{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	eda
Gene Synonym(s)	ECK1851, b1850, JW1839, hga, kdgA, kga^[1], kga
Product Desc.	Eda^[2]^[3]; Component of KDPGALDOL-4OH2OXOGLUTARALDOL-CPLX^[2] KHG/KDPG aldolase; 2-keto-4-hydroxyglutarate aldolase and 2-keto-3-deoxy-6-phosphogluconate aldolase activities; homotrimeric; 2-keto-4-hydroxyglutarate aldolase is also known as 4-hydroxy-2-oxoglutarate aldolase. 2-keto-3-deoxy-6-phosphogluconate aldolas^[4]
Product Synonyms(s)	multifunctional 2-keto-3-deoxygluconate 6-phosphate aldolase and 2-keto-4-hydroxyglutarate aldolase and oxaloacetate decarboxylase^[1], B1850^[2]^[1], Kga^[2]^[1], Hga^[2]^[1], KdgA^[2]^[1], Eda^[2]^[1] , ECK1851, hga, JW1839, kdgA, kga, b1850
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): eda^[2], edd-eda^[2], OP00322
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	eda
Mnemonic	Entner-Douderoff aldolase
Synonyms	ECK1851, b1850, JW1839, hga, kdgA, kga^[1], kga
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	41.6 minutes	MG1655: 1930780..1930139 <gbrowseImage> name=NC_000913:1930139..1930780 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1911332..1910691 <gbrowseImage> name=NC_012967:1910691..1911332 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1822198..1822839 <gbrowseImage> name=NC_012759:1822198..1822839 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1934470..1933829 <gbrowseImage> name=NC_007779:1933829..1934470 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2021351..2020710 <gbrowseImage> name=NC_010473:2020710..2021351 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1930139	Edman degradation	PMID:3136164
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
eda(del) (Keio:JW1839)	deletion	deletion			PMID:16738554	Shigen CGSC9535^[5]
eda::Tn5KAN-2 (FB20471)	Insertion at nt 308 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20471	contains pKD46
eda-51::Tn10	Insertion at 1,930,416 bp in MG1655 (NC_000913)				adapted from Nichols et al.^[6]	CAG18486 = CGSC7392^[5]	Synonyms:
eda-51::Tn10						CGSC:5058
eda-1					PMID:4560065	CGSC:8057
eda-50						CGSC:9052
eda-10						CGSC:11702
eda-3126::Tn10kan					PMID:2540407	CGSC:39984
eda-775(del)::kan					PMID:16738554	CGSC:100684
eda::miniTn10::npt Str^r			in vivo	Mutants were unable to colonize mouse intestine to any great extent when fed alone to mice, Fig 3. However, when eda mutants were complemented with a wild-type eda gene, their colonizing ability was restored, fig 5.	PMID:8751891
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1839	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAAACTGGAAAACAAGTGC Primer 2:CCaAGCTTAGCGCCTTCTACAGC
zea-225::Tn10	Linked marker	CAG18465 = CGSC7388^[5]	est. P1 cotransduction: 4% ^[6] Synonyms:zdj-225::Tn10
uvrC279::Tn10	Linked marker	CAG12156 = CGSC7394^[5]	est. P1 cotransduction: 4% ^[6]
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10256	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10256	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000250	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946367	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0252	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006164	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Eda
Synonyms	multifunctional 2-keto-3-deoxygluconate 6-phosphate aldolase and 2-keto-4-hydroxyglutarate aldolase and oxaloacetate decarboxylase^[1], B1850^[2]^[1], Kga^[2]^[1], Hga^[2]^[1], KdgA^[2]^[1], Eda^[2]^[1] , ECK1851, hga, JW1839, kdgA, kga, b1850
Product description	Eda^[2]^[3]; Component of KDPGALDOL-4OH2OXOGLUTARALDOL-CPLX^[2] KHG/KDPG aldolase; 2-keto-4-hydroxyglutarate aldolase and 2-keto-3-deoxy-6-phosphogluconate aldolase activities; homotrimeric; 2-keto-4-hydroxyglutarate aldolase is also known as 4-hydroxy-2-oxoglutarate aldolase. 2-keto-3-deoxy-6-phosphogluconate aldolas^[4]
EC number (for enzymes)	4.1.2.16^[1] 4.1.1.3^[1] 4.1.2.14^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000887	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008675	2-dehydro-3-deoxy-phosphogluconate aldolase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.2.14	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008675	2-dehydro-3-deoxy-phosphogluconate aldolase activity	PMID:4945194	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0008700	4-hydroxy-2-oxoglutarate aldolase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.3.16	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of KDPGALDOL-4OH2OXOGLUTARALDOL-CPLX	could be indirect
Protein	rplB		PMID:16606699	Experiment(s):EBI-1141014
Protein	groL		PMID:16606699	Experiment(s):EBI-1141014
Protein	ycgE		PMID:16606699	Experiment(s):EBI-1141014
Protein	btuD		PMID:16606699	Experiment(s):EBI-1141014
Protein	otsB		PMID:16606699	Experiment(s):EBI-1141014
Protein	lacZ		PMID:16606699	Experiment(s):EBI-1141014
Protein	rhsA		PMID:16606699	Experiment(s):EBI-1141014
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKNWKTSAES ILTTGPVVPV IVVKKLEHAV PMAKALVAGG VRVLEVTLRT ECAVDAIRAI AKEVPEAIVG AGTVLNPQQL AEVTEAGAQF AISPGLTEPL LKAATEGTIP LIPGISTVSE LMLGMDYGLK EFKFFPAEAN GGVKALQAIA GPFSQVRFCP TGGISPANYR DYLALKSVLC IGGSWLVPAD ALEAGDYDRI TKLAREAVEG AKL
Length	213
Mol. Wt	22.283 kDa
pI	5.5 (calculated)
Extinction coefficient	16,960 - 17,335 (calc based on 4 Y, 2 W, and 3 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	8..203	PF01081 KDPG and KHG aldolase		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=eda taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129803	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946367	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006164	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A955	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10256	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10256	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946367	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000250	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0252	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	6.92E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	11006	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	3295	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	8106	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
eda
edd-eda
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:1930760..1930800 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1850 (EcoliWiki Page)	NCBI GEO profiles for eda
microarray	GenExpDB:b1850 (EcoliWiki Page)	Summary of data for eda from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to eda Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10256	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0252	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1850
EcoGene	EcoGene:EG10256	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000250	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006164	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	EDA	From SHIGELLACYC
E. coli O157	EDA	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01081 KDPG and KHG aldolase
Superfamily (EcoliWiki Page)	SUPERFAMILY:51569
EcoCyc	EcoCyc:EG10256	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10256	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000250	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0252	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006164	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

eda:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to eda Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search