argR:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

argR

Gene Synonym(s)

ECK3226, b3237, JW3206, xerA, Rarg[1], Rarg

Product Desc.

ArgR[2][3];

Component of ArgR transcriptional dual regulator[2][3]; ArgR-L-arginine transcriptional dual regulator[3]

Repressor of Arg regulon; cer-mediated site-specific recombination in plasmid ColE1; hexameric[4]

Product Synonyms(s)

DNA-binding transcriptional dual regulator, L-arginine-binding[1], B3237[2][1], XerA[2][1], ArgR[2][1], repressor of arg regulon; cer-mediated site specific recombination[2][1] , ECK3226, JW3206, Rarg, xerA, b3237

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): argR[2], OP00009, TU00007, argR_carAB

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argR

Mnemonic

Arginine

Synonyms

ECK3226, b3237, JW3206, xerA, Rarg[1], Rarg

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.91 minutes 

MG1655: 3382725..3383195
<gbrowseImage> name=NC_000913:3382725..3383195 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3312600..3313070
<gbrowseImage> name=NC_012967:3312600..3313070 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3269873..3270343
<gbrowseImage> name=NC_012759:3269873..3270343 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3384558..3385028
<gbrowseImage> name=NC_007779:3384558..3385028 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3480470..3480940
<gbrowseImage> name=NC_010473:3480470..3480940 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3382725

Edman degradation

PMID:3116542


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

argRG123D

G123D

Auxotrophies

Defective binding to arginine and to ARG box. Forms dimers not hexamers

seeded from UniProt:P0A6D0

argRS44F

S44F

Auxotrophies

Defective binding to ARG box

seeded from UniProt:P0A6D0

argRS47L

S47L

Auxotrophies

Defective binding to ARG box

seeded from UniProt:P0A6D0

argRP76L

P76L

Auxotrophies

Increased affinity for ARG box in the absence of arginine

seeded from UniProt:P0A6D0

argRA105V

A105V

Auxotrophies

Defective binding to arginine and to ARG box

seeded from UniProt:P0A6D0

argRE21K

E21K

Auxotrophies

Increased affinity for ARG box in the presence of arginine

seeded from UniProt:P0A6D0

ΔargR (Keio:JW3206)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen CGSC10431[5]

argR::Tn5KAN-I-SceI (FB21082)

Insertion at nt 118 in Minus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB21082

contains pKD46

ΔargR::kan

deletion

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

argR38

Auxotrophies

CGSC:5153

argR40

Auxotrophies

CGSC:5155

argR63

Auxotrophies

CGSC:5179

argR64

Auxotrophies

CGSC:5180

argR84

Auxotrophies

CGSC:5200

[argR141]

Auxotrophies

CGSC:5209

null allele

argR142

Auxotrophies

CGSC:5210

ΔargR762::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:100054


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3206

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCGAAGCTCGGCTAAGCAAGA

Primer 2:CCAAGCTCCTGGTCGAACAGCTC

1E3

Kohara Phage

Genobase

PMID:3038334

3G9

Kohara Phage

Genobase

PMID:3038334

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[5]

est. P1 cotransduction: 50% [6]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[5]

est. P1 cotransduction: 28% [6]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10070

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10070

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000066

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947861

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0068

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010620

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArgR

Synonyms

DNA-binding transcriptional dual regulator, L-arginine-binding[1], B3237[2][1], XerA[2][1], ArgR[2][1], repressor of arg regulon; cer-mediated site specific recombination[2][1] , ECK3226, JW3206, Rarg, xerA, b3237

Product description

ArgR[2][3];

Component of ArgR transcriptional dual regulator[2][3]; ArgR-L-arginine transcriptional dual regulator[3]

Repressor of Arg regulon; cer-mediated site-specific recombination in plasmid ColE1; hexameric[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00173

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001669

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020899

F

Seeded from EcoCyc (v14.0)

complete

GO:0042150

plasmid recombination

PMID:3149585

IMP: Inferred from Mutant Phenotype

P

XerC, XerD, ArgR, and PepA, are required for site-specific recombination at the ColE1 cer locus in E. coli K-12 (see references in [7]).

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00173

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00173

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001669

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020899

P

Seeded from EcoCyc (v14.0)

complete

GO:0006525

arginine metabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00173

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0055

P

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of ArgR transcriptional dual regulator

could be indirect

Protein

aspC

PMID:16606699

Experiment(s):EBI-1145252

Protein

ydcD

PMID:16606699

Experiment(s):EBI-1145252

Protein

ribD

PMID:16606699

Experiment(s):EBI-1145252

Protein

dnaJ

PMID:16606699

Experiment(s):EBI-1145252

Protein

cysP

PMID:16606699

Experiment(s):EBI-1145252

Protein

Subunits of ArgR-L-arginine transcriptional dual regulator

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MRSSAKQEEL VKAFKALLKE EKFSSQGEIV AALQEQGFDN INQSKVSRML TKFGAVRTRN
AKMEMVYCLP AELGVPTTSS PLKNLVLDID YNDAVVVIHT SPGAAQLIAR LLDSLGKAEG
ILGTIAGDDT IFTTPANGFT VKDLYEAILE LFDQEL
Length

156

Mol. Wt

16.994 kDa

pI

4.8 (calculated)

Extinction coefficient

4,470 - 4,595 (calc based on 3 Y, 0 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

5..75

PF01316 Arginine repressor, DNA binding domain

PMID:19920124

Domain

81..151

PF02863 Arginine repressor, C-terminal domain

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=argR taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131127

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947861

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010620

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6D0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10070

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10070

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947861

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000066

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0068

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

3610

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1003

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1158

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

argR

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3382705..3382745 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3237 (EcoliWiki Page)

NCBI GEO profiles for argR

microarray

GenExpDB:b3237 (EcoliWiki Page)

Summary of data for argR from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to argR Expression

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Database Accession Notes

EcoCyc

EcoCyc:EG10070

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0068

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3237

EcoGene

EcoGene:EG10070

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000066

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010620

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000001361 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ARGR

From SHIGELLACYC

E. coli O157

ARGR

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02863 Arginine repressor, C-terminal domain

Pfam (EcoliWiki Page)

PF01316 Arginine repressor, DNA binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:46785

Superfamily (EcoliWiki Page)

SUPERFAMILY:55252

EcoCyc

EcoCyc:EG10070

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10070

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000066

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0068

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010620

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Sherratt, DJ et al. (1995) Site-specific recombination and circular chromosome segregation. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 347 37-42 PubMed

Categories

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