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R286W
R286W
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Growth Phenotype
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gain of function, can suppress cell division mutants, can compensate for a loss of ZipA
gain of function, can suppress cell division mutants, can compensate for a loss of ZipA
Below normal levels of FtsZ, normal cell division is not permitted
Cells are 27% shorter during exponential growth at 32C
Enhances Z-ring assembly kinetics without increasing total levels of FtsZ
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*PMID:12634424  
*PMID:12634424  
*PMID:16194242
*PMID:16194242
PMID:17542921
PMID:17542921
PMID:17322202
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Revision as of 18:40, 17 November 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ftsA

Mnemonic

Filamentation, temperature sensitive

Synonyms

ECK0095, b0094, JW0092, divA[1], divA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

2.24 minutes 

MG1655: 103982..105244
<gbrowseImage> name=NC_000913:103982..105244 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 106786..108048
<gbrowseImage> name=NC_012967:106786..108048 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 103981..105243
<gbrowseImage> name=NC_012759:103981..105243 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 103982..105244
<gbrowseImage> name=AC_000091:103982..105244 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 78086..79348
<gbrowseImage> name=NC_010473:78086..79348 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

FtsA*

R286W

Growth Phenotype

gain of function, can suppress cell division mutants, can compensate for a loss of ZipA

Below normal levels of FtsZ, normal cell division is not permitted

Cells are 27% shorter during exponential growth at 32C

Enhances Z-ring assembly kinetics without increasing total levels of FtsZ

PMID:17542921[4] PMID:17322202[5]

suppressor of loss of zipA
also suppresses

  • loss of ftsK
  • filamentation from MinC overproduction

ftsA1882(ts)

Growth Phenotype

forms small chains of swollen cells at 42 C; after a shift back to permissive temperature minicell-like cells are formed by the formation of aberrant constrictions

PMID:1097423[6] PMID:4591963[7] PMID:338600[8]

CGSC:10693

part of AX621 strain

ftsA16(Am)

Nonsense Mutation

Growth Phenotype

division stops after about 20 minutes at 42 C

PMID:374336[9]

CGSC:25115

amber (UAG) mutation

ftsA2(ts)

temperature sensitive

CGSC:25120

ftsA3(ts)

temperature sensitive

PMID:6365891[10]

CGSC:25123

forms abortive septa

ftsA27(ts)

temperature sensitive

CGSC:91085

ftsA6

PMID:3280547[11]

does not react to permissive temperature like other mutations

ftsA12(ts)

Growth Phenotype

stops dividing at 42 C


PMID:8509333[12]

PMID:7885232[13]

PMID:8509333[12]

CGSC:91083

ftsA12(ts)

Cell Shape

makes non-septated filaments at 42 C

PMID:8509333[12]

PMID:7885232[13]

CGSC:91083

ftsA10

Growth Phenotype

resistant to UV irradiation a temperature sensitive cell division mutant

PMID:14237063[14] PMID:3032902[15]

ftsA16(Am)

Nonsense Mutation

Cell Shape

at 42 C indentations where septation would occur if grown at permissive temperature are seen

PMID:374336[9]

CGSC:25115

amber (UAG) mutation

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0092

Plasmid clone

Shigen

PMID:16769691[16]

Status:Clone OK

Primer 1:GCCATCAAGGCGACGGACAGAAA

Primer 2:CCAAACTCTTTTCGCAGCCAACT

6F3

Kohara Phage

Genobase

PMID:3038334[17]

15B8

Kohara Phage

Genobase

PMID:3038334[17]

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[18]

est. P1 cotransduction: 47% [19]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[18]

est. P1 cotransduction: 14% [19]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:EG10339

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG10339

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944778

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Geissler, B et al. (2003) A gain-of-function mutation in ftsA bypasses the requirement for the essential cell division gene zipA in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 100 4197-202 PubMed
  3. ↑ Geissler, B & Margolin, W (2005) Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol. Microbiol. 58 596-612 PubMed
  4. ↑ Bernard, CS et al. (2007) An altered FtsA can compensate for the loss of essential cell division protein FtsN in Escherichia coli. Mol. Microbiol. 64 1289-305 PubMed
  5. ↑ Geissler, B et al. (2007) The ftsA* gain-of-function allele of Escherichia coli and its effects on the stability and dynamics of the Z ring. Microbiology (Reading, Engl.) 153 814-25 PubMed
  6. ↑ Walker, JR et al. (1975) Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation. J. Bacteriol. 123 693-703 PubMed
  7. ↑ Allen, JS et al. (1974) Regulation of bacterial cell division: genetic and phenotypic analysis of temperature-sensitive, multinucleate, filament-forming mutants of Escherichia. J. Bacteriol. 117 978-86 PubMed
  8. ↑ Fletcher, G et al. (1978) Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. J. Bacteriol. 133 91-100 PubMed
  9. ↑ 9.0 9.1 Lutkenhaus, JF & Donachie, WD (1979) Identification of the ftsA gene product. J. Bacteriol. 137 1088-94 PubMed
  10. ↑ Tormo, A & Vicente, M (1984) The ftsA gene product participates in formation of the Escherichia coli septum structure. J. Bacteriol. 157 779-84 PubMed
  11. ↑ Taschner, PE et al. (1988) Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments. J. Bacteriol. 170 1533-40 PubMed
  12. ↑ 12.0 12.1 12.2 Dai, K et al. (1993) Cloning and characterization of ftsN, an essential cell division gene in Escherichia coli isolated as a multicopy suppressor of ftsA12(Ts). J. Bacteriol. 175 3790-7 PubMed
  13. ↑ 13.0 13.1 Cook, WR & Rothfield, LI (1994) Development of the cell-division site in FtsA- filaments. Mol. Microbiol. 14 497-503 PubMed
  14. ↑ VAN DE PUTTE, P et al. (1964) THE SELECTION OF MUTANTS OF ESCHERICHIA COLI WITH IMPAIRED CELL DIVISION AT ELEVATED TEMPERATURE. Mutat. Res. 106 121-8 PubMed
  15. ↑ Descoteaux, A & Drapeau, GR (1987) Regulation of cell division in Escherichia coli K-12: probable interactions among proteins FtsQ, FtsA, and FtsZ. J. Bacteriol. 169 1938-42 PubMed
  16. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  17. ↑ 17.0 17.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  18. ↑ 18.0 18.1 CGSC: The Coli Genetics Stock Center
  19. ↑ 19.0 19.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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