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Expression|

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Standard Name	cheW
Gene Synonym(s)	ECK1888, b1887, JW1876^[1], JW1876
Product Desc.	CheW^[2]^[3] Chemotaxis signal transducer; bridges CheA to chemoreceptors to regulate phophotransfer to CheY and CheB^[4]
Product Synonyms(s)	purine-binding chemotaxis protein^[1], B1887^[2]^[1], CheW^[2]^[1] , ECK1888, JW1876, b1887
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): motAB-cheAW^[2]
Regulation/Activity
Quick Links
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Notes

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Flagellar regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cheW
Mnemonic	Chemotaxis
Synonyms	ECK1888, b1887, JW1876^[1], JW1876
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	42.48 minutes, 42.48 minutes	MG1655: 1971363..1970860 <gbrowseImage> name=NC_000913:1970860..1971363 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1952109..1951606 <gbrowseImage> name=NC_012967:1951606..1952109 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1868725..1869741 <gbrowseImage> name=NC_012759:1868725..1869741 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
BW2952		NC_012759: 1862919..1863422 <gbrowseImage> name=NC_012759:1862919..1863422 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1975053..1974550 <gbrowseImage> name=NC_007779:1974550..1975053 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2061934..2061431 <gbrowseImage> name=NC_010473:2061431..2061934 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔcheW (Keio:JW1876)	deletion	deletion	PMID:16738554	Shigen CGSC9562^[5]
cheW::Tn5KAN-2 (FB20492)	Insertion at nt 25 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20492	does not contain pKD46
cheW::Tn5KAN-2 (FB20493)	Insertion at nt 25 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20493	contains pKD46
ΔcheW740::kan			PMID:16738554	CGSC:100302
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1876	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACCGGTATGACGAATGTAAC Primer 2:CCgGCCACTTCTGACGCCGCGCT
12C7	Kohara Phage	Genobase	PMID:3038334
7F11	Kohara Phage	Genobase	PMID:3038334
eda-51::Tn10	Linked marker	CAG18486 = CGSC7392^[5]	est. P1 cotransduction: 18% ^[6]
uvrC279::Tn10	Linked marker	CAG12156 = CGSC7394^[5]	est. P1 cotransduction: 49% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10149	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10149	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000145	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946400	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0147	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006292	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CheW
Synonyms	purine-binding chemotaxis protein^[1], B1887^[2]^[1], CheW^[2]^[1] , ECK1888, JW1876, b1887
Product description	CheW^[2]^[3] Chemotaxis signal transducer; bridges CheA to chemoreceptors to regulate phophotransfer to CheY and CheB^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004871	signal transducer activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002545	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002545	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006935	chemotaxis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002545	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006935	chemotaxis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0145	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007165	signal transduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002545	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dadA	PMID:16606699	Experiment(s):EBI-1141189
Protein	leuA	PMID:16606699	Experiment(s):EBI-1141189
Protein	tsr	PMID:16606699	Experiment(s):EBI-1141189
Protein	torA	PMID:16606699	Experiment(s):EBI-1141189
Protein	yciA	PMID:16606699	Experiment(s):EBI-1141189
Protein	yfjJ	PMID:16606699	Experiment(s):EBI-1141189
Protein	atpD	PMID:16606699	Experiment(s):EBI-1141189
Protein	hflK	PMID:16606699	Experiment(s):EBI-1141189
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1141189
Protein	adk	PMID:16606699	Experiment(s):EBI-1141189
Protein	rplN	PMID:16606699	Experiment(s):EBI-1141189
Protein	bglX	PMID:16606699	Experiment(s):EBI-1141189
Protein	dnaJ	PMID:16606699	Experiment(s):EBI-1141189
Protein	clpX	PMID:16606699	Experiment(s):EBI-1141189
Protein	minD	PMID:16606699	Experiment(s):EBI-1141189
Protein	diaA	PMID:16606699	Experiment(s):EBI-1141189
Protein	insC1	PMID:16606699	Experiment(s):EBI-1141189
Protein	nadE	PMID:16606699	Experiment(s):EBI-1141189
Protein	ftsZ	PMID:16606699	Experiment(s):EBI-1141189
Protein	recA	PMID:16606699	Experiment(s):EBI-1141189
Protein	yfcF	PMID:16606699	Experiment(s):EBI-1141189
Protein	tar	PMID:16606699	Experiment(s):EBI-1141189
Protein	dacA	PMID:16606699	Experiment(s):EBI-1141189
Protein	mreB	PMID:16606699	Experiment(s):EBI-1141189
Protein	yhgF	PMID:16606699	Experiment(s):EBI-1141189
Protein	parE	PMID:19402753	MALDI(Z-score):24.795104
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTGMTNVTKL ASEPSGQEFL VFTLGDEEYG IDILKVQEIR GYDQVTRIAN TPAFIKGVTN LRGVIVPIVD LRIKFSQVDV DYNDNTVVIV LNLGQRVVGI VVDGVSDVLS LTAEQIRPAP EFAVTLSTEY LTGLGALGDR MLILVNIEKL LNSEEMALLD SAASEVA
Length	167
Mol. Wt	18.083 kDa
pI	4.2 (calculated)
Extinction coefficient	5,960 (calc based on 4 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	18..155	PF01584 CheW-like domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cheW taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129839	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946400	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006292	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A964	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10149	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10149	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946400	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000145	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0147	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.437+/-0.045	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	89	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	48	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	22^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
motAB-cheAW
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1971343..1971383 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1887 (EcoliWiki Page)	NCBI GEO profiles for cheW
microarray	GenExpDB:b1887 (EcoliWiki Page)	Summary of data for cheW from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to cheW Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10149	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0147	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1887
EcoGene	EcoGene:EG10149	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000145	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006292	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000073 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000004021 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	CHEW	From SHIGELLACYC
E. coli O157	CHEW	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01584 CheW-like domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:50341
EcoCyc	EcoCyc:EG10149	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10149	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000145	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0147	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006292	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

cheW:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cheW Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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