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Standard Name	aldA
Gene Synonym(s)	ECK1408, b1415, JW1412, ald^[1], ald
Product Desc.	AldA^[2]^[3]; Component of aldehyde dehydrogenase A, NAD-linked^[2]^[3] Aldehyde dehydrogenase, NAD-dependent; active on lactaldehyde, glycolaldehyde, and other aldehydes^[4]
Product Synonyms(s)	aldehyde dehydrogenase A, NAD-linked^[1], B1415^[2]^[1], Ald^[2]^[1], AldA^[2]^[1] , ald, ECK1408, JW1412, b1415
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aldA^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aldA
Mnemonic	Aldehyde dehydrogenase
Synonyms	ECK1408, b1415, JW1412, ald^[1], ald
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	32.03 minutes	MG1655: 1486256..1487695 <gbrowseImage> name=NC_000913:1486256..1487695 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1457563..1459002 <gbrowseImage> name=NC_012967:1457563..1459002 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1378315..1379754 <gbrowseImage> name=NC_012759:1378315..1379754 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1489946..1491385 <gbrowseImage> name=NC_007779:1489946..1491385 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1576851..1578290 <gbrowseImage> name=NC_010473:1576851..1578290 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1486259	Edman degradation	PMID:1917845 PMID:9298646 PMID:9732527
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔaldA (Keio:JW1412)	deletion	deletion	PMID:16738554	Shigen CGSC9239^[5]
aldA::Tn5KAN-2 (FB20313)	Insertion at nt 297 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20313	contains pKD46
ΔaldA776::kan			PMID:16738554	CGSC:99962
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1412	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCAGTACCCGTTCAACATCC Primer 2:CCAGACTGTAAATAAACCACCTG
3C9	Kohara Phage	Genobase	PMID:3038334
24D6	Kohara Phage	Genobase	PMID:3038334
zhj-3076::Tn10	Linked marker	CAG18640 = CGSC7453^[5]	est. P1 cotransduction: 8% ^[6]
trg-2::Tn10	Linked marker	CAG12026 = CGSC7376^[5]	est. P1 cotransduction: 77% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10035	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10035	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000032	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945672	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0034	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004725	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AldA
Synonyms	aldehyde dehydrogenase A, NAD-linked^[1], B1415^[2]^[1], Ald^[2]^[1], AldA^[2]^[1] , ald, ECK1408, JW1412, b1415
Product description	AldA^[2]^[3]; Component of aldehyde dehydrogenase A, NAD-linked^[2]^[3] Aldehyde dehydrogenase, NAD-dependent; active on lactaldehyde, glycolaldehyde, and other aldehydes^[4]
EC number (for enzymes)	1.2.1.22^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	Aspect	Notes	Status
	GO:0005737	cytoplasm			C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008911	lactaldehyde dehydrogenase activity	PMID:4310089	IDA: Inferred from Direct Assay	F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of aldehyde dehydrogenase A, NAD-linked	could be indirect
Protein	rhsA		PMID:16606699	Experiment(s):EBI-1139800
Protein	rhsC		PMID:16606699	Experiment(s):EBI-1139800
Protein	glcB		PMID:19402753	LCMS(ID Probability):99.6
Protein	edd		PMID:19402753	LCMS(ID Probability):99.6
Protein	fur		PMID:19402753	LCMS(ID Probability):99.6
Protein	acnB		PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):5.410000
Protein	gcvP		PMID:19402753	LCMS(ID Probability):99.6
Protein	gabD		PMID:19402753	LCMS(ID Probability):99.6
Protein	folD		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSVPVQHPMY IDGQFVTWRG DAWIDVVNPA TEAVISRIPD GQAEDARKAI DAAERAQPEW EALPAIERAS WLRKISAGIR ERASEISALI VEEGGKIQQL AEVEVAFTAD YIDYMAEWAR RYEGEIIQSD RPGENILLFK RALGVTTGIL PWNFPFFLIA RKMAPALLTG NTIVIKPSEF TPNNAIAFAK IVDEIGLPRG VFNLVLGRGE TVGQELAGNP KVAMVSMTGS VSAGEKIMAT AAKNITKVCL ELGGKAPAIV MDDADLELAV KAIVDSRVIN SGQVCNCAER VYVQKGIYDQ FVNRLGEAMQ AVQFGNPAER NDIAMGPLIN AAALERVEQK VARAVEEGAR VAFGGKAVEG KGYYYPPTLL LDVRQEMSIM HEETFGPVLP VVAFDTLEDA ISMANDSDYG LTSSIYTQNL NVAMKAIKGL KFGETYINRE NFEAMQGFHA GWRKSGIGGA DGKHGLHEYL QTQVVYLQS
Length	479
Mol. Wt	52.272 kDa
pI	4.9 (calculated)
Extinction coefficient	59,360 - 59,735 (calc based on 14 Y, 7 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P25553
Domain	15..475	PF00171 Aldehyde dehydrogenase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aldA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129376	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945672	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004725	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P25553	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10035	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10035	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945672	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000032	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0034	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	6.49E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	125.054+/-1.427	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.2523+/-0.05409	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.259207783	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 EMG2	280	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: Ml25	PMID: 9298646
Protein	E. coli K-12 MG1655	555	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1620	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	349	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aldA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1486236..1486276 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1415 (EcoliWiki Page)	NCBI GEO profiles for aldA
microarray	GenExpDB:b1415 (EcoliWiki Page)	Summary of data for aldA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1485974..1486312) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ03; Well:G6^[7]
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Notes

Accessions Related to aldA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10035	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0034	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1415
EcoGene	EcoGene:EG10035	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000032	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004725	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	ALDA	From SHIGELLACYC
E. coli O157	ALDA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00171 Aldehyde dehydrogenase family
Panther (EcoliWiki Page)	PTHR11699:SF37
Superfamily (EcoliWiki Page)	SUPERFAMILY:53720
EcoCyc	EcoCyc:EG10035	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10035	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000032	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0034	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004725	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

aldA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aldA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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