cyoA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
cyoA |
---|---|
Gene Synonym(s) |
ECK0426, b0432, JW0422[1], JW0422 |
Product Desc. |
cytochrome bo terminal oxidase subunit II[2][3]; Component of cytochrome bo terminal oxidase[2][3] Cytochrome o oxidase subunit II, lipoprotein; also called cytochrome bo(3) ubiquinol oxidase subunit II[4] |
Product Synonyms(s) |
cytochrome o ubiquinol oxidase subunit II[1], B0432[2][1], CyoA[2][1] , ECK0426, JW0422, b0432 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
2 IM TMS; cleavable signal peptide can be incorrectly predicted as a third TMS. First 24 aa are a type II signal peptide. First 25 aa and the last 200 aa of the mature protein are in the periplasm. Translocation of the N-terminal domain requires YidC; translocation of the large C-terminal domain requires both YidC and the Sec translocon, and is dependent upon prior translocation of the N-terminal domain.[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
cyoA |
---|---|
Mnemonic |
Cytochrome o oxidase |
Synonyms |
ECK0426, b0432, JW0422[1], JW0422 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
9.7 minutes |
MG1655: 450834..449887 |
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NC_012967: 422313..421366 |
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NC_012759: 352646..353593 |
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W3110 |
|
W3110: 450834..449887 |
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DH10B: 390165..389218 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
cyoA(del) (Keio:JW0422) |
deletion |
deletion |
PMID:16738554 |
||||
cyoA(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
PMID:16095938 |
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cyoA(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
PMID:16095938 |
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cyoA(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
PMID:16095938 |
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CydAB (del), cyoABCD (del), cbdAB (del), ygiN (del) |
deletion |
deletion |
deletion of all genes decreased oxygen uptake by 98% via activation of arcA, table 1. |
PMID:20709841 |
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cyoA789(del)::FRT |
deletion |
Mutagenesis Rate |
Decrease in stress induced mutagenesis (SIM). |
PMID:23224554 |
Parent Strain: SMR4562 Experimental Strain: SMR12570 |
The mutation conferred a significant decrease in SIM with mutant frequency being reduced by 68 to 89 percent. See table S3 for full experimental results. | |
cyoA789(del)::FRT |
deletion |
Sensitivity to |
SDS-EDTA Sensitivity |
PMID:23224554 |
Parent Strain: SMR4562 Experimental Strain: SMR12570 |
The mutation conferred an increase in SDS-EDTA sensitivity. See table S7 and S1 for full experimental data. | |
SMR4562 yiaG-yfp FRTcatFRT cyoA789(del)::FRTKanFRT |
Deletion & Insertion |
Sigma S activity |
Decrease in SigmaS activity |
PMID:23224554 |
Parental Strain: SMR10582 Experimental Strain: SMR14283 |
See table S8 for full experimental data. | |
CAG45114 cyoA789(del)::FRTKanFRT |
deletion |
SigmaE activity |
Decrease in SigmaE activity |
PMID:23224554 |
Parental Strain: CAG45114 Experimental Strain: SMR15330 |
See table S11 for full experimental results. | |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0422 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAGACTCAGGAAATACAATAA Primer 2:CCATGGGCGGATTCCGCGTGGCT | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 52% [6] | ||
Linked marker |
est. P1 cotransduction: 72% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000173 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0175 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0001495 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
CyoA |
---|---|
Synonyms |
cytochrome o ubiquinol oxidase subunit II[1], B0432[2][1], CyoA[2][1] , ECK0426, JW0422, b0432 |
Product description |
cytochrome bo terminal oxidase subunit II[2][3]; Component of cytochrome bo terminal oxidase[2][3] Cytochrome o oxidase subunit II, lipoprotein; also called cytochrome bo(3) ubiquinol oxidase subunit II[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0004129 |
cytochrome-c oxidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002429 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004129 |
cytochrome-c oxidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR011759 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004129 |
cytochrome-c oxidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR015964 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005488 |
binding |
PMID:7961841 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005507 |
copper ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002429 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005507 |
copper ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR011759 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005507 |
copper ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR015964 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0813 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009055 |
electron carrier activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR011759 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009055 |
electron carrier activity |
PMID:3301837 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0009060 |
aerobic respiration |
PMID:2981797 |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0009486 |
cytochrome bo3 ubiquinol oxidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR012141 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0015078 |
hydrogen ion transmembrane transporter activity |
PMID:2544445 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0015453 |
oxidoreduction-driven active transmembrane transporter activity |
PMID:7961841 |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0020037 |
heme binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR015964 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0022900 |
electron transport chain |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010514 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0022900 |
electron transport chain |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR011759 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0022900 |
electron transport chain |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0249 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005887 |
integral to plasma membrane |
PMID:2162836 |
IDA: Inferred from Direct Assay |
C |
complete | |||
GO:0022904 |
respiratory electron transport chain |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR015964 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006333 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010514 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR012141 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0560 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0070469 |
respiratory chain |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0679 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of [[:Category:Complex:cytochrome bo terminal oxidase|cytochrome bo terminal oxidase]] |
could be indirect |
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Protein |
kdpB |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
dadA |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
hflK |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
lon |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
groL |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
nagC |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
pflB |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
htpG |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
mukB |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
dinG |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
hslU |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
yoaA |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
dnaJ |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
helD |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
glpD |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
dnaK |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
galS |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
prs |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
ftsZ |
PMID:16606699 |
Experiment(s):EBI-1136570 | |
Protein |
rpsP |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
acpP |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
nusG |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
plasma membrane |
C-terminus localized to the periplasm with 3 predicted transmembrane domains |
Daley et al. (2005) [7] |
| |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MRLRKYNKSL GWLSLFAGTV LLSGCNSALL DPKGQIGLEQ RSLILTAFGL MLIVVIPAIL MAVGFAWKYR ASNKDAKYSP NWSHSNKVEA VVWTVPILII IFLAVLTWKT THALEPSKPL AHDEKPITIE VVSMDWKWFF IYPEQGIATV NEIAFPANTP VYFKVTSNSV MNSFFIPRLG SQIYAMAGMQ TRLHLIANEP GTYDGISASY SGPGFSGMKF KAIATPDRAA FDQWVAKAKQ SPNTMSDMAA FEKLAAPSEY NQVEYFSNVK PDLFADVINK FMAHGKSMDM TQPEGEHSAH EGMEGMDMSH AESAH |
Length |
315 |
Mol. Wt |
34.911 kDa |
pI |
7.3 (calculated) |
Extinction coefficient |
58,900 - 59,025 (calc based on 10 Y, 8 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0001495 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000173 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0175 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
8.77E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
E. coli K-12 MG1655 |
15769 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
3421 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
6446 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:450814..450854
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for cyoA | |
microarray |
Summary of data for cyoA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
GFP Fusion |
Intergenic region (450737..451303) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
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Notes
Accessions Related to cyoA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0175 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000173 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0001495 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Danio rerio |
|
From Inparanoid:20070104 |
Gallus gallus |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
E. coli O157 |
CYOA |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10178 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000173 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0175 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0001495 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
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