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Standard Name	fepA
Gene Synonym(s)	ECK0576, b0584, JW5086, cbr, cbt, fep, feuB, G399^[1]^[2]
Product Desc.	FepA, outer membrane receptor for ferric enterobactin (enterochelin) and colicins B and D^[2]^[3]; Component of Outer Membrane Ferric Enterobactin Transport System^[3]; Ferric Enterobactin Transport System^[3] Ferrienterobactin outer membrane receptor^[4]
Product Synonyms(s)	iron-enterobactin outer membrane transporter^[1], B0584^[2]^[1], Fep^[2]^[1], Cbt^[2]^[1], Cbr^[2]^[1], FeuB^[2]^[1], FepA^[2]^[1] , cbr, cbt, ECK0576, fep, feuB, JW5086, b0584
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fepA-entD^[2], OP00224, fepA
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fepA
Mnemonic	Ferrienterobactin permease
Synonyms	ECK0576, b0584, JW5086, cbr, cbt, fep, feuB, G399^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	13.14 minutes	MG1655: 611717..609477 <gbrowseImage> name=NC_000913:609477..611717 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 595201..592961 <gbrowseImage> name=NC_012967:592961..595201 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 512237..514477 <gbrowseImage> name=NC_012759:512237..514477 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 611717..609477 <gbrowseImage> name=NC_007779:609477..611717 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 551049..548809 <gbrowseImage> name=NC_010473:548809..551049 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 664309..662069 <gbrowseImage> name=NC_010473:662069..664309 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	609480	Edman degradation	PMID:10529396
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔfepA (Keio:JW5086)	deletion	deletion			PMID:16738554	Shigen CGSC11165^[5]
ΔfepA::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔfepA::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
ΔfepA::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
fepA9						CGSC:6702
ΔfepA721::kan					PMID:16738554	CGSC:100836
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5086	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACAAGAAGATTCATTCCCT Primer 2:CCGAAGTGGGTGTTTACGCTCAT
21A9S	Kohara Phage	Genobase	PMID:3038334
2D12	Kohara Phage	Genobase	PMID:3038334
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 6% ^[6] Synonyms:purE79::Tn10
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: 37% ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10293	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10293	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000287	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945193	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0289	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002014	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FepA
Synonyms	iron-enterobactin outer membrane transporter^[1], B0584^[2]^[1], Fep^[2]^[1], Cbt^[2]^[1], Cbr^[2]^[1], FeuB^[2]^[1], FepA^[2]^[1] , cbr, cbt, ECK0576, fep, feuB, JW5086, b0584
Product description	FepA, outer membrane receptor for ferric enterobactin (enterochelin) and colicins B and D^[2]^[3]; Component of Outer Membrane Ferric Enterobactin Transport System^[3]; Ferric Enterobactin Transport System^[3] Ferrienterobactin outer membrane receptor^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of Outer Membrane Ferric Enterobactin Transport System	could be indirect
Protein	Subunits of Ferric Enterobactin Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
outer membrane		From EcoCyc^[3]
Outer Membrane	PMID:10529396	EchoLocation:fepA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV STITADEIRK NPVARDVSKI IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG PENTLILIDG KPVSSRNSVR QGWRGERDTR GDTSWVPPEM IERIEVLRGP AAARYGNGAA GGVVNIITKK GSGEWHGSWD AYFNAPEHKE EGATKRTNFS LTGPLGDEFS FRLYGNLDKT QADAWDINQG HQSARAGTYA TTLPAGREGV INKDINGVVR WDFAPLQSLE LEAGYSRQGN LYAGDTQNTN SDSYTRSKYG DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK FNEKATQDFV DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS SNTQALTGTN TGGAIDGVST TDRSPYSKAE IFSLFAENNM ELTDSTIVTP GLRFDHHSIV GNNWSPALNI SQGLGDDFTL KMGIARAYKA PSLYQTNPNY ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR DGWLAGVTWF RNDYRNKIEA GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV MWTNNITYML KSENKTTGDR LSIIPEYTLN STLSWQARED LSMQTTFTWY GKQQPKKYNY KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL FDKRLWRAGN AQTTGDLAGA NYIAGAGAYT YNEPGRTWYM SVNTHF
Length	746
Mol. Wt	82.107 kDa
pI	5.3 (calculated)
Extinction coefficient	157,680 - 157,930 (calc based on 32 Y, 20 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P05825
Domain	465..745	PF00593 TonB dependent receptor		PMID:19920124
Domain	43..163	PF07715 TonB-dependent Receptor Plug Domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fepA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128567	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945193	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002014	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P05825	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10293	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10293	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945193	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000287	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0289	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.95E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	69	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	32	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	71	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fepA-entD
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:611697..611737 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0584 (EcoliWiki Page)	NCBI GEO profiles for fepA
microarray	GenExpDB:b0584 (EcoliWiki Page)	Summary of data for fepA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (611640..612087) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ16; Well:G2^[7]
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Notes

Accessions Related to fepA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10293	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0289	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0584
EcoGene	EcoGene:EG10293	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000287	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002014	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FEPA	From SHIGELLACYC
E. coli O157	FEPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:56935
Pfam (EcoliWiki Page)	PF00593 TonB dependent receptor
Pfam (EcoliWiki Page)	PF07715 TonB-dependent Receptor Plug Domain
EcoCyc	EcoCyc:EG10293	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10293	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000287	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0289	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002014	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

fepA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fepA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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