EBI-1141359
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1141359
Protein	bioC		PMID:16606699	Experiment(s):EBI-1141359
Protein	nadE		PMID:16606699	Experiment(s):EBI-1141359
Protein	aspS		PMID:16606699	Experiment(s):EBI-1141359
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Extracellular		PMID:12730325	EchoLocation:fliC
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAQVINTNSL SLITQNNINK NQSALSSSIE RLSSGLRINS AKDDAAGQAI ANRFTSNIKG LTQAARNAND GISVAQTTEG ALSEINNNLQ RVRELTVQAT TGTNSESDLS SIQDEIKSRL DEIDRVSGQT QFNGVNVLAK NGSMKIQVGA NDNQTITIDL KQIDAKTLGL DGFSVKNNDT VTTSAPVTAF GATTTNNIKL TGITLSTEAA TDTGGTNPAS IEGVYTDNGN DYYAKITGGD NDGKYYAVTV ANDGTVTMAT GATANATVTD ANTTKATTIT SGGTPVQIDN TAGSATANLG AVSLVKLQDS KGNDTDTYAL KDTNGNLYAA DVNETTGAVS VKTITYTDSS GAASSPTAVK LGGDDGKTEV VDIDGKTYDS ADLNGGNLQT GLTAGGEALT AVANGKTTDP LKALDDAIAS VDKFRSSLGA VQNRLDSAVT NLNNTTTNLS EAQSRIQDAD YATEVSNMSK AQIIQQAGNS VLAKANQVPQ QVLSLLQG
Length	498
Mol. Wt	51.295 kDa
pI	4.3 (calculated)
Extinction coefficient	14,900 (calc based on 10 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P04949
Domain	411..496	PF00700 Bacterial flagellin C-terminal helical region		PMID:19920124
Domain	5..143	PF00669 Bacterial flagellin N-terminal helical region		PMID:19920124
Domain	193..283	PF08884 Flagellin D3 domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fliC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129870	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949101	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006402	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P04949	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10321	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10321	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949101	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000315	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0317	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	75	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	157	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	9^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fliC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2001610..2001650 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1923 (EcoliWiki Page)	NCBI GEO profiles for fliC
microarray	GenExpDB:b1923 (EcoliWiki Page)	Summary of data for fliC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2001552..2001915) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ01; Well:H10^[7]
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Notes

Accessions Related to fliC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10321	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0317	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1923
EcoGene	EcoGene:EG10321	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000315	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006402	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000022061 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000043273 (score: 1.000; bootstrap: 82%) ENSXETP00000043230 (score: 0.543)	From Inparanoid:20070104
Shigella flexneri	FLIC	From SHIGELLACYC
E. coli O157	FLIC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00700 Bacterial flagellin C-terminal helical region
Pfam (EcoliWiki Page)	PF00669 Bacterial flagellin N-terminal helical region
Superfamily (EcoliWiki Page)	SUPERFAMILY:64518
Pfam (EcoliWiki Page)	PF08884 Flagellin D3 domain
EcoCyc	EcoCyc:EG10321	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10321	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000315	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0317	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006402	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

fliC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fliC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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