clpP:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

clpP

Mnemonic

Caseinolytic protease

Synonyms

ECK0431, b0437, JW0427, F21.5 gene, LopP, wseA[1], lopP, wseA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

9.83 minutes 

MG1655: 455901..456524
<gbrowseImage> name=NC_000913:455901..456524 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 427382..428005
<gbrowseImage> name=NC_012967:427382..428005 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 358660..359283
<gbrowseImage> name=NC_012759:358660..359283 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 455901..456524
<gbrowseImage> name=NC_007779:455901..456524 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 395232..395855
<gbrowseImage> name=NC_010473:395232..395855 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

455943

Edman degradation

PMID:2197275[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

clpP(del) (Keio:JW0427)

deletion

deletion

PMID:16738554[3]

Shigen

CGSC8590[4]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[5]

clpP(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[5]

clpP101(del-ins)::cat

PMID:2197275[2]

CGSC:90917

clpP723(del)::kan

PMID:16738554[3]

CGSC:100340


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0427

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCATACAGCGGCGAACGAGA

Primer 2:CCATTACGATGGGTCAGAATCGA

3B6

Kohara Phage

Genobase

PMID:3038334[7]

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[4]

est. P1 cotransduction: 40% [8]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[4]

est. P1 cotransduction: 89% [8]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10158

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10158

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000154

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945082

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0156

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001515

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Maurizi, MR et al. (1990) Sequence and structure of Clp P, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli. J. Biol. Chem. 265 12536-45 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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