dsbG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dsbG

Mnemonic

Disulfide bond

Synonyms

ECK0598, b0604, JW0597, ybdP[1], ybdP

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.73 minutes 

MG1655: 637796..637050
<gbrowseImage> name=NC_000913:637050..637796 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 620624..619863
<gbrowseImage> name=NC_012967:619863..620624 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 539810..540556
<gbrowseImage> name=NC_012759:539810..540556 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 637796..637050
<gbrowseImage> name=NC_007779:637050..637796 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 577128..576382
<gbrowseImage> name=NC_010473:576382..577128 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 690388..689642
<gbrowseImage> name=NC_010473:689642..690388 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

637053

Edman degradation

PMID:9383195[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdsbG (Keio:JW0597)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8321[4]

dsbGC129A

C129A

Partial loss of redox activity

seeded from UniProt:P77202

dsbGC129S

C129S

No loss of chaperone activity; when associated with S-126

seeded from UniProt:P77202

dsbGC126A

C126A

Complete loss of redox activity

seeded from UniProt:P77202

dsbGC126S

C126S

No loss of chaperone activity; when associated with S-129

seeded from UniProt:P77202

dsbG601::Tn10

Insertion at 637,765 bp in MG1655 (NC_000913)

adapted from Nichols et al.[5]

CAG12149 = CGSC7346[4]

Synonyms: zbd-601::Tn10, zbe-601::Tn10

dsbG601::Tn10

PMID:2540407[6] PMID:9829956[7]

CGSC:5072

ΔdsbG743::kan

PMID:16738554[3]

CGSC:100654


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0597

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTTAAAAAAGATACTTTTACT

Primer 2:CCTTTATTCCCCATAATGATATT

4A5

Kohara Phage

Genobase

PMID:3038334[9]

3G5

Kohara Phage

Genobase

PMID:3038334[9]

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[4]

est. P1 cotransduction: % [5]
Synonyms:purE79::Tn10

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[4]

est. P1 cotransduction: 54% [5]
Synonyms:zbe-280::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6333

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13535

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002940

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945224

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3306

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002084

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Andersen, CL et al. (1997) A new Escherichia coli gene, dsbG, encodes a periplasmic protein involved in disulphide bond formation, required for recycling DsbA/DsbB and DsbC redox proteins. Mol. Microbiol. 26 121-32 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 5.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  7. Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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