fepA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fepA

Mnemonic

Ferrienterobactin permease

Synonyms

ECK0576, b0584, JW5086, cbr, cbt, fep, feuB, G399[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.14 minutes 

MG1655: 611717..609477
<gbrowseImage> name=NC_000913:609477..611717 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 595201..592961
<gbrowseImage> name=NC_012967:592961..595201 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 512237..514477
<gbrowseImage> name=NC_012759:512237..514477 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 611717..609477
<gbrowseImage> name=NC_007779:609477..611717 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 551049..548809
<gbrowseImage> name=NC_010473:548809..551049 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 664309..662069
<gbrowseImage> name=NC_010473:662069..664309 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

609480

Edman degradation

PMID:10529396[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔfepA (Keio:JW5086)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC11165[5]

ΔfepA::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[6]

ΔfepA::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[6]

ΔfepA::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[6]

fepA9

CGSC:6702

ΔfepA721::kan

PMID:16738554[4]

CGSC:100836


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5086

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAACAAGAAGATTCATTCCCT

Primer 2:CCGAAGTGGGTGTTTACGCTCAT

21A9S

Kohara Phage

Genobase

PMID:3038334[8]

2D12

Kohara Phage

Genobase

PMID:3038334[8]

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[5]

est. P1 cotransduction: 6% [9]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[5]

est. P1 cotransduction: 37% [9]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10293

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10293

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000287

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945193

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0289

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002014

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Wachi, M et al. (1999) Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant. Biochem. Biophys. Res. Commun. 264 525-9 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 6.2 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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